Strain Engineering For Pichia Pastoris Protein Sec

Single‐chain antibody fragment production in Pichia pastoris: Benefits of prolonged pre‐induction glycerol feeding

N. K. Khatri; D. Gocke; Oliver Trentmann et al.

<i>Pichia pastoris</i> secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher

Maccani, Andreas; Landes, Nils; Stadlmayr, Gerhard et al.

Genome‐scale metabolic model of methylotrophic yeast <i>Pichia pastoris</i> and its use for <i>in silico</i> analysis of

Sohn, Seung Bum; Graf, Alexandra B.; Kim, Tae Yong et al.

Industrial biotechnology: Tools and applications

Tang, Weng Lin; Zhao, Huimin

Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris

Hubertus Hohenblum; Brigitte Gasser; Michael Maurer et al.

Genome‐scale metabolic model of <i>Pichia pastoris</i> with native and humanized glycosylation of recombinant proteins

Irani, Zahra Azimzadeh; Kerkhoven, Eduard J.; Shojaosadati, Seyed Abbas et al.

In Pichia pastoris, growth rate regulates protein synthesis and secretion, mating and stress response

C. Rebnegger; A. Graf; Minoska Valli et al.

Expression of recombinant vitellogenin in the yeast Pichia pastoris

J. Ding; E. H. Lim; H.F. Li et al.

Enhancement of protein secretion in Pichia pastoris by overexpression of protein disulfide isomerase.

Inan, Mehmet; Aryasomayajula, Dinesh; Sinha, Jayanta et al.

Economic analysis of royalactin production under uncertainty: Evaluating the effect of parameter optimization

Torres‐Acosta, Mario A.; Aguilar‐Yáñez, José Manuel; Rito‐Palomares, Marco et al

Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production

Rachel Daly; M. Hearn

Heterologous protein production using the Pichia pastoris expression system

Sue Macauley-Patrick; Mariana L. Fazenda; B. McNeil et al.

Crystal structure of a triacylglycerol lipase from <i>Penicillium expansum</i> at 1.3 Å determined by sulfur SAD

Bian, Chuanbing; Yuan, Cai; Chen, Liqing et al.

¹³C-based metabolic flux analysis of recombinant Pichia pastoris.

Ferrer, Pau; Albiol, Joan

Hygromycin-resistance vectors for gene expression in Pichia pastoris

Yang, Junjie; Nie, Lei; Chen, Biao et al.

Decrease of hirudin degradation by deleting the KEX1 gene in recombinant Pichia pastoris

Z. Ni; Xiangshan Zhou; Xueqian Sun et al.

Secreted production of an elastin-like polypeptide by Pichia pastoris

Schipperus, Roelof; Teeuwen, Rosalie L. M.; Werten, Marc W. T. et al.

Biosynthetic production of type II fish antifreeze protein: fermentation by Pichia pastoris

M. Loewen; X. Liu; P. Davies et al.

Production of Functionalized Single-Chain Fv Antibody Fragments Binding to the ED-B Domain of the B-isoform of Fibronect

Marty, Cornelia; Scheidegger, Patrick; Ballmer‐Hofer, Kurt et al.

Simple and efficient expression of Agaricus meleagris pyranose dehydrogenase in Pichia pastoris

Sygmund, Christoph; Gutmann, Alexander; Krondorfer, Iris et al.

Cooverexpression of chaperones for enhanced secretion of a single-chain antibody fragment in Pichia pastoris

Leonardo M. Damasceno; K. Anderson; G. Ritter et al.

Recombinant Aspergillus β-galactosidases as a robust glycomic and biotechnological tool

Dragosits, Martin; Pflügl, Stefan; Kurz, Simone et al.

A homologous production system for Trichoderma reesei secreted proteins in a cellulase-free background

Uzbaş, Fatma; Sezerman, Osman Uğur; Hartl, Lukas et al.

Investigating the physiological response of Pichia (Komagataella) pastoris GS115 to the heterologous expression of misfo

Hesketh, Andrew; Castrillo, Juan I.; Sawyer, Trevor et al.

Novel in vitro inhibitory functions of potato tuber proteinaceous inhibitors

Fischer, Matthias; Kuckenberg, Markus; Kastilan, Robin et al.

Real-time estimation of biomass and specific growth rate in physiologically variable recombinant fed-batch processes

Wechselberger, Patrick; Sagmeister, Patrick; Herwig, Christoph

Lignocellulose degrading extremozymes produced by Pichia pastoris: current status and future prospects

Burcu Gündüz Ergün; P. Çalık

A visual method for direct selection of high-producing Pichia pastorisclones

Hu, Fan; Li, Xin; Lü, Jie et al.

Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris

Nordén, Kristina; Agemark, Maria; Danielson, Jonas ÅH et al.

A new cold-adapted β-D-galactosidase from the Antarctic Arthrobacter sp. 32c – gene cloning, overexpression, purificatio

Hildebrandt, Piotr; Wanarska, Marta; Kur, Józef

Single chain Fab (scFab) fragment

Hust, Michael; Jostock, Thomas; Menzel, Christian et al.

The impact of oxygen on the transcriptome of recombinant S. cerevisiae and P. pastoris - a comparative analysis

Baumann, Kristin; Dato, Laura; Graf, Alexandra B. et al.

Functional expression of a blood tolerant laccase in Pichia pastoris

D. Maté; David Gonzalez-Perez; R. Kittl et al.

Cloning, sequence analysis, expression of Cyathus bulleri laccase in Pichia pastoris and characterization of recombinant

Garg, Neha; Bieler, Nora; Kenzom, T. et al.

The alkaline pectate lyase PEL168 of Bacillus subtilis heterologously expressed in Pichia pastorisis more stable and eff

Zhang, Chengjie; Yao, Jia; Zhou, Cheng et al.

Improved production of recombinant human Fas ligand extracellular domain in Pichia pastoris: yield enhancement using dis

M. Muraki

Systematic screening of glycosylation- and trafficking-associated gene knockouts in Saccharomyces cerevisiaeidentifies m

Wang, Tzi‐Yuan; Huang, Chih-Jen; Chen, Hsin‐Liang et al.

Efficient production of human bivalent and trivalent anti-MUC1 Fab-scFv antibodies in Pichia pastoris

Schoonooghe, Steve; Kaigorodov, V. A.; Zawisza, Monika et al.

A dynamic method based on the specific substrate uptake rate to set up a feeding strategy for Pichia pastoris

Dietzsch, Christian; Spadiut, Oliver; Herwig, Christoph

A thermostable GH45 endoglucanase from yeast: impact of its atypical multimodularity on activity

Couturier, Marie; Féliu, Julia; Haon, Mireille et al.

Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccha

Bey, Mathieu; Berrin, Jean‐Guy; Poidevin, Laetitia et al.

Assaying proline hydroxylation in recombinant collagen variants by liquid chromatography-mass spectrometry

Chan, Sam Wei Polly; Greaves, John; Silva, Nancy A. Da et al.

Heterologous overexpression of Glomerella cingulata FAD-dependent glucose dehydrogenase in Escherichia coli and Pichia p

Sygmund, Christoph; Staudigl, Petra; Klausberger, Miriam et al.

The response to unfolded protein is involved in osmotolerance of Pichia pastoris

Dragosits, Martin; Stadlmann, Johannes; Graf, Alexandra B. et al.

