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Host Cell Protein Clearance Across Multi Step Puri

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Monoclonal antibody purification using cationic polyelectrolytes: an alternative to column chromatography.

2010 Biotechnology progress

Peram, Thanmaya; McDonald, Paul; Carter-Franklin, Jayme et al.

DOI

Host cell protein clearance during protein A chromatography: development of an improved column wash step.

2008 Biotechnology progress

Shukla, Abhinav A; Hinckley, Peter

DOI

Protein Equalizer™ Technology : The quest for a “democratic proteome”

2006 PROTEOMICS

Righetti, Pier Giorgio; Boschetti, Egisto; Lomas, Lee et al.

DOI

Absolute quantitation of host cell proteins in recombinant human monoclonal antibodies with an automated CZE-ESI-MS/MS s

2014 Electrophoresis

Zhu, Guijie; Sun, Liangliang; Linkous, Travis et al.

DOI

Improved detection of host cell proteins (HCPs) in a mammalian cell-derived antibody drug using liquid chromatography/ma

2014 Rapid communications in mass spectrometry : RCM

Thompson, Jenny Heidbrink; Chung, Wai Keen; Zhu, Min et al.

DOI

Proteomic studies support the use of multi‐product immunoassays to monitor host cell protein impurities

2005 PROTEOMICS

Krawitz, Denise C.; Forrest, William F.; Moreno, G. Tony et al.

DOI

Analysis of host-cell proteins in biotherapeutic proteins by LC/MS approaches.

2014 Methods in molecular biology (Clifton, N.J.)

Doneanu, Catalin E; Chen, Weibin

DOI

2-D Western blotting for evaluation of antibodies developed for detection of host cell protein.

2015 Methods in molecular biology (Clifton, N.J.)

Berkelman, Tom; Harbers, Adriana; Bandhakavi, Sricharan

DOI

Host cell contaminant protein assay development for recombinant biopharmaceuticals.

1995 Journal of chromatography. A

Eaton, L C

DOI

Separation of product associating E. coli host cell proteins OppA and DppA from recombinant apolipoprotein A-I(Milano) i

2009 Biotechnology progress

Hunter, Alan K; Wang, Xing; Suda, Eric J et al.

DOI

Purification of proteins from solutions containing residual host cell proteins via preparative crystallization.

2015 Biotechnology letters

Hekmat, Dariusch; Breitschwerdt, Peter; Weuster-Botz, Dirk

DOI

A mass spectrometry-based approach to host cell protein identification and its application in a comparability exercise.

2014 Analytical biochemistry

Reisinger, Veronika; Toll, Hansjoerg; Mayer, Robert Ernst et al.

DOI

Single step plasmid DNA purification using methacrylate monolith bearing combination of ion-exchange and hydrophobic gro

2013 Journal of chromatography. A

Smrekar, Vida; Smrekar, Franc; Strancar, Aleš et al.

DOI

Proteomics-based, multivariate random forest method for prediction of protein separation behavior during cation-exchange

2012 Journal of chromatography. A

Swanson, Ryan K; Xu, Ruo; Nettleton, Dan et al.

DOI

Accelerated purification process development of monoclonal antibodies for shortening time to clinic. Design and case stu

2007 Journal of chromatography. A

Ishihara, Takashi; Kadoya, Toshihiko

DOI

Antibody capture by mixed-mode chromatography: a comprehensive study from determination of optimal purification conditio

2011 Journal of chromatography. A

Pezzini, Jerome; Joucla, Gilles; Gantier, René et al.

DOI

Purification process of recombinant monoclonal antibodies with mixed mode chromatography.

2015 Journal of chromatography. A

Maria, Sophie; Joucla, Gilles; Garbay, Bertrand et al.

DOI

Studying host cell protein interactions with monoclonal antibodies using high throughput protein A chromatography.

2012 Biotechnology journal

Sisodiya, Vikram N; Lequieu, Joshua; Rodriguez, Maricel et al.

DOI

Host cell protein analysis in therapeutic protein bioprocessing - methods and applications.

2013 Biotechnology journal

Tscheliessnig, Anne Luise; Konrath, Julita; Bates, Ron et al.

