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Lipidomics studies of recombinant Pichia pastoris for improved recombinant protein secretion through cell engineering
Metabolic engineering of glycosylation and secretion pathways in Pichia pastoris and its application in production of th
The Power of Pichia
Full-length versus truncated α-factor secretory signal sequences for expression of recombinant human insulin precursor i
[Transcriptome analysis of Pichia pastoris in response to ethanol stress].
Expression and characterization of a cutinase (AnCUT2) from Aspergillus niger
Biologically active recombinant carp LH as a spawning-inducing agent for carp
Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production
Truncated Prosequence of Rhizopus oryzae Lipase: Key Factor for Production Improvement and Biocatalyst Stability
Effect of Pyruvate Decarboxylase Knockout on Product Distribution Using Pichia pastoris (Komagataella phaffii) Engineere
Identification of a Novel Antibacterial Function of Mammalian Calreticulin.
Co-Expression of Pig IL-2 and Fusion Bovine Cathelicidin Gene by Recombinant Plasmids in Yeast and Their Promotion of Mo
Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii
Efficient Secretory Expression for Mammalian Hemoglobins in Pichia pastoris
A Universal Strategy for the Efficient Expression of Nanobodies in Pichia pastoris
High-Level Secretory Production of Recombinant E2-Spy Antigen Protein via Combined Strategy in Pichia pastoris
Discovery and Heterologous Expression of Unspecific Peroxygenases
Enhancing Antimicrobial Peptide Productivity in Pichia pastoris (Muts Strain) by Improving the Fermentation Process Base
Hybrid Deep Modeling of a GS115 (Mut+) Pichia pastoris Culture with State–Space Reduction
Process Simulation and Techno-Economic Analysis of Large-Scale Bioproduction of Sweet Protein Thaumatin II
Genetically Modified Micro-Organisms for Industrial Food Enzyme Production: An Overview
Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pasto
Production of a Recombinant Single-Domain Antibody for Gluten Detection in Foods Using the Pichia pastoris Expression Sy
Engineering and Expression Strategies for Optimization of L-Asparaginase Development and Production
Efficient Heterologous Production of Rhizopus oryzae Lipase via Optimization of Multiple Expression-Related Helper Prote
Production of a Granulysin-Based, Tn-Targeted Cytolytic Immunotoxin Using Pulsed Electric Field Technology
Optimized Expression and Characterization of a Novel Fully Human Bispecific Single-Chain Diabody Targeting Vascular Endo
Production of a Recombinant Non-Hydroxylated Gelatin Mimetic in Pichia pastoris for Biomedical Applications
High-Level Production of a Thermostable Mutant of Yarrowia lipolytica Lipase 2 in Pichia pastoris
Enhancing the Heterologous Expression of a Thermophilic Endoglucanase and Its Cost-Effective Production in Pichia pastor
Structural Homology Fails to Predict Secretion Efficiency in Pichia pastoris: Divergent Responses of Architecturally Sim
Yeast Genomics and Its Applications in Biotechnological Processes: What Is Our Present and Near Future?
Fundamental and Applicative Aspects of the Unfolded Protein Response in Yeasts
Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production
The Model System Saccharomyces cerevisiae Versus Emerging Non-Model Yeasts for the Production of Biofuels
Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories
A Highly Efficient Indirect P. pastoris Surface Display Method Based on the CL7/Im7 Ultra-High-Affinity System
Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
Application of Continuous Culture Methods to Recombinant Protein Production in Microorganisms
Production and Purification of Soy Leghemoglobin from Pichia pastoris Cultivated in Different Expression Media
Optimization of a Recombinant Lectin Production in Pichia pastoris Using Crude Glycerol in a Fed-Batch System
Comparison of Yeasts as Hosts for Recombinant Protein Production
Improved Production of Recombinant Carboxylesterase FumDM by Co-Expressing Molecular Chaperones in Pichia pastoris
Large-Scale Purification and Characterization of Recombinant Receptor-Binding Domain (RBD) of SARS-CoV-2 Spike Protein E
Comparison of Candida antarctica Lipase B Variants for Conversion of ε-Caprolactone in Aqueous Medium—Part 2
Non-conventional yeasts as superior production platforms for sustainable fermentation based bio-manufacturing processes
Engineering of a Microbial Cell Factory for the Extracellular Production of Catalytically Active Phospholipase A2 of Str
Enhanced Production of Soluble <i>Pyrococcus furiosus</i> α-Amylase in <i>Bacillus subtilis</i> through Chaperone Co-Exp
Overcoming Obstacles in Protein Expression in the Yeast Pichia pastoris: Interviews of Leaders in the Pichia Field
Prediction of strain engineerings that amplify recombinant protein secretion through the machine learning approach MaLPH
Engineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice
Comparative Proteome Analysis in Schizosaccharomyces pombe Identifies Metabolic Targets to Improve Protein Production an
Improved production of recombinant Rhizomucor miehei lipase by coexpressing protein folding chaperones in Pichia pastori
A novel phytase from Citrobacter gillenii : characterization and expression in Pichia pastoris ( Komagataella pastoris )
Recent advances in the production of recombinant subunit vaccines in Pichia pastoris.