Enhanced thermostability of a Rhizopus chinensis lipase by in vivo recombination in Pichia pastoris

Yu, Xiao‐Wei; Wang, Rui; Zhang, Meng et al.

A fast approach to determine a fed batch feeding profile for recombinant Pichia pastoris strains

Dietzsch, Christian; Spadiut, Oliver; Herwig, Christoph

Optimization of production of the anti-keratin 8 single-chain Fv TS1-218 in Pichia pastoris using design of experiments

Jafari, Rozbeh; Sundström, Birgitta; Holm, Patrik

Virus-like particle production with yeast: ultrastructural and immunocytochemical insights into Pichia pastoris producin

Lünsdorf, Heinrich; Gurramkonda, Chandrasekhar; Adnan, Ahmad et al.

Modeling and measuring intracellular fluxes of secreted recombinant protein in Pichia pastoris with a novel 34S labeling

Pfeffer, Martin; Maurer, Michael; Köllensperger, Gunda et al.

Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastori

Kristin Baumann; Núria Adelantado; C. Lang et al.

Antifoam addition to shake flask cultures of recombinant Pichia pastoris increases yield

Routledge, Sarah J; Hewitt, Christopher J.; Bora, Nagamani et al.

Physiological response of Pichia pastoris GS115 to methanol-induced high level production of the Hepatitis B surface ant

Vanz, AnaLeticia; Lünsdorf, Heinrich; Adnan, Ahmad et al.

Metabolic flux profiling of recombinant protein secreting Pichia pastoris growing on glucose:methanol mixtures

Joel Jordà; P. Jouhten; Elena Cámara et al.

Expression of lignocellulolytic enzymes in Pichia pastoris

A. Mellitzer; R. Weis; A. Glieder et al.

Characterization of a thermostable β-glucosidase from Aspergillus fumigatus Z5, and its functional expression in Pichia

Liu, Dongyang; Zhang, Ruifu; Yang, Xingming et al.

Systems metabolic engineering, industrial biotechnology and microbial cell factories

Lee, Sang Yup; Mattanovich, Diethard; Villaverde, Antonio

Quantitative metabolomics analysis of amino acid metabolism in recombinant Pichia pastoris under different oxygen availa

Marc Carnicer; A. ten Pierick; J. V. van Dam et al.

Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae

Shiroishi, Mitsunori; Tsujimoto, Hirokazu; Makyio, Hisayoshi et al.

Enhanced membrane protein expression by engineering increased intracellular membrane production

Guerfal, Mouna; Claes, Katrien; Knittelfelder, Oskar et al.

Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

Chen, Ming‐Tang; Lin, Song; Shandil, Ishaan et al.

Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris

Jariyachawalid, Kanidtha; Laowanapiban, Poramaet; Meevootisom, Vithaya et al.

Overexpression of an exotic thermotolerant β-glucosidase in trichoderma reesei and its significant increase in celluloly

Dashtban, Mehdi; Qin, Wensheng

Recombinant sterol esterase from Ophiostoma piceae: an improved biocatalyst expressed in Pichia pastoris

Cedillo, Víctor Barba; Plou, Francisco J.; Martı́nez, Marı́a Jesús

Induction without methanol: novel regulated promoters enable high-level expression in Pichia pastoris

Prielhofer, Roland; Maurer, Michael; Klein, Joachim et al.

Decrease of UPR- and ERAD-related proteins in Pichia pastoris during methanol-induced secretory insulin precursor produc

Ana Letícia Vanz; M. Nimtz; U. Rinas

Towards better understanding of an industrial cell factory: investigating the feasibility of real-time metabolic flux an

Fazenda, Mariana L.; Dias, João M.L.; Harvey, Linda M. et al.

Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production

Gao, Limei; Cai, Menghao; Shen, Wei et al.

Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bio

J. Hemmerich; Núria Adelantado; J. M. Barrigón et al.

A quantitative study of methanol/sorbitol co-feeding process of a Pichia pastoris Mut+/pAOX1-lacZ strain

Niu, Hongxing; Jost, Laurent; Pirlot, Nathalie et al.

Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production

Sygmund, Christoph; Santner, Paul; Krondorfer, Iris et al.

Yeast biotechnology: teaching the old dog new tricks

Mattanovich, Diethard; Sauer, Michael; Gasser, Brigitte

Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragment from Corynebacteri

Matsuda, Yoshihiko; Itaya, Hiroshi; Kitahara, Yuki et al.

Expression of endoglucanases in Pichia pastoris under control of the GAP promoter

Várnai, Anikó; Tang, Campbell; Bengtsson, Oskar et al.

Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

Sauer, Michael; Branduardi, Paola; Gasser, Brigitte et al.

Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris

Gürkan, Cemal; Ellar, David J.

The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi

Joosten, Vivi; Lokman, Christien; Hondel, Cees A. M. J. J. van den et al.

Regulation of methanol utilisation pathway genes in yeasts.

Hartner, Franz Stefan; Glieder, Anton

Application of a wide-range yeast vector (CoMed™) system to recombinant protein production in dimorphic Arxula adeninivo

Steinborn, Gerhard; Böer, Erik; Scholz, Anja et al.

Heterologous expression of lcc1 gene from Trametes trogii in Pichia pastoris and characterization of the recombinant enz

Colao, M. Ch.; Lupino, Stefania; Garzillo, Anna Maria Vittoria et al.

Versatile modeling and optimization of fed batch processes for the production of secreted heterologous proteins with Pic

Maurer, Michael; Kühleitner, Manfred; Gasser, Brigitte et al.

Role of cultivation media in the development of yeast strains for large scale industrial use

Hahn‐Hägerdal, Bärbel; Karhumaa, Kaisa; Larsson, Christer et al.

Rivoflavin may interfere with on-line monitoring of secreted green fluorescence protein fusion proteins in Pichia pastor

Surribas, Anna; Resina, David; Ferrer, Pau et al.

Transcriptional analysis of protein production and induction of unfolded protein response in Pichia pastoris expressing

David Resina; M. Bollók; F. Valero et al.

Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

Gasser, Brigitte; Saloheimo, Markku; Rinas, Ursula et al.

Engineering and Applications of fungal laccases for organic synthesis

Kunamneni, Adinarayana; Camarero, Susana; García-Burgos, Carlos et al.

Substrate feeding strategies in Pichia pastoris fed-batch cultivation processes: Analysis of key parameters influencing

Ramón, Ramón; Cós, Oriol; Ferrer, Pau et al.

Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris

Mattanovich, Diethard; Graf, Alexandra B.; Stadlmann, Johannes et al.

Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris

Hans Marx; D. Mattanovich; M. Sauer

Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger β-galactosidase

Rodríguez, Ángel Pereira; Fernández-Leiro, Rafael; Trillo, M Cristina et al.

Macromolecular and elemental composition analysis and extracellular metabolite balances of Pichia pastoris growing at di

Carnicer, Marc; Baumann, Kristin; Töplitz, Isabelle et al.

Altering the ribosomal subunit ratio in yeast maximizes recombinant protein yield

Bonander, Nicklas; Darby, Richard A. J.; Grgic, Ljuban et al.

Transcriptional response of P. pastoris in fed-batch cultivations to Rhizopus oryzae lipase production reveals UPR induc

Resina, David; Bollók, Mónika; Khatri, Narendar K et al.

