allg · 515 papers with claims · 352 for outreach · 142 old · 21 reviews
<2016
Filtered out
Preparative‐Scale Production of Testosterone Metabolites by Human Liver Cytochrome P450 Enzyme 3A4
Biosynthesis of High‐Active Hemoproteins by the Efficient Heme‐Supply <i>Pichia Pastoris</i> Chassis
Accurate and cost-effective prediction of HBsAg titer in industrial scale fermentation process of recombinant Pichia pas
A fluorescent screening method for optimization of conotoxin expression in Pichia pastoris.
Co‐Expression of Chaperones for Improvement of Recombinant <i>Geobacillus stearothermophilus</i> Lipase Production in <i
A New Strategy of Alternating Current Magnetic Field-Assisted Three-Phase Partitioning (ACMF-TPP) for the Concentration
Microscale Perfusion‐Based Cultivation for <i>Pichia pastoris</i> Clone Screening Enables Accelerated and Optimized Reco
Customizing amino acid metabolism of <i>Pichia pastoris</i> for recombinant protein production
Facilitating secretory expression of apple seed β-glucosidase in Komagataella phaffii for the efficient preparation of s
Metabolic modeling to identify engineering targets for <i>Komagataella phaffii</i>: The effect of biomass composition on
Development of a general defined medium for Pichia pastoris.
Methanol independent induction in Pichia pastoris by simple derepressed overexpression of single transcription factors.
Process development for the continuous production of heterologous proteins by the industrial yeast, <i>Komagataella phaf
Challenging the workhorse: Comparative analysis of eukaryotic micro‐organisms for expressing monoclonal antibodies
Engineering of alcohol dehydrogenase 2 hybrid-promoter architectures in Pichia pastoris to enhance recombinant protein e
Comparative genome‐scale analysis of <i>Pichia pastoris</i> variants informs selection of an optimal base strain
Characterization of methanol utilization negative <i>Pichia pastoris</i> for secreted protein production: New cultivatio
Modeling of copy number variability in Pichia pastoris
Modular development enables rapid design of media for alternative hosts
Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor-binding domain in engineered Komagataella phaffii.
Minimal purification method enables developability assessment of recombinant proteins
Development of a high-throughput microscale cell disruption platform for Pichia pastoris in rapid bioprocess design.
Biotransformation of β‐hydroxypyruvate and glycolaldehyde to l ‐erythrulose by <i>Pichia pastoris</i> strain GS115 overe
Holistic process development to mitigate proteolysis of a subunit rotavirus vaccine candidate produced in Pichia pastori
Expression and Purification of Recombinant Human Type III Collagen using the N-terminal Transdermal Peptide in Pichia pa
Cloning and Overexpressing Membrane Proteins Using <i>Pichia pastoris (Komagataella phaffii)</i>
<i>Pichia pastoris</i>: A highly successful expression system for optimal synthesis of heterologous proteins
Recombinant expression of
Transfer of disulfide bond formation modules via yeast artificial chromosomes promotes the expression of heterologous pr
Scorpion insect neurotoxin LqhIT2 is a promising oral biopesticide: high-level preparation in Pichia pastoris and bioact
<i>Pichia pastoris</i> protease‐deficient and auxotrophic strains generated by a novel, user‐friendly vector toolbox for
Pichia pastoris Continuous Cultivations for Strain Characterization and Continuous Biomanufacturing.
Off-Gas Balancing for Bioprocess Monitoring Pichia pastoris Bioprocesses.
Demonstration-Scale High-Cell-Density Fermentation of Pichia pastoris.
High-Throughput Screening and Selection of Pichia pastoris Strains.
Engineering and fermenter production of fungi GLA in Pichia pastoris GS115 using oil waste.
Improving heterologous expression of porcine follicle-stimulating hormone in Pichia pastoris by integrating molecular st
High-level constitutive expression of leech hyaluronidase with combined strategies in recombinant Pichia pastoris.
Transcriptional control of gene expression in Pichia pastoris by manipulation of terminators.
Is heterogeneity in large-scale bioreactors a real problem in recombinant protein synthesis by Pichia pastoris?