A yeast for all seasons – Is Pichia pastoris a suitable chassis organism for future bioproduction?
The unfolded protein response in Pichia pastoris without external stressing stimuli.
What makes Komagataella phaffii non-conventional?
Systems biotechnology for protein production in Pichia pastoris.
Recombinant protein production in <i>Pichia pastoris</i>: from transcriptionally redesigned strains to bioprocess optimi
Advancing recombinant protein expression in Komagataella phaffii: opportunities and challenges.
CRISPR/Cas system for yeast genome engineering: advances and applications
Metabolic engineering strategies for improvement of ethanol production in cellulolytic Saccharomyces cerevisiae
Yeast-based vaccines: New perspective in vaccine development and application
Enhancing the production of a heterologous <i>Trametes</i> laccase (LacA) by replacement of the major cellulase CBH1 in
Yeast as a tool for membrane protein production and structure determination
Recombination machinery engineering facilitates metabolic engineering of the industrial yeast <i>Pichia pastoris</i>
Engineering microbial phenotypes through rewiring of genetic networks
Deglycosylated RBD produced in Pichia pastoris as a low-cost sera COVID-19 diagnosis tool and a vaccine candidate.
The impact of ERAD on recombinant protein secretion in Pichia pastoris (syn Komagataella spp.).
Structural insights into protein folding, stability and activity using in vivo perdeuteration of hen egg-white lysozyme.
Improvement of homologous GH10 xylanase production by deletion of genes with predicted function in the <i>Aspergillus ni
Biosynthesis of High‐Active Hemoproteins by the Efficient Heme‐Supply Pichia Pastoris Chassis
Camelid-derived heavy-chain nanobody against Clostridium botulinum neurotoxin E in Pichia pastoris.
Customizing amino acid metabolism of Pichia pastoris for recombinant protein production
Engineering a Highly Regioselective Fungal Peroxygenase for the Synthesis of Hydroxy Fatty Acids
Microscale Perfusion‐Based Cultivation for <i>Pichia pastoris</i> Clone Screening Enables Accelerated and Optimized Reco
High‐activity recombinant human carboxypeptidase B expression in Pichia pastoris through rational protein engineering an
Biotechnology and Bioengineering: Volume 116, Number 2, February 2019
RSM optimization of HSA/IL1Ra in Pichia pastoris overexpression strain and study of its in vivo activity in reducing hyp
Metabolic modeling to identify engineering targets for <i>Komagataella phaffii</i>: The effect of biomass composition on
Effect of gene copy number and chaperone coexpression on recombinant hydrophobin HFBI biosurfactant production in Pichia
Biosensor-assisted engineering of a high-yield Pichia pastoris cell-free protein synthesis platform.
Superior protein titers in half the fermentation time: Promoter and process engineering for the glucose‐regulated <i>GTH
Characterization of methanol utilization negative <i>Pichia pastoris</i> for secreted protein production: New cultivatio
Challenging the workhorse: Comparative analysis of eukaryotic micro‐organisms for expressing monoclonal antibodies
Modular development enables rapid design of media for alternative hosts
Genetic engineering approaches to improve posttranslational modification of biopharmaceuticals in different production p
Comparative genome‐scale analysis of <i>Pichia pastoris</i> variants informs selection of an optimal base strain
Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor-binding domain in engineered Komagataella phaffii.
Pichia pastoris: A highly successful expression system for optimal synthesis of heterologous proteins
Multiple Strategies for Recombinant Expression of Human Chymotrypsinogen C in
Heterologous Protein Expression in Pichia pastoris: Latest Research Progress and Applications.
Hypersecretory production of glucose oxidase in Pichia pastoris through combinatorial engineering of protein properties,
High-Throughput Optimization of Recombinant Protein Production in Microfluidic Gel Beads.
Investigating the effect of carbon source on rabies virus glycoprotein production in<i>Pichia pastoris</i>by a transcrip
<i>Pichia pastoris</i> protease‐deficient and auxotrophic strains generated by a novel, user‐friendly vector toolbox for
Effects of co-overexpression of secretion helper factors on the secretion of a HSA fusion protein (IL2-HSA) in pichia pa
Recombinant Allergens Production in Yeast.
Selection of the Optimal Yeast Host for the Synthesis of Recombinant Enzymes.