Microbial factories for recombinant pharmaceuticals

Ferrer‐Miralles, Neus; Domingo‐Espín, Joan; Corchero, José Luís et al.

Yeast cell factory: fishing for the best one or engineering it?

Porro, Danilo; Branduardi, Paola

Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway

Krainer, Florian; Dietzsch, Christian; Hajek, Tanja et al.

Open access to sequence: Browsing the Pichia pastoris genome

Mattanovich, Diethard; Callewaert, Nico; Rouzé, Pierre et al.

Tuning microbial hosts for membrane protein production

Freigaßner, Maria Elisabeth; Pichler, Harald; Glieder, Anton

The HAC1 gene from Pichia pastoris: characterization and effect of its overexpression on the production of secreted, sur

Guerfal, Mouna; Ryckaert, Stefan; Jacobs, Pieter P. et al.

Stepwise engineering of a Pichia pastoris D-amino acid oxidase whole cell catalyst

Abad, Sandra; Nahálka, Jozef; Bergler, Gabriele et al.

Recombinant norovirus-specific scFv inhibit virus-like particle binding to cellular ligands

K. Ettayebi; M. Hardy

Expression of an extremely acidic β-1,4-glucanase from thermoacidophilic Alicyclobacillus sp. A4 in Pichia pastoris is i

Bai, Yingguo; Wang, Jian‐She; Zhang, Zhifang et al.

High yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromo

Chen, Ruidong; Zhou, Zhigang; Cao, Yanan et al.

Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvem

Bevan Ks Chung; Suresh Selvarasu; Camattari Andrea et al.

High yield expression of leptospirosis vaccine candidates LigA and LipL32 in the methylotrophic yeast Pichia pastoris

Hartwig, Daiane Drawanz; Oliveira, Thaís Larré; Seixas, Fabiana K. et al.

Engineering Yarrowia lipolytica to Produce Glycoproteins Homogeneously Modified with the Universal Man3GlcNAc2 N-Glycan

Pourcq, Karen De; Tiels, Petra; Hecke, Annelies Van et al.

Codon Optimization Significantly Improves the Expression Level of a Keratinase Gene in Pichia pastoris

Hu, Hong; Gao, Jie; He, Jun et al.

Recombinant Expression of Margatoxin and Agitoxin-2 in Pichia pastoris: An Efficient Method for Production of KV1.3 Chan

Anangi, Raveendra; Koshy, Shyny; Huq, Redwan et al.

A Dual-Mode Surface Display System for the Maturation and Production of Monoclonal Antibodies in Glyco-Engineered Pichia

Shaheen, Hussam; Prinz, Bianka; Chen, Ming‐Tang et al.

β-Glucan Synthase Gene Overexpression and β-Glucans Overproduction in Pleurotus ostreatus Using Promoter Swapping

Chai, Ran; Qiu, Cuiwei; DongRen, Liu et al.

de novo Design and Synthesis of Candida antarctica Lipase B Gene and α-Factor Leads to High-Level Expression in Pichia p

Yang, Jiangke; Liu, Liying; Dai, Jianghong et al.

Conformational and Thermal Stability Improvements for the Large-Scale Production of Yeast-Derived Rabbit Hemorrhagic Dis

Fernández, Erlinda; Toledo, Jorge R.; Méndez, Lídice et al.

Characterization of the Pichia pastoris Protein-O-mannosyltransferase Gene Family

Nett, Juergen H.; Cook, W. James; Mingtang, Chen et al.

Enhanced Production of Recombinant Secretory Proteins in Pichia pastoris by Optimizing Kex2 P1’ site

Song, Yang; Kuang, Ye; Li, Hongbo et al.

Rational Design of a Fibroblast Growth Factor 21-Based Clinical Candidate, LY2405319

Kharitonenkov, Alexei; Beals, John M.; Micanovic, Radmila et al.

Engineering the Expression and Characterization of Two Novel Laccase Isoenzymes from Coprinus comatus in Pichia pastoris

Chunjuan, Gu; Zheng, Fei; Long, Liangkun et al.

Fusing a Carbohydrate-Binding Module into the Aspergillus usamii β-Mannanase to Improve Its Thermostability and Cellulos

Tang, Cunduo; Li, Jianfang; Wei, Xi-Huan et al.

Expression and Purification of Integral Membrane Fatty Acid Desaturases

Chen, Haiqin; Gu, Zhennan; Zhang, Hao et al.

The pCri System: A Vector Collection for Recombinant Protein Expression and Purification

Goulas, Theodoros; Cuppari, Anna; García-Castellanos, Raquel et al.

Structural and Functional Roles of Glycosylation in Fungal Laccase from Lentinus sp.

Maestre‐Reyna, Manuel; Liu, Wei-Chun; Jeng, W.Y. et al.

High-Level Expression of Endo-β-N-Acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and Its Applicat

Wang, Fei; Wang, Xiaojuan; Yu, Xiaolan et al.

Translational Arrest Due to Cytoplasmic Redox Stress Delays Adaptation to Growth on Methanol and Heterologous Protein Ex

Bryn Edwards-Jones; Rochelle Aw; G. Barton et al.

Overexpression of a Novel Thermostable and Chloride-Tolerant Laccase from Thermus thermophilus SG0.5JP17-16 in Pichia pa

Liu, Huiping; Cheng, Yu; Du, Bing et al.

A Highly Efficient Recombinant Laccase from the Yeast Yarrowia lipolytica and Its Application in the Hydrolysis of Bioma

Kalyani, Dayanand C.; Tiwari, Manish Kumar; Li, Jinglin et al.

Contribution of Disulfide Bridges to the Thermostability of a Type A Feruloyl Esterase from Aspergillus usamii

Yin, Xin; Hu, Die; Li, Jianfang et al.

Identification and Functional Characterization of Glycosylation of Recombinant Human Platelet-Derived Growth Factor-BB i

Dai, Mengmeng; Yu, Changming; Fang, Ting et al.

Computational Analysis and Low-Scale Constitutive Expression of Laccases Synthetic Genes GlLCC1 from Ganoderma lucidum a

Rivera-Hoyos, Claudia M.; Morales-Álvarez, Edwin D.; Cuevas, Sergio Alejandro Po

Relieving the first bottleneck in the drug discovery pipeline: using array technologies to rationalize membrane protein

Bonander, Nicklas; Bill, Roslyn M.

Expression and secretion of a biologically active glycoprotein hormone, ovine follicle stimulating hormone, by Pichia pa

Fidler, AE; Lun, S.; Young, Wayne et al.

Recombinants proteins for industrial uses: utilization of Pichia pastoris expression system

Rabert, Claudia; Weinacker, Daniel; Pessoa, Adalberto et al.

Expression and analysis of the glycosylation properties of recombinant human erythropoietin expressed in Pichia pastoris

Teh, Ser Huy; Fong, Mun Yik; Mohamed, Zulqarnain

Expression, purification and characterization of a phyA m -phyCs fusion phytase

Zou, Likou; Wang, Hongning; Pan, Xin et al.

Applications of recombinant Pichia pastoris in the healthcare industry

Weinacker, Daniel; Rabert, Claudia; Zepeda, Andrea et al.