DOI

Generic chromatography-based purification strategies accelerate the development of downstream processes for biopharmaceu

2014 Biotechnology journal

Buyel, Johannes F; Fischer, Rainer

DOI

Continuous purification of antibodies from cell culture supernatant with aqueous two‐phase systems: From concept to proc

2012 Biotechnology Journal

Rosa, Paula A.J.; Azevedo, Ana M.; Sommerfeld, Sven D. et al.

DOI

High‐throughput screening of packed‐bed chromatography coupled with SELDI‐TOF MS analysis: monoclonal antibodies versus

2007 Biotechnology and Bioengineering

Wierling, P. Schulze; Bogumil, Ralf; Knieps‐Grünhagen, Esther et al.

DOI

Host cell proteins in biologics development: Identification, quantitation and risk assessment.

2009 Biotechnology and bioengineering

Wang, Xing; Hunter, Alan K; Mozier, Ned M

DOI

Application of high-performance tangential flow filtration (HPTFF) to the purification of a human pharmaceutical antibod

2008 Biotechnology and bioengineering

Lebreton, Bénédicte; Brown, Arick; van Reis, Robert

DOI

Integrated solution to purification challenges in the manufacture of a soluble recombinant protein in E. coli.

2010 Biotechnology and bioengineering

Caparon, Maire H; Rust, Kevin J; Hunter, Alan K et al.

DOI

Recovery of Chinese hamster ovary host cell proteins for proteomic analysis.

2014 Biotechnology journal

Valente, Kristin N; Schaefer, Amy K; Kempton, Hannah R et al.

DOI

The dynamics of the CHO host cell protein profile during clarification and protein A capture in a platform antibody puri

2013 Biotechnology and bioengineering

Hogwood, Catherine E M; Tait, Andrew S; Koloteva-Levine, Nadejda et al.

DOI

Anion exchange membrane adsorbers for flow‐through polishing steps: Part II. Virus, host cell protein, DNA clearance, an

2012 Biotechnology and Bioengineering

Weaver, Justin; Husson, Scott M.; Murphy, Louise et al.

DOI

Profiling of host cell proteins by two-dimensional difference gel electrophoresis (2D-DIGE): Implications for downstream

2010 Biotechnology and bioengineering

Jin, Mi; Szapiel, Nicolas; Zhang, Jennifer et al.

DOI

Evaluation of high-capacity cation exchange chromatography for direct capture of monoclonal antibodies from high-titer c

2014 Biotechnology and bioengineering

Tao, Yinying; Ibraheem, Aladein; Conley, Lynn et al.

DOI

Identification and characterization of host cell protein product‐associated impurities in monoclonal antibody bioprocess

2013 Biotechnology and Bioengineering

Levy, Nicholas E.; Valente, Kristin N.; Choe, Leila H. et al.

DOI

CHOPPI: a web tool for the analysis of immunogenicity risk from host cell proteins in CHO-based protein production.

2014 Biotechnology and bioengineering

Bailey-Kellogg, Chris; Gutiérrez, Andres H; Moise, Leonard et al.

DOI

Degradation of an Fc‐fusion recombinant protein by host cell proteases: Identification of a CHO cathepsin D protease

2009 Biotechnology and Bioengineering

Robert, Flavie; Bierau, Horst; Rossi, Mara et al.

DOI

Host cell protein dynamics in the supernatant of a mAb producing CHO cell line.

2012 Biotechnology and bioengineering

Tait, A S; Hogwood, C E M; Smales, C M et al.

DOI

Host cell protein testing by ELISAs and the use of orthogonal methods.

2014 Biotechnology and bioengineering

Zhu-Shimoni, Judith; Yu, Christopher; Nishihara, Julie et al.

DOI

Host cell protein impurities in chromatographic polishing steps for monoclonal antibody purification

2015 Biotechnology and Bioengineering

Levy, Nicholas E.; Valente, Kristin N.; Lee, Kelvin H. et al.

DOI

Host cell protein quantification by Fourier transform mid infrared spectroscopy (FT-MIR).

2013 Biotechnology and bioengineering

Capito, Florian; Skudas, Romas; Kolmar, Harald et al.

DOI

More similar than different: Host cell protein production using three null CHO cell lines.