A thermostable and inhibitor resistant β-glucosidase from Rasamsonia emersonii for efficient hydrolysis of lignocellulos
Influence of methanol/sorbitol co-feeding rate on pAOX1 induction in a Pichia pastoris Mut+ strain in bioreactor with li
A comparative study of glycerol and sorbitol as co-substrates in methanol-induced cultures of Pichia pastoris: temperatu
Rapid screening of cellular stress responses in recombinant <i>Pichia pastoris</i> strains using metabolite profiling
Reduced methanol input induces increased protein output by AOX1 promoter in a trans-acting elements engineered Pichia pa
Production of Industrial Enzymes via Pichia pastoris as a Cell Factory in Bioreactor: Current Status and Future Aspects.
Engineering Pichia pastoris for Efficient Production of a Novel Bifunctional Strongylocentrotus purpuratus Invertebrate-
Production of a Recombinant α-L-Rhamnosidase from Aspergillus niger CCTCC M 2018240 in Pichia pastoris.
High-Level Expression and Biochemical Characterization of a Maltotetraose Amylase in Pichia pastoris X-33 for Maltotetra
Increase of Candida antarctica lipase B production under PGK promoter in Pichia pastoris: effect of multicopies
Simultaneously promoting gellan lyase expression and gellan oligosaccharide production by gellan gum co-feeding during P
Bioproduced Proteins On Demand (Bio-POD) in hydrogels using Pichia pastoris.
High-yield secretory production of stable, active trypsin through engineering of the N-terminal peptide and self-degrada
Recent progress in microbial biosurfactants production strategies: Applications, technological bottlenecks, and future o
Construction of an efficient enzyme-cell@material biocatalyst through the biofilm immobilization of Komagataella phaffii
Carbon metabolism influenced for promoters and temperature used in the heterologous protein production using Pichia past
Production, purification, and characterization of recombinant rabies virus glycoprotein expressed in PichiaPink™ yeast.
Designing and developing a sensitive and specific SARS-CoV-2 RBD IgG detection kit for identifying positive human sample
Overexpression of Candida rugosa lipase Lip1 via combined strategies in Pichia pastoris.
Sorbitol/methanol mixed induction reduces process impurities and improves centrifugal dewatering in Pichia pastoris cult
A metabolic-engineering framework approach via fed-batch fermentation for enhancing glucaric acid production in Komagata
Heterologous expression and biochemical characterization of a novel thermostable Sclerotinia sclerotiorum GH45 endogluca
Combined effect of gene dosage and process optimization strategies on high-level production of recombinant human interle
Assessing virus like particles formation and r-HBsAg aggregation during large scale production of recombinant hepatitis
An engineered Pichia pastoris platform for the biosynthesis of silk-based nanomaterials with therapeutic potential.
Integrated strategies for efficient production of Streptomyces mobaraensis transglutaminase in Komagataella phaffii.
Expression, optimization and biological activity analysis of recombinant type III collagen in Komagataella phaffii.
Codon-optimized production of Cu/Zn superoxide dismutase (SOD1) in Komagataella phaffii: Functional characterization and
Gel microdroplet-based high-throughput screening for directed evolution of xylanase-producing Pichia pastoris.
Efficient production of human goose-type lysozyme 2 in the methylotrophic yeast Pichia pastoris.
Removal of bacterial dextran in sugarcane juice by Talaromyces minioluteus dextranase expressed constitutively in Pichia
Engineering of the hypoxia-induced Pichia stipitis ADH2 promoter to construct a promoter library for Pichia pastoris.
Rational development of bioprocess engineering strategies for recombinant protein production in Pichia pastoris (Komagat
Cloning, expression and functional characterization of two sesquiterpene synthase genes from moso bamboo (Phyllostachys
Comparison of ADH3 promoter with commonly used promoters for recombinant protein production in Pichia pastoris.
High-yield fermentation and a novel heat-precipitation purification method for hydrophobin HGFI from Grifola frondosa in
Efficient expression and isolation of recombinant human interleukin-11 (rhIL-11) in Pichia pastoris.
High-level extracellular production of Rhizopus oryzae lipase in Pichia pastoris via a strategy combining optimization o
Efficient production of a recombinant Venerupis philippinarum defensin (VpDef) in Pichia pastoris and characterization o
Expression of recombinant tachyplesin I in Pichia pastoris.
Extracellular production of the recombinant bacterial transglutaminase in Pichia pastoris.