ON-LINE MONITORING OF BIOMASS CONCENTRATION BASED ON A CAPACITANCE SENSOR: ASSESSING THE METHODOLOGY FOR DIFFERENT BACTE

Horta, Antônio Carlos Luperni; Silva, Adilson José da; Sargo, Cíntia Regina et a

Production of biologically active tethered ovine FSHbetaalpha by the methylotrophic yeast Pichia pastoris

Fidler, AE; Lin, Jian‐Sheng; Lun, S. et al.

Production systems for recombinant antibodies

Schirrmann, Thomas

Expression of codon optimized genes in microbial systems: current industrial applications and perspectives

Elena, Claudia; Ravasi, Pablo; Castelli, María Eugenia et al.

Recombinant protein expression in microbial systems

Rosano, Germán L.; Ceccarelli, Eduardo A.

Playing catch-up with Escherichia coli: using yeast to increase success rates in recombinant protein production experime

Bill, Roslyn M.

Recombinant protein production facility for fungal biomass-degrading enzymes using the yeast Pichia pastoris

Haon, Mireille; Grisel, Sacha; Navarro, David et al.

A comparative summary of expression systems for the recombinant production of galactose oxidase

Spadiut, Oliver; Olsson, Lisbeth; Brumer, Harry

Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Jacobs, Pieter P.; İnan, Mehmet; Festjens, Nele et al.

A multi-level study of recombinant Pichia pastoris in different oxygen conditions

Baumann, Kristin; Carnicer, Marc; Dragosits, Martin et al.

High yield recombinant thermostable α-amylase production using an improved Bacillus licheniformis system

Niu, Dandan; Zhirui, Zuo; Shi, Guiyang et al.

Cloning of the Zygosaccharomyces bailii GAS 1 homologue and effect of cell wall engineering on protein secretory phenoty

Passolunghi, Simone; Riboldi, Luca; Dato, Laura et al.

Heterologous expression of glucose oxidase in the yeast Kluyveromyces marxianus

Rocha, Saul Nitsche; Abrahão-Neto, José; Cerdán, M. Esperanza et al.

Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conve

Huang, Jinjin; Xia, Ji; Yang, Zhen et al.

Computational codon optimization of synthetic gene for protein expression

Chung, Bevan Kai-Sheng; Lee, Dong‐Yup

High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel production

Yu, Xiao‐Wei; Sha, Chong; Guo, Yong-Liang et al.

Yeast expressed recombinant Hemagglutinin protein of Novel H1N1 elicits neutralising antibodies in rabbits and mice

Athmaram, TN; Saraswat, Shweta; Santhosh, SR et al.

Glucose-methanol co-utilization in Pichia pastoris studied by metabolomics and instationary 13C flux analysis

Jordà, Joel; Suarez-Mendez, Camilo; Carnicer, Marc et al.

Development of a novel rDNA based plasmid for enhanced cell surface display on Yarrowia lipolytica

Bulani, Siyavuya Ishmael; Moleleki, Lucy; Albertyn, Jacobus et al.

High level secretion of cellobiohydrolases by Saccharomyces cerevisiae

Ilmén, Marja; Haan, Riaan den; Brevnova, Elena E. et al.

Implications of cellobiohydrolase glycosylation for use in biomass conversion

Jeoh, Tina; Michener, William E.; Himmel, Michael E. et al.

Construction of a novel Pichia pastoris strain for production of xanthophylls

Araya-Garay, José Miguel; Ageitos, José Manuel; Vallejo, Juán A. et al.

Genome-scale metabolic reconstructions of Pichia stipitis and Pichia pastoris and in silico evaluation of their potentia

Caspeta, Luis; Shoaie, Saeed; Ågren, Rasmus et al.

Integrated lipase production and in situ biodiesel synthesis in a recombinant Pichia pastoris yeast: an efficient dual b

Yan, Jinyong; Li, Zhaofeng; Du, Lei et al.

A new acidophilic thermostable endo-1,4-β-mannanase from Penicillium oxalicum GZ-2: cloning, characterization and functi

Liao, Hanpeng; Li, Shuixian; Zheng, Haiping et al.

Cloning and expression of Aspergillus flavus urate oxidase in Pichia pastoris

Fazel, Ramin; Zarei, Najmeh; Ghaemi, Nasser et al.

Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris

Cheng Li; Ying-Wu Lin; Xueyun Zheng et al.

Functional recombinant protein is present in the pre-induction phases of Pichia pastoris cultures when grown in bioreact

Bawa, Zharain; Routledge, Sarah J; Jamshad, Mohammed et al.

Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

Rüth, Claudia; Buchetics, Markus; Vidimce, Viktorija et al.

Secretion of a foreign protein from budding yeasts is enhanced by cotranslational translocation and by suppression of va

Fitzgerald, Ivy; Glick, Benjamin S.

Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus

Yarimizu, Tohru; Nakamura, Mikiko; Hoshida, Hisashi et al.

High-temperature cultivation of recombinant Pichia pastorisincreases endoplasmic reticulum stress and decreases producti

Zhong, Yongjun; Yang, Lu; Guo, Yugang et al.

Role of N-linked glycosylation in the secretion and enzymatic properties of Rhizopus chinensis lipase expressed in Pichi

Yang, Min; Yu, Xiao‐Wei; Zheng, Haiyan et al.

Systematic comparison of single-chain Fv antibody-fusion toxin constructs containing Pseudomonas Exotoxin A or saporin p

Cristina, Pietro Della; Castagna, Monica; Lombardi, Alessio et al.

Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase

Gmeiner, Christoph; Saadati, Amirhossein; Maresch, Daniel et al.

Construction of efficient xylose utilizing Pichia pastoris for industrial enzyme production

Pengfei Li; Hongbing Sun; Zao Chen et al.

Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris

Vogl, Thomas; Ahmad, Mudassar; Krainer, Florian et al.

Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris

Florian W. Krainer; Simona Capone; Martin Jäger et al.

Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specif

Marco, Ario de

ATP regulation in bioproduction

Hara, Kiyotaka Y.; Kondo, Akihiko

Development of a mixed feed strategy for a recombinant Pichia pastoris strain producing with a de-repression promoter

Capone, Simona; Horvat, Jernej; Herwig, Christoph et al.

Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology

Brigitte Gasser; Matthias G. Steiger; D. Mattanovich

The sweet branch of metabolic engineering: cherry-picking the low-hanging sugary fruits

Chen, Rachel

Expression of MTHase Gene in Pichia pastoris and Its Genetic Stability

Li, Dehua

Engineering the Pattern of Protein Glycosylation Modulates the Thermostability of a GH11 Xylanase

Fonseca-Maldonado, Raquel; Vieira, Davi Serradella; Alponti, Juliana Sanchez et

Structural Analysis of a Glycoside Hydrolase Family 11 Xylanase from Neocallimastix patriciarum

Cheng, Ya‐Shan; Chen, Chun‐Chi; Huang, Chun‐Hsiang et al.

The importance of connections between the cell wall integrity pathway and the unfolded protein response in filamentous f

Malavazi, Iran; Goldman, Gustavo H.; Brown, Neil

Specific Characterization of Substrate and Inhibitor Binding Sites of a Glycosyl Hydrolase Family 11 Xylanase fromAsperg

Tahir, Tariq A.; Berrin, Jean‐Guy; Flatman, Ruth H. et al.