2015 Biotechnology and bioengineering

Yuk, Inn H; Nishihara, Julie; Walker, Donald et al.

DOI

Development of a novel and efficient cell culture flocculation process using a stimulus responsive polymer to streamline

2013 Biotechnology and bioengineering

Kang, Yun Kenneth; Hamzik, James; Felo, Michael et al.

DOI

Expression of difficult-to-remove host cell protein impurities during extended Chinese hamster ovary cell culture and th

2015 Biotechnology and bioengineering

Valente, Kristin N; Lenhoff, Abraham M; Lee, Kelvin H

DOI

The future of host cell protein (HCP) identification during process development and manufacturing linked to a risk-based

2015 Biotechnology and bioengineering

Bracewell, Daniel G; Francis, Richard; Smales, C Mark

DOI

Development of robust antibody purification by optimizing protein-A chromatography in combination with precipitation met

2015 Biotechnology and bioengineering

Chollangi, Srinivas; Parker, Ray; Singh, Nripen et al.

DOI

Profiling the effects of process changes on residual host cell proteins in biotherapeutics by mass spectrometry.

2013 Biotechnology progress

Schenauer, Matthew R; Flynn, Gregory C; Goetze, Andrew M

DOI

Host cell protein adsorption characteristics during protein A chromatography.

2012 Biotechnology progress

Tarrant, Richard D R; Velez-Suberbie, M Lourdes; Tait, Andrew S et al.

DOI

Differential Response in Downstream Processing of CHO Cells Grown Under Mild Hypothermic Conditions

2013 Biotechnology progress (Print)

A. Tait; R. Tarrant; M. Velez‐Suberbie et al.

DOI

Matrix effects during monitoring of antibody and host cell proteins using attenuated total reflection spectroscopy.

2013 Biotechnology progress

Capito, Florian; Skudas, Romas; Stanislawski, Bernd et al.

DOI

A novel approach to monitor clearance of host cell proteins associated with monoclonal antibodies.

2014 Biotechnology progress

Aboulaich, Nabila; Chung, Wai Keen; Thompson, Jenny Heidbrink et al.

DOI

Select host cell proteins coelute with monoclonal antibodies in protein A chromatography.

2012 Biotechnology progress

Nogal, Bartek; Chhiba, Krishan; Emery, Jefferson C

DOI

Trace levels of the CHO host cell protease cathepsin D caused particle formation in a monoclonal antibody product.

2015 Biotechnology progress

Bee, Jared S; Tie, Liu; Johnson, Douglas et al.

DOI

Development of advanced host cell protein enrichment and detection strategies to enable process relevant spike challenge

2015 Biotechnology Progress

Soderquist, Ryan G.; Trumbo, Mihaela; Hart, Roger A. et al.

DOI

Caprylic acid-induced impurity precipitation from protein A capture column elution pool to enable a two-chromatography-s

2015 Biotechnology progress

Zheng, Ji; Wang, Lu; Twarowska, Barbara et al.

DOI

A novel aqueous two phase assisted platform for efficient removal of process related impurities associated with E. coli

2013 Journal of chromatography. A

Bhambure, Rahul; Sharma, Rohit; Gupta, Darpan et al.

DOI

Measurement and control of host cell proteins (HCPs) in CHO cell bioprocesses.

2014 Current opinion in biotechnology

Hogwood, Catherine E M; Bracewell, Daniel G; Smales, C Mark

DOI

Cation exchange versus multimodal cation exchange resins for antibody capture from CHO supernatants: identification of c

2013 Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Joucla, G; Le Sénéchal, C; Bégorre, M et al.

DOI

Cation exchange chromatography in antibody purification: pH screening for optimised binding and HCP removal.

2007 Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Stein, Andreas; Kiesewetter, André

DOI

Purification of antibodies by precipitating impurities using Polyethylene Glycol to enable a two chromatography step pro

2013 Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Giese, Glen; Myrold, Adam; Gorrell, Jeffrey et al.

DOI

Combined Protein A and size exclusion high performance liquid chromatography for the single-step measurement of mAb, agg

2014 Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Gjoka, Xhorxhi; Schofield, Mark; Cvetkovic, Aleksandar et al.