Highly secretory expression of recombinant cowpea chlorotic mottle virus capsid proteins in Pichia pastoris and in-vitro
Cloning and characterization of a novel DNase gene from Trichogramma pretiosum.
Expression and biochemical characterization of a Bacillus subtilis catalase in Pichia pastoris X-33.
High performance production process development and scale-up of an anti-TSLP nanobody.
Innovative Tools and Strategies for Optimizing Yeast Cell Factories.
Versatile xylose and arabinose genetic switches development for yeasts.
Numerical Simulation of Biomass Growth in OKTOP®9000 Reactor at Industrial Scale.
Efficient Conversion of Inulin to Inulooligosaccharides through Endoinulinase from Aspergillus niger.
Ethanol-Inducible Bioproduction of Human α-Lactalbumin in Komagataella phaffii.
Synthesis of Isomalto-Oligosaccharides by Pichia pastoris Displaying the Aspergillus niger α-Glucosidase.
Construction of an l-Tyrosine Chassis in Pichia pastoris Enhances Aromatic Secondary Metabolite Production from Glycerol
Microbial engineering for the production of C2-C6 organic acids.
Engineered SARS-CoV-2 receptor binding domain improves manufacturability in yeast and immunogenicity in mice
Enhancement of recombinant BmK AngM1 production in Pichia pastoris by regulating gene dosage, co-expressing with chapero
Improving thermal hysteresis activity of antifreeze protein from recombinant Pichia pastoris by removal of N-glycosylati
Enhancing granulocyte colony-stimulating factor expression in Pichia pastoris through fusion with human serum albumin.
Large-scale purification of recombinant hepatitis B surface antigen from Pichia pastoris with non-affinity chromatograph
Application of recurrent neural network for online prediction of cell density of recombinant Pichia pastoris producing H
Dextranase production by recombinant Pichia pastoris under operational volumetric mass transfer coefficient (kLa) and vo
High purity of human secreted phospholipase A2 group IIE in Pichia pastoris using basal salts medium comparison with YPD
Expression and purification of an engineered, yeast-expressed Leishmania donovani nucleoside hydrolase with immunogenic
Recombinant expression of hen egg white lysozyme with the assistance of xylanase fusion partner in Pichia pastoris.
A yeast for all seasons – Is Pichia pastoris a suitable chassis organism for future bioproduction?
Recombinant protein production in <i>Pichia pastoris</i>: from transcriptionally redesigned strains to bioprocess optimi
Unraveling the potential of non-conventional yeasts in biotechnology
Engineering the expression system for <i>Komagataella phaffii (Pichia pastoris)</i>: an attempt to develop a methanol-fr
Free fatty acids promote transformation efficiency of yeast
Impact of overexpressing NADH kinase on glucoamylase production in <i>Aspergillus niger</i>
Characterization of the off-flavor from <i>Pichia pastoris</i> GS115 during the overexpression of an α- l -rhamnosidase
Characterization of the exopolysaccharides produced by the industrial yeast Komagataella phaffii.
Benchmarking recombinant <i>Pichia</i> <i>pastoris</i> for 3‐hydroxypropionic acid production from glycerol
Synergic kinetic and physiological control to improve the efficiency of Komagataella phaffii recombinant protein product
Development of a Recombinant Omicron BA.1 Subunit Vaccine Candidate in Pichia pastoris.
Exploring Yeast's Energy Dynamics: The General Stress Response Lowers Maintenance Energy Requirement
Combinatorial Alkali‐Responsive Hybrid Promoters as Tools for Heterologous Protein Expression in <i>Saccharomyces cerevi
The Impact of Methanol Concentration on Recombinant Protein Glycosylation in Pichia pastoris SuperMan5
Evaluación de la enzima alfa-N-acetilglucosaminidasa humana recombinante obtenida en Komagataella phaffii GS115 en un mo
Functional Expression of Two Unusual Acidic Peroxygenases from Candolleomyces aberdarensis in Yeasts by Adopting Evolved
Insights on Heterologous Expression of Fungal Cellulases in &lt;i&gt;Pichia pastoris&lt;/i&gt;
[Synthesis of vitamin K2 by isopentenyl transferase NovA in Pichia pastoris Gpn12].
[One-pot synthesis of chondroitin sulfate A by engineered Pichia pastoris].