Direct Extraction and Purification of Recombinant Membrane Proteins from Pichia pastoris Protoplasts.
Protein Targeting Signals and Strategies for Pichia pastoris.
Effect of cooperation of chaperones and gene dosage on the expression of porcine PGLYRP-1 in Pichia pastoris.
Engineering of deglycosylated and plasmin resistant variants of recombinant streptokinase in Pichia pastoris
The future of recombinant host defense peptides
A Novel Application Potential of GH6 Cellobiohydrolase CtCel6 from Thermophilic Chaetomium thermophilum for Gene Cloning
Gene and process level modulation to overcome the bottlenecks of recombinant proteins expression in Pichia pastoris.
Secretory expression of human insulin precursor in Pichia pastoris employing truncated α-factor leader sequence and a sh
Pentapeptide Prosequence Enhances Expression and Structure Folding of Recombinant Thermomyces lanuginosus Lipase in Pich
Molecular Modification of Kex2 P1’ Site Enhances Expression and Druggability of Fungal Defensin
Green Production and Biotechnological Applications of Cell Wall Lytic Enzymes
Platforms for Production of Protein-Based Vaccines: From Classical to Next-Generation Strategies
Tuning Fatty Acid Profile and Yield in Pichia pastoris
Pichia pastoris (Komagataella phaffii) as a Cost-Effective Tool for Vaccine Production for Low- and Middle-Income Countr
Fusion Partner Facilitates Expression of Cell-Penetrating Peptide L2 in Pichia pastoris
Strategies for Optimizing the Production of Proteins and Peptides with Multiple Disulfide Bonds
Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Pro
Komagataella phaffii Erp41 is a protein disulfide isomerase with unprecedented disulfide bond catalyzing activity when c
A Novel Unspecific Peroxygenase from Galatian marginata for Biocatalytic Oxyfunctionalization Reactions
Refined Pichia pastoris reference genome sequence.
Synthetic activation of yeast stress response improves secretion of recombinant proteins.
Heterologous expression of the human polybromo-1 protein in the methylotrophic yeast Pichia pastoris.
Improved production and characterization of Volvariella volvacea Endoglucanase 1 expressed in Pichia pastoris.
Expression of recombinant tachyplesin I in Pichia pastoris
Molecular chaperones: A revolutionary approach for increased solubility of recombinant mAbs from bacterial and yeast sys
Recombinant expression of a laccase from Coriolopsis gallica in Pichia pastoris using a modified α-factor preproleader.
Optimized expression of the Starmerella bombicola lactone esterase in Pichia pastoris through temperature adaptation, co
Glycosylation is important for FcXTH1 activity as judged by its structural and biochemical characterization.
The combined effect of the gene copy number and chaperone overexpression on the recombinant bovine chymosin production i
Enhanced production of stem bromelain in Pichia pastoris by coexpression of unfolded protein response activator gene HAC
Genome and metabolic engineering in non-conventional yeasts: Current advances and applications.
Expression of soluble, glycosylated and correctly folded dengue virus NS1 protein in Pichia pastoris.
The effect of the bgs13 mutation on the structure of the reporter protein beta-lactoglobulin: Influence on folding and a
Ribo-seq guided design of enhanced protein secretion in Komagataellaphaffii.
Molecular engineering of insulin for recombinant expression in yeast
Pushing and pulling proteins into the yeast secretory pathway enhances recombinant protein secretion.
Heterologous Single-Strand DNA-Annealing and Binding Protein Enhance CRISPR-Based Genome Editing Efficiency in Komagatae
Structural and Biochemical Studies Enlighten the Unspecific Peroxygenase from <i>Hypoxylon</i> sp. EC38 as an Efficient
Impact of Oxygen Availability on the Organelle-Specific Redox Potentials and Stress in Recombinant Protein Producing Kom
The Impact of Methanol Concentration on Recombinant Protein Glycosylation in Pichia pastoris SuperMan5.
Fine‐tuning the <i>P. pastoris</i> iMT1026 genome‐scale metabolic model for improved prediction of growth on methanol or
Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia
Paving the way for the production of secretory proteins by yeast cell factories
Redox Engineering by Ectopic Overexpression of NADH Kinase in Recombinant Pichia pastoris (Komagataella phaffii): Impact
Molecular optimization of rabies virus glycoprotein expression in Pichia pastoris.
Isolation of a laccase-coding gene from the lignin-degrading fungus<i>Phlebia brevispora</i>BAFC 633 and heterologous ex
Characterization of recombinant human lysosomal beta-hexosaminidases produced in the methylotrophic yeast Pichia pastori
Knockout of RSN1, TVP18 or CSC1-2 causes perturbation of Golgi cisternae in Pichia pastoris.