A Fast-acting, Modular-structured Staphylokinase Fusion with Kringle-1 from Human Plasminogen as the Fibrin-targeting Do

Wu, Sau‐Ching; Castellino, Francis; Wong, Sui‐Lam

Glycosylation Increases the Thermostability of Human Aquaporin 10 Protein

Öberg, Fredrik; Sjöhamn, Jennie; Fischer, Gerhard W. et al.

Efforts to make and apply humanized yeast

Laurent, Jon M.; Young, Jonathan H.; Kachroo, Aashiq H. et al.

Towards a better understanding of the generation of fructan structure diversity in plants: molecular and functional char

Lasseur, Bertrand; Lothier, J.; Wiemken, Andres et al.

Glyco-variant library of the versatile enzyme horseradish peroxidase

Simona Capone; Robert Pletzenauer; D. Maresch et al.

Differences in enzymic properties of five recombinant xyloglucan endotransglucosylase/hydrolase (XTH) proteins of Arabid

Maris, A.; Kaewthai, Nomchit; Eklöf, Jens et al.

Rational engineering of the fungal P450 monooxygenase CYP5136A3 to improve its oxidizing activity toward polycyclic arom

Syed, Khajamohiddin; Porollo, Aleksey; Miller, Dwight W. et al.

Blocking the tunnel: engineering of Candida rugosa lipase mutants with short chain length specificity

Schmitt, Jutta; Brocca, Stefania; Schmid, Rolf D. et al.

Biased mutagenesis in the N-terminal region by degenerate oligonucleotide gene shuffling enhances secretory expression o

Fukuda, Kenji; Jensen, Malene H.; Haser, Richard et al.

Cloning and functional analysis of a fructosyltransferase cDNA for synthesis of highly polymerized levans in timothy (Ph

Tamura, Ken‐ichi; Kawakami, Akira; Sanada, Yoshinori et al.

Single-Chain Fv with Fc Fragment of the Human IgG1 Tag: Construction, Pichia pastoris Expression and Antigen Binding Cha

Andrade, Edmar Vaz de; Albuquerque, Flávia Caixeta; Moraes, Lídia Maria Pepe de

Promoter library designed for fine-tuned gene expression in Pichia pastoris

F. Hartner; C. Ruth; David Langenegger et al.

Improving industrial yeast strains: exploiting natural and artificial diversity

Steensels, Jan; Snoek, Tim; Meersman, Esther et al.

Characterization of<i>LeMir</i>, a Root-Knot Nematode-Induced Gene in Tomato with an Encoded Product Secreted from the R

Brenner, Eric D.; Lambert, Kris N.; Kaloshian, Isgouhi et al.

The secretory pathway: exploring yeast diversity

Delic, Marizela; Valli, Minoska; Graf, Alexandra B. et al.

Improved pectinase production in Penicillium griseoroseum recombinant strains

Teixeira, J. A.; Gonçalves, Daniel Bonoto; Queiroz, Marisa Vieira de et al.

<i>NmDef02</i>, a novel antimicrobial gene isolated from <i>Nicotiana megalosiphon</i> confers high‐level pathogen resis

Portieles, Roxana; Ayra‐Pardo, Camilo; González, Ernesto et al.

Cell-surface phytase on Pichia pastoris cell wall offers great potential as a feed supplement

Harnpicharnchai, Piyanun; Sornlake, Warasirin; Tang, Kittapong et al.

Transcriptomics-Based Identification of Novel Factors Enhancing Heterologous Protein Secretion in Yeasts

Brigitte Gasser; M. Sauer; Michael Maurer et al.

Recombinant protein subunit vaccine synthesis in microbes: a role for yeast?

Bill, Roslyn M.

Impact of the native‐state stability of human lysozyme variants on protein secretion by <i>Pichia pastoris</i>

Kumita, Janet R.; Johnson, Russell J.; Alcocer, Marcos et al.

Humanization of Yeast to Produce Complex Terminally Sialylated Glycoproteins

Hamilton, Stephen R.; Davidson, Robert C.; Sethuraman, Natarajan et al.

Overexpression of an anti-CD3 immunotoxin increases expression and secretion of molecular chaperone BiP/Kar2p by Pichia

Liu, Yuan Yi; Woo, Jung Hee; Neville, David M

Combinatorial Mutagenesis and Selection to Understand and Improve Yeast Promoters

Berg, Laila; Strand, Trine Aakvik; Valla, Svein et al.

Extending the Serum Half-Life of G-CSF via Fusion with the Domain III of Human Serum Albumin

Zhao, Shuqiang; Zhang, Yu; Tian, Hong et al.

Recombinant Expression of Pleurocidin cDNA Usingthe Pichia pastoris Expression System

Burrowes, Olive-Jean; Diamond, Gill; Lee, Tung-Ching

Codon Optimization Significantly Improves the Expression Level of<i>α</i>-Amylase Gene from<i>Bacillus licheniformis</i>

Wang, Jian‐Rong; Li, Yang-Yuan; Liu, Dan-Ni et al.

Expression and Characterization of<i>Geobacillus stearothermophilus</i>SR74 Recombinant<i>α</i>-Amylase in<i>Pichia past

Gandhi, S. R.; Salleh, Abu Bakar; Rahman, Raja Noor Zaliha Raja Abd et al.

Comparative Evaluation of Recombinant Protein Production in Different Biofactories: The Green Perspective

Merlin, Matilde; Gecchele, Elisa; Capaldi, Stefano et al.

Comprehensive structural annotation of Pichia pastoris transcriptome and the response to various carbon sources using de

Liang, Shuli; Wang, Bin; Li, Pan et al.

Cloning and Expression of Synthetic Genes Encoding the Broad Antimicrobial Spectrum Bacteriocins SRCAM 602, OR-7, E-760,

Arbulu, Sara; Jiménez, Juan J.; Gútiez, Loreto et al.

Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays

A. Graf; Brigitte Gasser; Martin Dragosits et al.

Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast <i>Gla

Alias, Norsyuhada; Mazian, Muadz Ahmad; Salleh, Abu Bakar et al.

Genome sequence and analysis of methylotrophic yeast Hansenula polymorpha DL1

Ravin, Nikolai V.; Eldarov, Michael A.; Kadnikov, Vitaly V. et al.

Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions

Brigitte Gasser; Michael Maurer; J. Rautio et al.

Effects of biodiesel waste glycerol on the growth characteristics of Pichia pastoris genetically modified to produce spi

Cui, Xuemei; Ellison, Michael S.

GAP promoter library for fine-tuning 1 gene expression in Pichia pastoris 2 3 Running title : GAP promoter library for f

Xiulin Qin; Jiangchao Qian; Gaofeng Yao et al.

F gene and HN gene co-express of Newcastle disease virus strain F48E9 in Pichia pastoris

Luo-Dan Cheng

[Enhancement of Coprinus cinereus peroxidase in Pichia pastoris by co-expression chaperone PDI and Ero1].

Chen, Fei; Hu, Meirong; Jiang, Xianzhang et al.

Microbial Cell Factories Recombinant Production of Bacterial Toxins and Their Derivatives in the Methylotrophic Yeast Pi

C. Gurkan; D. Ellar; Email et al.

Expression of the Rhizopus oryzae lipase in Pichia pastoris under the control of the FLD1 promoter

D. Rodriguez

[High-level expression of the potential vaccine antigen TSO18 of Taenia solium in Pichia pastoris].

Yuan, Gailing; Cai, Xue‐Peng; Jing, Zhizhong et al.