[Expression, purification and bioactivity analysis of a recombinant fusion protein rHSA-hFGF21 in Pichia pastoris].
[Expression and biological characterization of anti-CD63 single-chain variable fragment antibody in Pichia pastoris].
Pichia pastoris: SEL RAGI UNTUK PRODUKSI PROTEIN REKOMBINAN
Lipid Composition of <i>Sheffersomyces stipitis</i> M12 Strain Grown on Glycerol as a Carbon Source
Evaluating Pulses and Modified Fed-batch Feeding of Methanol to Increase Expression Level of Human Insulin Precursor in
Technical transfer of a rapid microbial platform for vaccine production
Development of a strategy induction AOX1 promoter methylotrophic yeast Komagataella phaffii
A seven-transmembrane methyltransferase catalysing N-terminal histidine methylation of lytic polysaccharide monooxygenas
Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii
Gene and process level modulation to overcome the bottlenecks of recombinant proteins expression in Pichia pastoris.
Cofactor engineering for efficient production of α-farnesene by rational modification of NADPH and ATP regeneration path
Microfluidic Microbioreactor to Reduce the Cost and Speed Up Optimisation of Protein Production
Farklı pH ve sıcaklık değerlerinin Pichia pastoris'de rekombinant ekspanzin üretimi üzerine etkisi
A comparison of different constitutive expression systems for the production of bovine chymosin in Komagataella phaffii
Implementing Chemostat Fermentation of Pichia Pastoris Producing Recombinant HBsAg to Optimize Cell Density Affected by
Methanol Independent Expression by <em>Pichia Pastoris</em> Employing De-repression Technologies
Heterologous Expression and Characterization of a Thermostable Exo-β-D-Glucosaminidase from Aspergillus oryzae.
Improving 3'-Hydroxygenistein Production in Recombinant Pichia pastoris Using Periodic Hydrogen Peroxide-Shocking S
Developing strategies for systems metabolic engineering of Pichia pastoris
[Engineering of a System for the Production of Mutant Human Alpha-Fetoprotein in the Methylotrophic Yeast Pichia pastori
THE DEVELOPMENT OF HUMAN INSULIN PRECURSOR EXPRESSED FROM P.PASTORIS -X33 IN A 10L-FERMENTER
Production and secretion dynamics of prokaryotic Penicillin G acylase in Pichia pastoris
Impact of overproduced heterologous protein characteristics on physiological response in Yarrowia lipolytica steady-stat
Process development and scale-up optimization of the SARS-CoV-2 receptor binding domain-based vaccine candidate, RBD219-
Characterization of a thermotolerant aryl-alcohol oxidase from Moesziomyces antarcticus oxidizing 5-hydroxymethyl-2-fura
Two adjacent C-terminal mutations enable expression of aryl-alcohol oxidase from Pleurotus eryngii in Pichia pastoris
Aromatic secondary metabolite production from glycerol was enhanced by amino acid addition in Pichia pastoris
Efficient secretory expression of type III recombinant human collagen with triple-helical structure in Komagataella phaf
Heterologous expression and optimization of the antimicrobial peptide acidocin 4356 in Komagataella phaffii to target Ps
Immunisation with Neospora caninum subunits rsNcSAG4 and rsNcGRA1 (NcSAG4 and NcGRA1 epitopes construct) in BALB/c mice:
A simple and effective method to remove pigments from heterologous secretory proteins expressed in Pichia pastoris.