Functional Expression of Two Unusual Acidic Peroxygenases from Candolleomyces aberdarensis in Yeasts by Adopting Evolved
A Novel Colletotrichum graminicola Raffinose Oxidase in the AA5 Family
Plackett-Burman Design for rGILCC1 Laccase Activity Enhancement in<i> Pichia pastoris</i>: Concentrated Enzyme Kinetic C
Functional Characterization of the N-Terminal C2 Domain from<i>Arabidopsis thaliana</i>Phospholipase D<i>α</i>and D<i>β<
Insights on Heterologous Expression of Fungal Cellulases in &lt;i&gt;Pichia pastoris&lt;/i&gt;
A Novel Thermostable GH3<i>β</i>-Glucosidase from<i>Talaromyce leycettanus</i>with Broad Substrate Specificity and Signi
Improved Production of Aspergillus usamii endo- β -1,4-Xylanase in Pichia pastoris via Combined Strategies
Production Optimization of an Active β -Galactosidase of Bifidobacterium animalis in Heterologous Expression Systems
Impact of disulfide bonds on the folding and refolding capability of a novel thermostable GH45 cellulase.
Pathway engineering facilitates efficient protein expression in Pichia pastoris.
Overexpressing target helper genes enhances secretion and glycosylation of recombinant proteins in Pichia pastoris under
Heterologous expression and characterization of the aspartic endoprotease Pep4um from Ustilago maydis, a homolog of the
Rapid screening of cellular stress responses in recombinant <i>Pichia pastoris</i> strains using metabolite profiling
Molecular assembly of recombinant chicken type II collagen in the yeast Pichia pastoris.
Effect of Fold-Promoting Mutation and Signal Peptide Screening on Recombinant Glucan 1,4-Alpha-maltohydrolase Secretion
High-level expression of Thermomyces dupontii thermo-alkaline lipase in Pichia pastoris under the control of different p
Optimization of the secretory expression of recombinant human C-reactive protein in Pichia pastoris
Microbial proteases: ubiquitous enzymes with innumerable uses
N-glycosylation and homodimeric folding significantly enhance the immunoreactivity of Mycobacterium tuberculosis virulen
Free fatty acids reduce metabolic stress and favor a stable production of heterologous proteins in Pichia pastoris.
Directed evolution of the aryl-alcohol oxidase: Beyond the lab bench
Metabolomics-based development of bioproduction processes toward industrial-scale production
Preparation of gentiooligosaccharides using Trichoderma viride β-glucosidase.
Deglycosylation significantly affects the activity, stability and appropriate folding of recombinant Aspergillus niger α
Efficient heterologous expression of nicotinate dehydrogenase in Comamonas testosteroni CNB-2 with transcriptional, fold
Improvement of extracellular bacterial protein production in Pichia pastoris by co-expression of endoplasmic reticulum r
An engineered Pichia pastoris platform for the biosynthesis of silk-based nanomaterials with therapeutic potential.
Defining the mechanism of PDI interaction with disulfide-free amyloidogenic proteins: Implications for exogenous protein
A multistrategy approach for improving the expression of E. coli phytase in Pichia pastoris
Screening endogenous signal peptides and protein folding factors to promote the secretory expression of heterologous pro
Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris
Second generation Pichia pastoris strain and bioprocess designs
A novel recombinant chimeric bio-adhesive protein consisting of mussel foot protein 3, 5, gas vesicle protein A, and Csg
Expressing anti-HIV VRC01 antibody using the murine IgG1 secretion signal in Pichia pastoris.
Identification and characterization of N-glycosylation site on a Mucor circinelloides aspartic protease expressed in Pic
Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolis
Expression, characterization, and application of human-like recombinant gelatin
Comprehensive reconstruction and evaluation of Pichia pastoris genome-scale metabolic model that accounts for 1243 ORFs
Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris
Expression, characterization, and application potentiality evaluation of recombinant human-like collagen in Pichia pasto
Role of N-linked glycosylation in the enzymatic properties of a thermophilic GH 10 xylanase from Aspergillus fumigatus e
Xenobiotic Compounds Degradation by Heterologous Expression of a Trametes sanguineus Laccase in Trichoderma atroviride
A Recombinant Human Anti-Platelet scFv Antibody Produced in Pichia pastoris for Atheroma Targeting
Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with R
Cofactor engineering improved CALB production in Pichia pastoris through heterologous expression of NADH oxidase and ade
Production of an anti-Aβ antibody fragment in Pichia pastoris and in vitro and in vivo validation of its therapeutic eff
Cloning, expression, and characterization of a recombinant xylanase from Bacillus sonorensis T6
Recombinant expression, purification and biochemical characterization of kievitone hydratase from Nectria haematococca
Expression and purification of FGF21 in Pichia pastoris and its effect on fibroblast-cell migration
Recombinant human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in the methylotrophic yeast Pichia pastoris
High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization,
Increased dosage of AOX1 promoter-regulated expression cassettes leads to transcription attenuation of the methanol meta
Large scale microfluidic CRISPR screening for increased amylase secretion in yeast
Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor-binding domain in engineered Komagataella phaffii.