[Secretory expression of recombinant porcine zona pellucida glycoprotein-3alpha (rpZP3alpha) in Pichia pastoris].

Gao, Yan-ru; Shan-pei, Pan; Xie, Qi-Xuan et al.

Secreted Expression of S-adenosy-L-methionine Synthetase in Pichia pastoris

He, Guangyuan

Purification,Identification and Optimized Expression of Recombinant Human Neuritin in Pichia Pastoris

Huang, Jinbao

STRATEGY DESIGN FOR THERAPEUTICAL PROTEIN PRODUCTION by Pichia pastoris

P. Çalık

Pichia pastoris as a cell factory for the secreted production of tunable collagen-inspired gel-forming proteins

Cicera Elaine da Silva

Metabolic studies on Schizosaccharomyces pombe for improved protein secretion

Tobias Klein

Recombinant Lipases and Phospholipases and Their Use as Biocatalysts for Industrial Applications

Borrelli, Grazia Maria; Trono, Daniela

High-throughput screening and selection of yeast cell lines expressing monoclonal antibodies

Barnard, Gavin C.; Kull, Angela; Sharkey, Nathan S. et al.

Introduction of a disulfide bridge enhances the thermostability of a Streptomyces olivaceoviridis xylanase mutant

Yang, Hong; Yao, Bin; Meng, Kun et al.

A simple<i>Pichia pastoris</i>fermentation and downstream processing strategy for making recombinant pandemic Swine Orig

Athmaram, T. N.; Singh, Ajay; Saraswat, Shweta et al.

Constitutive expression of human angiostatin in Pichia pastoris by high-density cell culture

Zhang, A. L.; Zhang, T. Y.; Luo, J. X. et al.

Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement

Zhang, Wenhui; Potter, Karen J.; Plantz, Bradley A. et al.

Current state and recent advances in biopharmaceutical production in Escherichia coli, yeasts and mammalian cells

A. Berlec; B. Štrukelj

Scale-up fermentation of recombinant Candida rugosa lipase expressed in Pichia pastoris using the GAP promoter

Zhao, Wei; Wang, Jinwen; Deng, Riqiang et al.

Directed modification of the <i>Aspergillus usamii</i> β-mannanase to improve its substrate affinity by in silico design

Li, Jianfang; Wei, Xi-Huan; Tang, Cunduo et al.

Optimization of cell density and dilution rate in Pichia pastoris continuous fermentations for production of recombinant

Zhang, Wenhui; Liu, Chih-Ping; İnan, Mehmet et al.

Functional expression of trypsin from Streptomyces griseus by Pichia pastoris

Ling, Zhenmin; Ma, Tengbo; Li, Jianghua et al.

Efficient secretion of lipase r27RCL in <i>Pichia pastoris</i> by enhancing the disulfide bond formation pathway in the

Sha, Chong; Yu, Xiao‐Wei; Zhang, Meng et al.

Disruption of <i>YPS1</i> and <i>PEP4</i> genes reduces proteolytic degradation of secreted HSA/PTH in <i>Pichia pastori

Wu, Min; Shen, Qi; Yang, Yong et al.

Enhanced production of <i>Thermomyces lanuginosus</i> lipase in <i>Pichia pastoris</i> via genetic and fermentation stra

Fang, Zhonggang; Xu, Li; Pan, Dujie et al.

Enhanced expression of endoinulinase from <i>Aspergillus niger</i> by codon optimization in <i>Pichia pastoris</i> and i

He, Miao; Wu, Dan; Wu, Jing et al.

Engineering the supply chain for protein production/secretion in yeasts and mammalian cells

Klein, Tobias; Niklas, Jens; Heinzle, Elmar

Combined use of <i>GAP</i> and <i>AOX1</i> promoters and optimization of culture conditions to enhance expression of <i>

Dong, He; Luo, Wen; Wang, Zhiyuan et al.

Improving Enzymes for Biomass Conversion: A Basic Research Perspective

Banerjee, Goutami; Scott‐Craig, John S.; Walton, Jonathan D.

Assay and heterologous expression in Pichia pastoris of plant cell wall type-II membrane anchored glycosyltransferases

Petersen, Bent Larsen; Egelund, Jack; Damager, Iben et al.

New yeast expression platforms based on methylotrophic and and on dimorphic and – A comparison

Gellissen, Gerd; Kunze, Gotthard; Gaillardin, C. et al.

Overexpression of the Neocallimastix frontalis xylanase gene in the methylotrophic yeasts Pichia pastoris and Pichia met

Tsai, Chang Ting; Huang, Ching‐Tsan

Cloning and characterization of the gene as a selectable marker

Thor, Der; Xiong, Sidong; Orazem, Claire C. et al.

Neutral protease expression and optimized conditions for the degradation of blood cells using recombinant Pichia pastori

Hao, Zhang; Zhang, Boru; Zheng, Yanbin et al.

Reliable high-throughput screening with by limiting yeast cell death phenomena

Weis, Robert; Luiten, Ruud; Skranc, Wolfgang et al.

Expression and characterization of Gag protein of HIV-1CN in Pichia pastoris

Jiang, Wen Zhi; Jin, Ning; Li, Zi J. et al.

Intracellular expression and purification of the Canstatin-N protein in Pichia pastoris

Yin, Huixiang; Liu, Zhenwang; Zhang, Ailian et al.

Effect of molecular chaperones on the expression of Candida antarctica lipase B in Pichia pastoris.

Samuel, Premsingh; Prasanna Vadhana, Ashok Kumar; Kamatchi, Ramakrishnan et al.

Over expression of anti-MUC1 single-domain antibody fragments in the yeast Pichia pastoris

Rahbarizadeh, Fatemeh; Rasaee, Mohammad Javad; Forouzandeh, Mehdi et al.

Engineering of bottlenecks in Rhizopus oryzae lipase production in Pichia pastoris using the nitrogen source-regulated F

David Resina; Michael Maurer; O. Cós et al.

Towards improved membrane protein production in Pichia pastoris: general and specific transcriptional response to membra

Vogl, Thomas; Thallinger, Gerhard G; Zellnig, Guenther et al.

Substrate specificity and regioselectivity of fungal AA9 lytic polysaccharide monooxygenases secreted by Podospora anser

Bennati-Granier, Chloé; Garajová, Soňa; Champion, Charlotte et al.

A constitutive expression system for glycosyl hydrolase family 7 cellobiohydrolases in Hypocrea jecorina

Linger, Jeffrey; Taylor, Larry E.; Baker, John O. et al.

Engineering towards a complete heterologous cellulase secretome in Yarrowia lipolytica reveals its potential for consoli

Wei, Hui; Wang, Wei; Alahuhta, Markus et al.

Production of medium-chain volatile flavour esters in Pichia pastoris whole-cell biocatalysts with extracellular express

Zhuang, Shiwen; Fu, Junshu; Powell, Chris et al.

High-Level Expression of Fusion Protein Containing 10 Tandem Repeated GLP-1 Analogs in Yeast<i>Pichia pastoris</i>and It

Hou, Jianhua; Yan, Ruixiang; Yang, Liquan et al.