Synthetic biology and microbioreactor platforms for programmable production of biologics at the point-of-care
Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae
Applications, challenges, and needs for employing synthetic biology beyond the lab
A roadmap to establish a comprehensive platform for sustainable manufacturing of natural products in yeast
Designing strong inducible synthetic promoters in yeasts
OPENPichia: licence-free Komagataella phaffii chassis strains and toolkit for protein expression
A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris
Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on tra
Structural and functional comparison of SARS-CoV-2-spike receptor binding domain produced in Pichia pastoris and mammali
Predicting high recombinant protein producer strains of Pichia pastoris MutS using the oxygen transfer rate as an indica
Unravelling the hidden power of esterases for biomanufacturing of short-chain esters
Biochemical analysis of Komagataella phaffii oxidative folding proposes novel regulatory mechanisms of disulfide bond fo
A streamlined strain engineering workflow with genome-wide screening detects enhanced protein secretion in Komagataella
A DNA assembly toolkit to unlock the CRISPR/Cas9 potential for metabolic engineering
Scaling-up Fermentation of Pichia pastoris to demonstration-scale using new methanol-feeding strategy and increased air
Improved Production of Active Streptomyces griseus Trypsin with a Novel Auto-Catalyzed Strategy
Non-canonical integration events in Pichia pastoris encountered during standard transformation analysed with genome sequ
Methanol-Independent Protein Expression by AOX1 Promoter with trans-Acting Elements Engineering and Glucose-Glycerol-Shi
Development of a Pichia pastoris whole-cell biocatalyst with overexpression of mutant lipase I PCLG47I from Penicillium
Expression of <sup>2</sup>H, <sup>13</sup>C, <sup>15</sup>N-labeled NIST-Fab fragment in the methylotrophic yeast <i>Kom
Benchmarking non-targeted metabolomics using yeast derived libraries
A combined flow injection/reversed phase chromatography – high resolution mass spectrometry workflow for accurate absolu
LyGo: A platform for rapid screening of lytic polysaccharide monooxygenase production
Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candi
Engineered SARS-CoV-2 receptor binding domain improves immunogenicity in mice and elicits protective immunity in hamster
Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii.
Modular development enables rapid design of media for alternative hosts
P <sub> <i>AOX1</i> </sub> expression in mixed-substrate continuous cultures of <i>Komagataella phaffii</i> ( <i>Pichia
Steric accessibility of the <i>N</i> -terminus improves the titer and quality of recombinant proteins secreted from <i>K
A membrane integral methyltransferase catalysing N-terminal histidine methylation of lytic polysaccharide monooxygenases
Characterization of Recombinant Human Lactoferrin Expressed in <i>Komagataella Phaffii</i>
Yeast-based production platform for potent and stable heavy chain-only antibodies
Efficient production of itaconic acid from the single carbon substrate methanol with engineered <i>Komagataella phaffii<
Flux balance analysis predicts NADP phosphatase and NADH kinase are critical to balancing redox during xylose fermentati
The Mut <sup>+</sup> strain of <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) expresses P <i> <sub>AOX1</sub> </
Mutational Analysis of Ocriplasmin to Reduce Proteolytic and Autolytic Activity in Pichia pastoris
Comparative genomics and transcriptomics of Pichia pastoris
Comparative transcriptome and metabolome analyses reveal the methanol dissimilation pathway of Pichia pastoris
Production and purification of chimeric HBc virus-like particles carrying influenza virus LAH domain as vaccine candidat
Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcripti
An effective combination of codon optimization, gene dosage, and process optimization for high-level production of fibri
Genome-scale model reconstruction of the methylotrophic yeast Ogataea polymorpha
Heterologous expression of nattokinase from B. subtilis natto using Pichia pastoris GS115 and assessment of its thrombol
Functional inclusion bodies produced in the yeast Pichia pastoris
Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in
Biomarkers allow detection of nutrient limitations and respective supplementation for elimination in Pichia pastoris fed
A novel bi-directional promoter system allows tunable recombinant protein production in Pichia pastoris
Presence of protein production enhancers results in significantly higher methanol-induced protein production in Pichia p
Disruption of vacuolar protein sorting components of the HOPS complex leads to enhanced secretion of recombinant protein
Efficient expression vectors and host strain for the production of recombinant proteins by Yarrowia lipolytica in proces
Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella ph
Secretion of a low and high molecular weight β-glycosidase by Yarrowia lipolytica
Multiple strategies to improve the yield of chitinase a from Bacillus licheniformis in Pichia pastoris to obtain plant g
Translational landscape and protein biogenesis demands of the early secretory pathway in Komagataella phaffii
Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastor
Bioprocess performance analysis of novel methanol-independent promoters for recombinant protein production with Pichia p
Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9
Molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine candi
Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up.