Structural and Functional Comparison of SARS-CoV-2-Spike Receptor Binding Domain Produced in <i>Pichia pastoris</i> and
The SARS-CoV-2 receptor-binding domain expressed in Pichia pastoris as a candidate vaccine antigen
A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases
Effects of glycerol supply and specific growth rate on methanol-free production of CALB by P. pastoris: functional chara
A novel strategy to improve protein secretion via overexpression of the SppA signal peptide peptidase in Bacillus lichen
Establishment of Neurospora crassa as a host for heterologous protein production using a human antibody fragment as a mo
The effect of hypoxia on the lipidome of recombinant Pichia pastoris
A novel methanol-free Pichia pastoris system for recombinant protein expression
Directed evolution and secretory expression of a pyrethroid-hydrolyzing esterase with enhanced catalytic activity and th
Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica
A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris
Production of fluorescent and cytotoxic K28 killer toxin variants through high cell density fermentation of recombinant
Enhanced extracellular expression of Bacillus stearothermophilus α-amylase in Bacillus subtilis through signal peptide o
Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker
High cell density fed-batch production of insecticidal recombinant ribotoxin hirsutellin A from Pichia pastoris
CRISPR–Cas9-mediated genomic multiloci integration in Pichia pastoris
Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins
Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella ph
Differential role of segments of α-mating factor secretion signal in Pichia pastoris towards granulocyte colony-stimulat
Genome-based engineering of ligninolytic enzymes in fungi
Establishment of a functional system for recombinant production of secreted proteins at 50 °C in the thermophilic Bacill
A glucotolerant β-glucosidase from the fungus Talaromyces amestolkiae and its conversion into a glycosynthase for glycos
Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up
Expression of antibody fragments in Saccharomyces cerevisiae strains evolved for enhanced protein secretion
Engineering Saccharomyces cerevisiae for the production and secretion of Affibody molecules
Simultaneous enzyme production, Levan-type FOS synthesis and sugar by-products elimination using a recombinant Pichia pa
Transcriptomic profiling of the yeast Komagataella phaffii in response to environmental alkalinization
Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection
Impact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitn
‘Mother(Nature) knows best’ – hijacking nature-designed transcriptional programs for enhancing stress resistance and pro
Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activi
Expression and characterization of cold-adapted xylanase Xyl-L in Pichia pastoris for xylooligosaccharide (XOS) preparat
Engineering a highly active thermophilic β-glucosidase to enhance its pH stability and saccharification performance
Redesigning N-glycosylation sites in a GH3 β-xylosidase improves the enzymatic efficiency
Characterization and modulation of endoplasmic reticulum stress response target genes in Kluyveromyces marxianus to impr
Novel molecular biological tools for the efficient expression of fungal lytic polysaccharide monooxygenases in Pichia pa
Functional and biological insights of rCollinein-1, a recombinant serine protease from Crotalus durissus collilineatus
Improving production of Streptomyces griseus trypsin for its application in processing insulin precursor
Translational Modification and pH Optimization of Expression Media for High-Yield Recombinant Human-like Collagen (RHC)
Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Re
Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free PDH promoter
Establishment and application of an RNAi system in Pichia pastoris
Systematic Optimization of Protein Secretory Pathways in Saccharomyces cerevisiae to Increase Expression of Hepatitis B
Exploring the Synergy between Cellobiose Dehydrogenase from Phanerochaete chrysosporium and Cellulase from Trichoderma r
Pichia pastoris-Expressed Bivalent Virus-Like Particulate Vaccine Induces Domain III-Focused Bivalent Neutralizing Antib
A Novel Vector for Construction of Markerless Multicopy Overexpression Transformants in Pichia pastoris
Production of Chimeric Acidic α-Amylase by the Recombinant Pichia pastoris and Its Applications
Biochemical analyses of a novel acidophilic GH5 β-mannanase from Trichoderma asperellum ND-1 and its application in mann
Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus β-Glucosidase in Pichia
Plectasin: from evolution to truncation, expression, and better druggability
Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care
RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes
Improving recombinant protein production by yeast through genome-scale modeling using proteome constraints
Engineered bidirectional promoters enable rapid multi-gene co-expression optimization