Stabilisation of the Fc Fragment of Human IgG1 by Engineered Intradomain Disulfide Bonds

Wozniak‐Knopp, Gordana; Stadlmann, Johannes; Rüker, Florian

Secretory expression of thermostable alkaline protease from <i>Bacillus stearothermophilus</i> FI by using native signal

Latiffi, Amaliawati Ahmad; Salleh, Abu Bakar; Rahman, Raja Noor Zaliha Raja Abd

A Gene Optimization Strategy that Enhances Production of Fully Functional P-Glycoprotein in Pichia pastoris

Bai, Jiangping; Swartz, Douglas J.; Protasevich, Irina I. et al.

Native-State Stability Determines the Extent of Degradation Relative to Secretion of Protein Variants from Pichia pastor

G. Whyteside; M. Alcocer; J. Kumita et al.

Systematic single-cell analysis of Pichia pastoris reveals secretory capacity limits productivity.

Love, Kerry Routenberg; Politano, Timothy J; Panagiotou, Vasiliki et al.

Amino Acid Substitutions at the Major Insertion Loop of Candida albicans Sterol 14alpha-Demethylase Are Involved in Fluc

Alvarez-Rueda, Nidia; Fleury, Audrey; Morio, Florent et al.

Use of HDEL‐tagged Trichoderma reesei mannosyl oligosaccharide 1,2‐α‐ D ‐mannosidase for N ‐glycan engineering in Pichia

Callewaert, Nico; Laroy, Wouter; Cadirgi, Hasan et al.

Recombinant Batroxobin Expressed Highly in Pichia pastoris

Li, Zhi; Yu, Xijie; Huang, Jinling et al.

Heterologous protein expression in the methylotrophic yeast Pichia pastoris

Cereghino, Joan Lin

Enhanced secretion of heterologous proteins in Pichia pastoris following overexpression of Saccharomyces cerevisiae chap

Zhang, Wei; Zhao, Hong-Liang; Xue, Chong et al.

Synthetic Core Promoters for Pichia pastoris

T. Vogl; C. Ruth; Julia Pitzer et al.

Genome sequence of the recombinant protein production host Pichia pastoris

Schutter, Kristof De; Lin, Yao‐Cheng; Tiels, Petra et al.

High-yield novel leech hyaluronidase to expedite the preparation of specific hyaluronan oligomers

Jin, Peng; Kang, Zhen; Zhang, Na et al.

Codon optimisation improves the expression of Trichoderma viride sp. endochitinase in Pichia pastoris

Yu, Ping; Yan, Yuan; Gu, Qing et al.

An Efficient Genome-Wide Fusion Partner Screening System for Secretion of Recombinant Proteins in Yeast

Bae, Jung-Hoon; Sung, Bong Hyun; Kim, Hyun‐Jin et al.

Knockout of an endogenous mannosyltransferase increases the homogeneity of glycoproteins produced in Pichia pastoris

Krainer, Florian; Gmeiner, Christoph; Neutsch, Lukas et al.

Functional diversity and properties of multiple xylanases from Penicillium oxalicum GZ-2

Liao, Hanpeng; Zheng, Haiping; Li, Shuixian et al.

Enhanced and homogeneous oxygen availability during incubation of microfluidic droplets

Mahler, Lisa; Tovar, Miguel; Weber, Thomas et al.

Glycosylation by Pichia pastoris decreases the affinity of a family 2a carbohydrate-binding module from Cellulomonas fim

Boraston, A.B.; Warren, R. Antony J.; Kilburn, Douglas G.

The C-terminal segment of the 1,3-beta-glucanase Ole e 9 from olive (Olea europaea) pollen is an independent domain with

Palomares, Óscar; Villalba, Mayte; Rodrı́guez, Rosalı́a

Multiple mutagenesis of non-universal serine codons of the Candida rugosa LIP2 gene and biochemical characterization of

LEE, Guan-Chiun; Lee, Li-Chiun; Sava, Vasyl et al.

Substrate (aglycone) specificity of human cytosolic beta-glucosidase

Berrin, Jean‐Guy; Czjzek, Mirjam; Kroon, Paul A. et al.

Propeptide dependent activation of the Antarctic krill euphauserase precursor produced in yeast

Kristjánsdóttir, Sigríður; Guðmundsdóttir, Ágústa

Characterization of potato proteinase inhibitor II reactive site mutants

Beekwilder, Jules; Schipper, Bert; Bakker, Petra L. et al.

Ktr1p is an α-1,2-mannosyltransferase of Saccharomyces cerevisiae. Comparison of the enzymic properties of soluble recom

Romero, Pedro; Lussier, Marc; Sdicu, Anne‐Marie et al.

Procarboxypeptidase A from the insect pest Helicoverpa armigera and its derived enzyme

Bayés, Àlex; Sonnenschein, Anka; Daura, Xavier et al.

Role of the Occluding Loop in Cathepsin B Activity

Illy, Chantal; Quraishi, Omar; Wang, Jing et al.

Structural analysis of the CD5 antigen

McAlister, M.; Brown, Marion H.; Willis, Antony C. et al.

Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast<i>Pichia</i><i>pastoris</i>

Choi, Byung‐Kwon; Bobrowicz, Piotr; Davidson, Robert C. et al.

In Mouse α-Methylacyl-CoA Racemase, the Same Gene Product Is Simultaneously Located in Mitochondria and Peroxisomes

Kotti, Tiina; Savolainen, Kalle; Helander, Heli et al.

Pichia pastoris-expressed dengue 3 envelope-based virus-like particles elicit predominantly domain III-focused high tite

Tripathi, Lav; Mani, Shailendra; Raut, Rajendra et al.

Arming Technology in Yeast—Novel Strategy for Whole-cell Biocatalyst and Protein Engineering

Kuroda, Kouichi; Ueda, Mitsuyoshi

Combining Protein and Strain Engineering for the Production of Glyco-Engineered Horseradish Peroxidase C1A in Pichia pas

Capone, Simona; Ćorajević, Lejla; Bonifert, Günther et al.

Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris

Wu, Jyh-Ming; Wang, Shin-Yao; Fu, Wei-Chang

Isolation and Characterization of the Diatom Phaeodactylum Δ5-Elongase Gene for Transgenic LC-PUFA Production in Pichia

Jiang, Mulan; Guo, Bing; Wan, Xia et al.

Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production

Jordà, Joel; Rojas, Hugo; Carnicer, Marc et al.

Secretion and activity of antimicrobial peptide cecropin D expressed in Pichia pastoris

Guo, Chunhe; Huang, Yumao; Zheng, Hongyu et al.

Direct evaluation of the effect of gene dosage on secretion of protein from yeast Pichia pastoris by expressing EGFP.

Hailong Liu; Yufeng Qin; Yuankai Huang et al.

Design, construction, and in vitro analysis of A33scFv::CDy, a recombinant fusion protein for antibody-directed enzyme p

Coelho, Vânia; Dernedde, Jens; Petrausch, Ulf et al.

Comparison of Two Laccases from Trametes versicolor for Application in the Decolorization of Dyes

Li, Qi; Ge, Lin; Cai, Junli et al.

Expression of porcine protegrin-1 in Pichia pastoris and its anticancer activity in vitro

Niu, Mingfu; Chai, Shumao; You, Xiaoyan et al.

Cholera Toxin Subunit B as Adjuvant––An Accelerator in Protective Immunity and a Break in Autoimmunity

Stratmann, Thomas

Fungal Laccases: Production, Function, and Applications in Food Processing

Brijwani, Khushal; Rigdon, Anne R.; Vadlani, Praveen V.