Metabolic engineering of Pichia pastoris for myo-inositol production by dynamic regulation of central metabolism
From strain engineering to process development: monoclonal antibody production with an unnatural amino acid in Pichia pa
Steric accessibility of the N-terminus improves the titer and quality of recombinant proteins secreted from Komagataella
Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris
Engineering Escherichia coli for efficient assembly of heme proteins
Transcriptomic profiling of the yeast Komagataella phaffii in response to environmental alkalinization
Biosensor-guided rapid screening for improved recombinant protein secretion in Pichia pastoris
High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availab
Economical production of Pichia pastoris single cell protein from methanol at industrial pilot scale
Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic a
Protein production dynamics and physiological adaptation of recombinant Komagataella phaffii at near-zero growth rates
Impact of cell wall polysaccharide modifications on the performance of Pichia pastoris: novel mutants with enhanced fitn
Unlocking Nature’s Toolbox: glutamate-inducible recombinant protein production from the Komagatella phaffii PEPCK promot
Harnessing alkaline-pH regulatable promoters for efficient methanol-free expression of enzymes of industrial interest in
Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection
Evaluation of heterologous expression in Pichia pastoris of Pine Weevil TRPA1 by GFP and flow cytometry
Engineering Pichia pastoris for the efficient production of the high-value steroid intermediate 15α-OH-D-ethylgonendione
Rewiring central metabolism in Komagataella phaffii for efficient mannose synthesis.
Metabolic engineering of Komagataella phaffii for enhanced 3-hydroxypropionic acid (3-HP) production from methanol.
Genome wide analysis of protein production load in Trichoderma reesei
Proximity ligation scaffolding and comparison of two Trichoderma reesei strains genomes
Enhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins in Pichia pastor
Modulation of acetate utilization in Komagataella phaffii by metabolic engineering of tolerance and metabolism
Chaetomella raphigera β-glucosidase D2-BGL has intriguing structural features and a high substrate affinity that renders
Modular biosynthesis of plant hemicellulose and its impact on yeast cells
Oxidative stress tolerance contributes to heterologous protein production in Pichia pastoris
Second generation Pichia pastoris strain and bioprocess designs
Enhancing D-lactic acid production by optimizing the expression of D-LDH gene in methylotrophic yeast Komagataella phaff
Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermoph
Physiological characterization and transcriptome analysis of Pichia pastoris reveals its response to lignocellulose-deri
Scalable recombinant production of bioactive human neutrophil peptide-1 in Komagataella phaffii.
Fungal statin pump protein improves monacolin J efflux and regulates its production in Komagataella phaffii
Secretory expression of cyclohexanone monooxygenase by methylotrophic yeast for efficient omeprazole sulfide bio-oxidati
Metabolic engineering of methylotrophic Pichia pastoris for the production of β-alanine
Transcription regulation strategies in methylotrophs: progress and challenges
Development of a Pichia pastoris cell factory for efficient production of germacrene A: a precursor of β-elemene
Efficient expression of an alkaline pectin lyase from Bacillus licheniformis in Pichia pastoris
Improved microscale cultivation of Pichia pastoris for clonal screening.
Enhanced Co-culture System Using <i>Escherichia coli</i> and <i>Pichia pastoris</i> (<i>Komagataella phaffii</i>) for Im
In silico and in vitro analysis of THCA synthase gene in Moroccan Cannabis sativa, L
Exploring the genetic architecture of protein secretion in Komagataella phaffii (Pichia pastoris) for biotechnology appl
Structure of SALO, a leishmaniasis vaccine candidate from the sand fly Lutzomyia longipalpis.
Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without
Population genomics shows no distinction between pathogenic Candida krusei and environmental Pichia kudriavzevii: One sp
Real-time monitoring of recombinant GFP expression in single-cell <i>Komagataella phaffii</i> through filamentous pellet
Rekombinante Produktion eines Kollagen-mimetischen Proteins in Komagataella phaffii (syn. Pichia pastoris)
The Smell of Synthetic Biology: Engineering Strategies for Aroma Compound Production in Yeast
Constitutive High Expression Level of A Synthetic Deleted Encoding Gene of <i>Talaromyces minioluteus</i> Endodextranase
Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain (RBD
Production of Plant-Based Meat Flavor Compound – Leghemo-Globin (LegH) in Recombinant <em>Komagataella phaffii</em> Fed-
Analysis of a thermostable lipase from CTG-clade yeast: Molecular expression, characterization and structure prediction
- **Authors:** Silva, Paulo César, Johansson, B. - **Year:** 2024 - **Journal:** Journal UMinho Science - **Source:** op
Establishing site-specific recombination mediated cassette exchange in Pichia pastoris and development of an optimized p
Development of human insulin precursor expressed from P.pastoris-X33 in a 10L-fermenter
Komagataella phaffii (Pichia pastoris) as a Powerful Yeast Expression System for Biologics Production
A Combinational Strategy for Effective Heterologous Production of Functional Human Lysozyme in Pichia pastoris.