A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris
Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris
High-level heterologous production and Functional Secretion by recombinant Pichia pastoris of the shortest proline-rich
Designing strong inducible synthetic promoters in yeasts
The effect of non-thermal atmospheric plasma on the production and activity of recombinant phytase enzyme
Enhanced cell density cultivation and rapid expression-screening of recombinant Pichia pastoris clones in microscale
Cloning, characterization, and heterologous expression of a candidate Hirudin gene from the salivary gland transcriptome
FACS-Based Functional Protein Screening via Microfluidic Co-encapsulation of Yeast Secretor and Mammalian Reporter Cells
Pichia pastoris growth—coupled heme biosynthesis analysis using metabolic modelling
Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammali
A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella
Predicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indica
Cold atmospheric plasma treatment enhances recombinant model protein production in yeast Pichia pastoris
Expression and function of an Hac1-regulated multi-copy xylanase gene in Saccharomyces cerevisiae
Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermenta
Enhancement of thermoalkaliphilic xylanase production by Pichia pastoris through novel fed-batch strategy in high cell-d
An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibri
Dynamic genome-scale metabolic modeling of the yeast Pichia pastoris
Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris
Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcripti
Integration event induced changes in recombinant protein productivity in Pichia pastoris discovered by whole genome sequ
Expressing Cloned Genes for Protein Production, Purification, and Analysis
Production of protein‐based polymers in Pichia pastoris
Microbial Cyclodextrin Glycosyltransferases: Sources, Production, and Application in Cyclodextrin Synthesis
Production and secretion dynamics of prokaryotic Penicillin G acylase in Pichia pastoris
Characterization of a thermotolerant aryl-alcohol oxidase from Moesziomyces antarcticus oxidizing 5-hydroxymethyl-2-fura
FeedER: a feedback-regulated enzyme-based slow-release system for fed-batch cultivation in microtiter plates
Molecular characterization, purification, and antioxidant activity of recombinant superoxide dismutase from the Pacific
The recombinant pea defensin Drr230a is active against impacting soybean and cotton pathogenic fungi from the genera Fus
Cloning and expression of Saccharomyces cerevisiae SUC2 gene in yeast platform and characterization of recombinant enzym
Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in ye
Efficient production and characterization of the novel and highly active antifungal protein AfpB from Penicillium digita
A novel, highly efficient β-glucosidase with a cellulose-binding domain: characterization and properties of native and r
Huoqing; Su, Xiaoyun
suxiaoyun@caas.cn
Simultaneous manipulation of multiple genes within a same regulatory stage for iterative evolution of Trichoderma reesei
ViewYan; Yu, Xiao‐Wei
yuxw@jiangnan.edu.cn
From induction to secretion: a complicated route for cellulase production in Trichoderma reesei
ViewJian; Kang, Zhen
zkang@jiangnan.edu.cn
Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy
ViewNavone, Laura; Moffitt
laura.navone@qut.edu.au
Bioengineered textiles with peptide binders that capture SARS-CoV-2 viral particles
ViewSynergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastor
ViewBiosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris
ViewBrigitte; Mattanovich, Diethard
diethard.mattanovich@boku.ac.at
The bud tip is the cellular hot spot of protein secretion in yeasts.
ViewStephan; Gasser, Brigitte; Mattanovich
brigitte.gasser@boku.ac.at
Increasing pentose phosphate pathway flux enhances recombinant protein production in Pichia pastoris
ViewDisruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant protein
ViewGoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris
ViewClaudia; Glieder, Anton; Garcia‐Ortega
a.glieder@tugraz.at
Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9
ViewKerry R.; Love, J. Christopher
clove@mit.edu
Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candi
ViewSteric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella
ViewRuiming; Li, Lu; Guo
feylin@scut.edu.cn
Enhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins in Pichia pastor
ViewHigh-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia past
Viewydhuang@fjnu.edu.cn
The α-mating factor secretion signals and endogenous signal peptides for recombinant protein secretion in Komagataella p
natalia.kwiatos@p.lodz.pl
Improvement of efficiency of brown coal biosolubilization by novel recombinant Fusarium oxysporum laccase
syhan@scut.edu.cn
Efficient expression of an alkaline pectin lyase from Bacillus licheniformis in Pichia pastoris
zhaozb@dicp.ac.cn
Secretory expression of β-1,3-glucomannanase in the oleaginous yeast Rhodosporidium toruloides for improved lipid extrac