Secretion and expression dynamics of a GFP-tagged mucin-type fusion protein in high cell density Pichia pastoris bioreac

M. Sjöblom; L. Lindberg; J. Holgersson et al.

Constitutive expression of Botrytis aclada laccase in Pichia pastoris

R. Kittl; Christoph Gonaus; Christian Pillei et al.

Recombinant expression and characterization of an endoglucanase III (cel12a) from Trichoderma harzianum (Hypocreaceae) i

Generoso, Wesley Cardoso; Malagó-Jr, Wilson; Pereira, Nei et al.

[Burkholderia cepacia lipase gene modification and its constitutive and inducible expression in Pichia pastoris].

Bin Jia; Wenshan Liu; Jiangke Yang et al.

[Cloning of a laccase gene from Flammulina velutipes and study on its expression in Pichia pastoris].

Yin-Bo Zhang; Q. Jiang; Mulan Jiang et al.

Characterisation, cloning and production of industrially interesting enzymes : gluconolactone oxidase of Penicillium cya

Salusjärvi, Tuomas

Different approaches to make Pichia pastoris a more versatile expression platform – process, strain and product engineer

D. Mattanovich; O. Spadiut; B. Eggenreich et al.

Comparison of two codon optimization strategies enhancing recombinant Sus scrofa lysozyme production in Pichia pastoris.

D. Zhu; G. Cai; D. Wu et al.

An updated view on horseradish peroxidases: recombinant production and biotechnological applications

Krainer, Florian; Glieder, Anton

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

Mudassar Ahmad; Melanie Hirz; H. Pichler et al.

Filamentous fungi-like secretory pathway strayed in a yeast system: peculiarities of Yarrowia lipolytica secretory pathw

Celińska, Ewelina; Nicaud, Jean‐Marc

Pecularities and applications of aryl-alcohol oxidases from fungi

Urlacher, Vlada B.; Koschorreck, Katja

Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant pr

Raschmanová, Hana; Weninger, Astrid; Knejzlík, Zdeněk et al.

Carbon source dependent promoters in yeasts

Weinhandl, Katrin; Winkler, Margit; Glieder, Anton et al.

Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pas

Cós, Oriol; Ramón, Ramón; Montesinos, José Luis González et al.

Integration and Validation of the Genome-Scale Metabolic Models of Pichia pastoris: A Comprehensive Update of Protein Gl

Tomàs‐Gamisans, Màrius; Ferrer, Pau; Albiol, Joan

Recombinant gene expression : reviews and protocols

P. Balbás; A. Lorence

New Developments in Pichia pastoris Expression System, Review and Update.

Roghayyeh Baghban; S. Farajnia; Y. Ghasemi et al.

Production of enzymes by biotechnological methods: A review

A. Dub; Iuliia Plaskonis; Oksana Barna et al.

Fluorescent Reporter Libraries as Useful Tools for Optimizing Microbial Cell Factories: A Review of the Current Methods

Delvigne, Frank; Pêcheux, Hélène; Tarayre, Cédric

Book Review: Recent Advances in Yeast Metabolic Engineering

Gohil, Nisarg; Panchasara, Happy; Patel, Shreya K. et al.

Industrial Use of Cell Wall Degrading Enzymes: The Fine Line Between Production Strategy and Economic Feasibility

Giovannoni, Moira; Gramegna, Giovanna; Benedetti, Manuel et al.

Multiplex Genome Engineering Methods for Yeast Cell Factory Development

Malcı, Koray; Walls, Laura E.; Rios‐Solis, Leonardo

Identification and Expression of New Unspecific Peroxygenases – Recent Advances, Challenges and Opportunities

Kinner, Alina; Rosenthal, Katrin; Lütz, Stephan

Yeast Surface Display System: Strategies for Improvement and Biotechnological Applications

Teymennet‐Ramírez, Karla Verónica; Martínez‐Morales, Fernando; Trejo‐Hernández,

Cell factories for insulin production

Baeshen, Nabih A.; Baeshen, Mohammed N.; Sheikh, Abdullah et al.

Bioprospecting and biotechnological insights into sweet-tasting proteins by microbial hosts―a review

M. Bilal; Liyun Ji; Shuo Xu et al.

Yeast synthetic biology for designed cell factories producing secretory recombinant proteins

Thak, Eun Jung; Yoo, Su Jin; Moon, Hye Yun et al.

Editorial for the thematic tssue on &quot;Industrial Microbiology&quot;.

Colin R. Harwood; Sunghoon Park; Michael Sauer

Multiplex genome editing of microorganisms using CRISPR-Cas

Adiego-Pérez, Belén; Randazzo, Paola; Daran, Jean‐Marc et al.

A review of yeast: High cell-density culture, molecular mechanisms of stress response and tolerance during fermentation

Shen, Dongxu; He, Xiaoli; Weng, Peifang et al.

[Genetic engineering of Pichia pastoris expression system for improved secretion of heterologous proteins--a review].

Guan, Bo; Jin, Jian; Li, Huazhong

Recombinant gene expression : reviews and protocols

P. Balbás; A. Lorence

A detailed overview of xylanases: an emerging biomolecule for current and future prospective

Bhardwaj, Nisha; Kumar, Bikash; Verma, Pradeep

Current perspective on production and applications of microbial cellulases: a review

Bhardwaj, Nisha; Kumar, Bikash; Agrawal, Komal et al.

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Tripathi, Nagesh K.; Shrivastava, Ambuj

Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory

Miguel Angel Nieto-Taype; Xavier Garcia-Ortega; J. Albiol et al.

Improving Immunotherapy Through Glycodesign

Buettner, Matthew J.; Shah, Sagar; Saeui, Christopher T. et al.

Expression of Recombinant Antibodies

Frenzel, André; Hust, Michael; Schirrmann, Thomas

Technological Microbiology: Development and Applications

Vitorino, Luciana Cristina; Bessa, Layara Alexandre

Improvement Strategies, Cost Effective Production, and Potential Applications of Fungal Glucose Oxidase (GOD): Current U

Dubey, Manish Kumar; Zehra, Andleeb; Aamir, Mohd et al.

A Review of the Microbial Production of Bioactive Natural Products and Biologics

Pham, Janette V.; Yilma, Mariamawit A.; Feliz, Adriana et al.

A Review of Chondroitin Sulfate’s Preparation, Properties, Functions, and Applications

Shen, Qingshan; Guo, Yujie; Wang, Kangyu et al.

Aspergillus as a multi-purpose cell factory: current status and perspectives

Meyer, Vera; Wu, Bo; Ram, Arthur F. J.

A Systematic Review of the Potential of Pichia pastoris (Komagataella phaffii) as an Alternative Host for Biologics Prod

Vijay Elakkya Vijayakumar; Krishnan Venkataraman

Microbial xylanases and their industrial application in pulp and paper biobleaching: a review

Walia, Abhishek; Guleria, Shiwani; Mehta, Preeti et al.

Cultivation strategies to enhance productivity of Pichia pastoris: A review

Looser, Verena; Brühlmann, Béla; Bumbak, Fabian et al.

From small to large-scale: a review of recombinant spider silk and collagen bioproduction

Delgado‐Gomes, Vasco; Salgueiro, Sancha P.

Challenges and progress towards industrial recombinant protein production in yeasts: A review

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