Automation of Dead End Filtration: An Enabler for Continuous Processing of Biotherapeutics.
Editorial: Recent Advances in Continuous Cultivation
Improved Production of Xylanase in Pichia pastoris and Its Application in Xylose Production From Xylan.
Advances and Opportunities of CRISPR/Cas Technology in Bioengineering Non-conventional Yeasts
Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separati
1Progress, applications, challenges and prospects of protein purification technology
Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Re
Enantioselective Biosynthesis of L-Phenyllactic Acid From Phenylpyruvic Acid In Vitro by L-Lactate Dehydrogenase Couplin
Combining Metabolic Engineering and Multiplexed Screening Methods for 3-Hydroxypropionic Acid Production in Pichia pasto
Fluorescence lifetime imaging of NAD(P)H upon oxidative stress in Kluyveromyces marxianus
PAOX1 expression in mixed-substrate continuous cultures of Komagataella phaffii (Pichia pastoris) is completely determin
Enabling growth-decoupled Komagataella phaffii recombinant protein production based on the methanol-free PDH promoter
Nitrogen recovery from low-value biogenic feedstocks via steam gasification to methylotrophic yeast biomass
Editorial: “The value of microbial bioreactors to meet challenges in the circular bioeconomy”
Engineering the methylotrophic yeast Ogataea polymorpha for lactate production from methanol
Plant Flavonoid Production in Bacteria and Yeasts
Insight to Improve α-L-Arabinofuranosidase Productivity in Pichia pastoris and Its Application on Corn Stover Degradatio
Single-Cell Approach to Monitor the Unfolded Protein Response During Biotechnological Processes With Pichia pastoris
Development of a New High-Cell Density Fermentation Strategy for Enhanced Production of a Fungus β-Glucosidase in Pichia
Diversifying Isoprenoid Platforms via Atypical Carbon Substrates and Non-model Microorganisms
Current advances of Pichia pastoris as cell factories for production of recombinant proteins
Comparative transcriptomic analysis-based identification of the regulation of foreign proteins with different stabilitie
Metabolic Fingerprint Analysis of Cytochrome b5-producing E. coli N4830-1 Using FT-IR Spectroscopy
The cell wall and the response and tolerance to stresses of biotechnological relevance in yeasts
Multiple strategies were adopted to optimize the enzymatic characteristics and improve the expression of bovine chymosin
From linoleic acid to hexanal and hexanol by whole cell catalysis with a lipoxygenase, hydroperoxide lyase and reductase
The advent of plant cells in bioreactors
Boosting Expression of a Specifically Targeted Antimicrobial Peptide K in Pichia pastoris by Employing a 2A Self-Cleavin
Strategies for Optimizing the Production of Proteins and Peptides with Multiple Disulfide Bonds
Broadening the Biocatalytic Toolbox—Screening and Expression of New Unspecific Peroxygenases
Yeast and Virus-like Particles: A Perfect or Imperfect Couple?
Tuning Fatty Acid Profile and Yield in Pichia pastoris
Current Understanding on the Heterogenous Expression of Plastic Depolymerising Enzymes in Pichia pastoris
Effect of Pyruvate Decarboxylase Knockout on Product Distribution Using Pichia pastoris (Komagataella phaffii) Engineere
Depletion of Boric Acid and Cobalt from Cultivation Media: Impact on Recombinant Protein Production with Komagataella ph
The Pentose Phosphate Pathway in Yeasts–More Than a Poor Cousin of Glycolysis
Preparative Production of Functionalized (N- and O-Heterocyclic) Polycyclic Aromatic Hydrocarbons by Human Cytochrome P4
Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii
Statistically Designed Medium Reveals Interactions between Metabolism and Genetic Information Processing for Production
Expression and Purification of Cp3GT: Structural Analysis and Modeling of a Key Plant Flavonol-3-O Glucosyltransferase f
Advances in Recombinant Lipases: Production, Engineering, Immobilization and Application in the Pharmaceutical Industry
Truncated Prosequence of Rhizopus oryzae Lipase: Key Factor for Production Improvement and Biocatalyst Stability
Performance of Recombinant Komagataella phaffii in Plant-Based Meat Flavor Compound-Leghemoglobin (LegH) Production thro
Efficient Secretory Expression for Mammalian Hemoglobins in Pichia pastoris
Modifications of Constitutive Promoter to Large-Scale Synthesize Porcine Myoglobin in Komagataella phaffii
Cutinase Production in Komagataella phaffii (Pichia pastoris): Performance Differences Between Host Strains
Nonconventional Yeasts Engineered Using the CRISPR-Cas System as Emerging Microbial Cell Factories
Enhancing Antimicrobial Peptide Productivity in Pichia pastoris (Muts Strain) by Improving the Fermentation Process Base
Development of a Cost-Effective Process for the Heterologous Production of SARS-CoV-2 Spike Receptor Binding Domain Usin
Extracellular Production of the Taiwan-Native Norovirus P Domain Overexpressed in Pichia pastoris
Effects of Temperature and pH on Recombinant Thaumatin II Production by Pichia pastoris.