yli@im.ac.cn
Enhancing the secretion pathway maximizes the effects of mixed feeding strategy for glucose oxidase production in the me
guoreyting@hubu.edu.cn
Functional tailoring of a PET hydrolytic enzyme expressed in Pichia pastoris
ktakeda@ongene.med.osaka-u.ac.jp
Human LYPD8 protein inhibits motility of flagellated bacteria
liang@jlu.edu.cn
Transcriptional Regulation of Aerobic Metabolism in Pichia pastoris Fermentation
dominique.michaud@fsaa.ulaval.ca
An Accessory Protease Inhibitor to Increase the Yield and Quality of a Tumour-Targeting mAb in Nicotiana benthamiana Lea
zpshi@jiangnan.edu.cn
Enhancing monellin production by Pichia pastoris at low cell induction concentration via effectively regulating methanol
lutz.schmitt@hhu.de
Cloning and expression of selected ABC transporters from the Arabidopsis thaliana ABCG family in Pichia pastoris
b-shokri@sbu.ac.ir
Simulation and experimental study of a cold atmospheric pressure plasma and comparison of efficiency in boosting recombi
fengxingjun2008@163.com
Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin-2 in Pichia pastoris
dnurdiani@gmail.com
Enhancement in Human Insulin Precursor Secretion by Pichia pastoris through Modification of Expression Conditions
xszhou@ecust.edu.cn
Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shi
ming.li@mq.edu.au
Droplet flow cytometry for single-cell analysis
r.aw08@imperial.ac.uk
Liquid PTVA: a faster and cheaper alternative for generating multi-copy clones in Pichia pastoris
alexander.frey@aalto.fi
Enhancing antibody folding and secretion by tailoring the Saccharomyces cerevisiae endoplasmic reticulum
denise.salzig@lse.thm.de
A high-throughput expression screening platform to optimize the production of antimicrobial peptides
martin.dragosits@boku.ac.at
Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in
Hui.Wei@nrel.gov
Expression and secretion of fungal endoglucanase II and chimeric cellobiohydrolase I in the oleaginous yeast Lipomyces s
marc.nicaud@inra.fr
New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica
verhaert@proteonic.nl
Presence of protein production enhancers results in significantly higher methanol-induced protein production in Pichia p
bsglick@uchicago.edu
An improved secretion signal enhances the secretion of model proteins from Pichia pastoris
oliver.spadiut@tuwien.ac.at
Production of a recombinant peroxidase in different glyco-engineered Pichia pastoris strains: a morphological and physio
kristel.bernaerts@kuleuven.be
Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein
lhzhang@scau.edu.cn
Co-expressing GroEL–GroES, Ssa1–Sis1 and Bip–PDI chaperones for enhanced intracellular production and partial-wall break
henisha@hubu.edu.cn
Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant g
mianzhou@ecust.edu.cn
Codon usage bias regulates gene expression and protein conformation in yeast expression system P. pastoris.
xiaow@ustc.edu.cn
Development of IL-15/IL-15Rα sushi domain-IgG4 Fc complexes in Pichia pastoris with potent activities and prolonged half
zhangdongcumt@163.com
Isolation, heterologous expression, and purification of a novel antifungal protein from Bacillus subtilis strain Z-14
kongyun@sdu.edu.cn
Homogeneous production and characterization of recombinant N-GlcNAc-protein in Pichia pastoris
talva001@ucr.edu
Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii.
liudehu2006@126.com
The introduction of an N-glycosylation site into prochymosin greatly enhances its production and secretion by Pichia pas
luohuiying@caas.cn
Engineering a carbohydrate-binding module to increase the expression level of glucoamylase in Pichia pastoris
nccrook@ncsu.edu
Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineerin
Peter.schotte@sanofi.com
Optimizing expression of Nanobody® molecules in Pichia pastoris through co-expression of auxiliary proteins under methan
julian.kopp@tuwien.ac.at
Bi-directionalized promoter systems allow methanol-free production of hard-to-express peroxygenases with Komagataella Ph
sandra.castillo@vtt.fi
Whole-genome metabolic model of Trichoderma reesei built by comparative reconstruction
vincent.martin@concordia.ca
Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae
chenxiuzhen@im.ac.cn
Identification of two integration sites in favor of transgene expression in Trichoderma reesei
min.zhang@nrel.gov
Expression of an endoglucanase–cellobiohydrolase fusion protein in Saccharomyces cerevisiae, Yarrowia lipolytica, and Li
tho@gate.sinica.edu.tw
Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia past
wang_qh@tib.cas.cn
Microfluidic screening and genomic mutation identification for enhancing cellulase production in Pichia pastoris
snurbayaoslan@upm.edu.my
Molecular expression of a recombinant thermostable bacterial amylase from Geobacillus stearothermophilus SR74 using meth
manpalsridhar@yahoo.co.uk
Enhanced delignification of lignocellulosic substrates by <i>Pichia</i> GS115 expressed recombinant laccase
luowen@ms.giec.ac.cn
Expression in Pichia pastoris and characterization of Rhizomucor miehei lipases containing a new propeptide region.