Secretory Production of Plant Heme-Containing Globins by Recombinant Yeast via Precision Fermentation.
Transcriptomic Analysis of the Influence of Methanol Assimilation on the Gene Expression in the Recombinant Pichia pasto
High-Level Production of a Thermostable Mutant of Yarrowia lipolytica Lipase 2 in Pichia pastoris.
Heterologous Expression of Xylanase xAor from Aspergillus oryzae in Komagataella phaffii T07
Production of Bioactive Porcine Lactoferrin through a Novel Glucose-Inducible Expression System in Pichia pastoris: Unve
Just around the Corner: Advances in the Optimization of Yeasts and Filamentous Fungi for Lactic Acid Production
Production of Vespa tropica Hyaluronidase by Pichia pastoris
Metabolic Engineering of Pichia pastoris for the Production of Triacetic Acid Lactone
Fundamental and Applicative Aspects of the Unfolded Protein Response in Yeasts
Influence of Dissolved Oxygen Level on Chitin-Glucan Complex and Mannans Production by the Yeast Pichia pastoris.
Engineering of Synthetic Transcriptional Switches in Yeast
Optimization Workflow of Fumonisin Esterase Production for Biocatalytic Degradation of Fumonisin B1.
Advances in Cell Engineering of the Komagataella phaffii Platform for Recombinant Protein Production
Role of Dissimilative Pathway of Komagataella phaffii (Pichia pastoris): Formaldehyde Toxicity and Energy Metabolism
Enhanced Expression of Alcohol Dehydrogenase I in Pichia pastoris Reduces the Content of Acetaldehyde in Wines
Komagataella phaffii as a Platform for Heterologous Expression of Enzymes Used for Industry
Recombinant Inga Laurina Trypsin Inhibitor (ILTI) Production in Komagataella Phaffii Confirms Its Potential Anti-Biofilm
Comparison of Yeasts as Hosts for Recombinant Protein Production
Heterologous Hyaluronic Acid Production in Kluyveromyces lactis
Evaluation of Product Distribution in Chemostat and Batch Fermentation in Lactic Acid-Producing Komagataella phaffii Str
A Host-Vector System for the Expression of a Thermostable Bacterial Lipase in a Locally Isolated Meyerozyma guilliermond
Heterologous Expression of Ketoreductase ChKRED20 Mutant in Pichia pastoris and Bioreductive Production of (R)-1, 3-Buta
Standardization and Key Aspects of the Development of Whole Yeast Cell Vaccines
l-Asparaginase Type II from Fusarium proliferatum: Heterologous Expression and In Silico Analysis
Enhanced Stability of Lactobacillus paracasei Aspartate Ammonia-Lyase via Electrospinning for Enzyme Immobilization
Production and Purification of Soy Leghemoglobin from Pichia pastoris Cultivated in Different Expression Media
Improved Production of Recombinant Carboxylesterase FumDM by Co-Expressing Molecular Chaperones in Pichia pastoris
Large-Scale Purification and Characterization of Recombinant Receptor-Binding Domain (RBD) of SARS-CoV-2 Spike Protein E
Upgrading Non-Conventional Yeasts into Valuable Biofactories
High cell density fermentation strategies for the production of bovine lactoferrin in Pichia pastoris