liyi@hubu.edu.cn
A Combinational Strategy for Effective Heterologous Production of Functional Human Lysozyme in Pichia pastoris
mkwon@gnu.ac.kr
Developing methylotrophic microbial platforms for a methanol-based bioindustry
gaohui@szbl.ac.cn
In situ intein-mediated multiprotein assembly via engineered cross-species consortia
yaoyu@fudan.edu.cn
High-level expression of leghemoglobin in Kluyveromyces marxianus by remodeling the heme metabolism pathway
nicolas.papon@univ-angers.fr
Yeasts as Biopharmaceutical Production Platforms
paul.barlow@ed.ac.uk
Murine Factor H Co-Produced in Yeast With Protein Disulfide Isomerase Ameliorated C3 Dysregulation in Factor H-Deficient
alessandro.pellis@boku.ac.at
Enzymatic Degradation of Aromatic and Aliphatic Polyesters by P. pastoris Expressed Cutinase 1 from Thermobifida cellulo
zhangwei02@caas.cn
Decreased Rhamnose Metabolic Flux Improved Production of Target Proteins and Cell Flocculation in Pichia pastoris
jiangwei01@cau.edu.cn
Insight to Improve α-L-Arabinofuranosidase Productivity in Pichia pastoris and Its Application on Corn Stover Degradatio
maria.coca@cragenomica.es
Biotechnological Production of the Cell Penetrating Antifungal PAF102 Peptide in Pichia pastoris
biwangjianrong@mail.scut.edu.cn
Overexpression and Biochemical Properties of a GH46 Chitosanase From Marine Streptomyces hygroscopicus R1 Suitable for C
fangh@zju.edu.cn
Current advances of Pichia pastoris as cell factories for production of recombinant proteins
jwhe@lnu.edu.cn
Comparative transcriptomic analysis-based identification of the regulation of foreign proteins with different stabilitie
wangyongtao102@cau.edu.cn
Production of a Novel Superoxide Dismutase by Escherichia coli and Pichia pastoris and Analysis of the Thermal Stability
nielsenj@chalmers.se
Efficient protein production by yeast requires global tuning of metabolism
gregory.batt@inria.fr
Maximizing protein production by keeping cells at optimal secretory stress levels using real-time control approaches
Katrien.Claes@vib-ugent.be
OPENPichia: licence-free Komagataella phaffii chassis strains and toolkit for protein expression
juchu@ecust.edu.cn
Multi-omics integrative analysis with genome-scale metabolic model simulation reveals global cellular adaptation of Aspe
pfickers@ULiege.be
Comprehensive comparison of Yarrowia lipolytica and Pichia pastoris for production of Candida antarctica lipase B
malixing@hubu.edu.cn
Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational
lloyd.ruddock@oulu.fi
Biochemical analysis of Komagataella phaffii oxidative folding proposes novel regulatory mechanisms of disulfide bond fo
xuanweizhou@sjtu.edu.cn
Expression and characteristics of manganese peroxidase from Ganoderma lucidum in Pichia pastoris and its application in
gaurav.batra@thsti.res.in
Casamino acids facilitate the secretion of recombinant dengue virus serotype-3 envelope domain III in Pichia pastoris
sunjianan@ouc.edu.cn
A novel autolysis system for extracellular production and direct immobilization of a phospholipase D fused with cellulos
linjingwei1980@163.com
Recombinant Expression and Bioactivity Comparison of Four Typical Fungal Immunomodulatory Proteins from Three Main Ganod
andrea_camattari@bti.a
Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein p
nico.callewaert@vib-ugent.be
Massively parallel interrogation of protein fragment secretability using SECRiFY reveals features influencing secretory
martin.weissenborn@ipb-halle.de
A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases
gerald.lackner@uni-bayreuth.de
Engineering Saccharomyces cerevisiae for medical applications
fengwei01@lcu.edu.cn
Microbial proteases and their applications
d.j.leak@bath.ac.uk
Insights into the prevalence and underlying causes of clonal variation through transcriptomic analysis in Pichia pastori
vlada.urlacher@uni-duesseldorf.de
High-level expression of aryl-alcohol oxidase 2 from Pleurotus eryngii in Pichia pastoris for production of fragrances a
Engineering protein translocation and unfolded protein response enhanced human PH-20 secretion in Pichia pastoris
Katja.Koschorreck@hhu.de
Two adjacent C-terminal mutations enable expression of aryl-alcohol oxidase from Pleurotus eryngii in Pichia pastoris.
zhiweizhang2012@163.com
Site-directed mutation of β-galactosidase from Aspergillus candidus to reduce galactose inhibition in lactose hydrolysis
asshan@neau.edu.cn
Design and heterologous expression of a novel dimeric LL37 variant in Pichia pastoris