DOI

Improving impurities clearance by amino acids addition to buffer solutions for chromatographic purifications of monoclon

2015 Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Ishihara, Takashi; Hosono, Mareto

DOI

Sensitive methods for evaluation of antibodies for host cell protein analysis and screening of impurities in a vaccine p

2014 Vaccine

Lundström, Christine Sund; Mattsson, Anna; Lövgren, Karin et al.

DOI

Development of a quantitative assay for residual host cell proteins in a recombinant subunit vaccine against human respi

2001 Journal of immunological methods

Dagouassat, N; Haeuw, J F; Robillard, V et al.

DOI

Detection of contaminating enzymatic activity in plant-derived recombinant biotechnology products.

2014 Analytical chemistry

Brinson, Robert G; Giulian, Gary G; Kelman, Zvi et al.

DOI

Enhanced Detection of Low-Abundance Host Cell Protein Impurities in High-Purity Monoclonal Antibodies Down to 1 ppm Usin

2015 Analytical chemistry

Doneanu, Catalin E; Anderson, Malcolm; Williams, Brad J et al.

DOI

Quantitative host cell protein analysis using two dimensional data independent LC-MS(E).

2015 Analytical chemistry

Farrell, Amy; Mittermayr, Stefan; Morrissey, Brian et al.

DOI

Elucidation of the CHO Super-Ome (CHO-SO) by Proteoinformatics.

2015 Journal of proteome research

Kumar, Amit; Baycin-Hizal, Deniz; Wolozny, Daniel et al.

DOI

Chromatographic Analysis of Host-Cell Protein Impurities in Pharmaceuticals Derived from Recombinant DNA

1992 ACS symposium series

O'Keefe, Donald O.; Will, Mark L.

DOI

Improved Identification and Quantification of Host Cell Proteins (HCPs) in Biotherapeutics Using Liquid Chromatography-M

2015 ACS symposium series

Chen, Weibin; Doneanu, Catalin E.; Lauber, Matthew et al.

DOI

Optimized removal of soluble host cell proteins for the recovery of met-human growth hormone inclusion bodies from Esche

2007 Biotechnology progress

Venkiteshwaran, Adith; Heider, Patrick; Matosevic, Sandro et al.

DOI

Selective Leakage of Host-Cell Proteins during High-Cell-Density Cultivation of Recombinant and Non-recombinant Escheric

2004 Biotechnology Progress

Rinas, Ursula; Hoffmann, F. Michael

DOI

Demonstration of robust host cell protein clearance in biopharmaceutical downstream processes.

2008 Biotechnology progress

Shukla, Abhinav A; Jiang, Canping; Ma, Junfen et al.

DOI

Exploitation of the Adsorptive Properties of Depth Filters for Host Cell Protein Removal during Monoclonal Antibody Puri

2006 Biotechnology Progress

Yigzaw, Yinges; Piper, Robert C.; Tran, Mai H. et al.

DOI

Identifying the CHO secretome using mucin-type O-linked glycosylation and click-chemistry.

2012 Journal of proteome research

Slade, Peter G; Hajivandi, Mahbod; Bartel, Cheryl Moody et al.

DOI

Expanded bed adsorption in the purification of monoclonal antibodies: a comparison of process alternatives.

2001 Bioseparation

Blank, G S; Zapata, G; Fahrner, R et al.

DOI

Performance comparison of Protein A affinity‐chromatography sorbents for purifying recombinant monoclonal antibodies

1999 Biotechnology and Applied Biochemistry

Fahrner, Robert; Whitney, Duncan; Vanderlaan, Martin et al.

DOI

Identification and Monitoring of Host Cell Proteins by Mass Spectrometry Combined with High Performance Immunochemistry

2013 PLoS ONE

Bomans, Katrin; Lang, Antje; Roedl, Veronika et al.

DOI

INCREASED THE SPECIFICITY AND SENSITIVITY OF MONOSPECIFIC ANTIBODY AGAINST HOST CELL PROTEIN (HCP) IN QUALITY CONTROL OF

2011 Journal of paramedical sciences.

Marzieh, Hosseini Seyedeh; Bahman, Tabaraei; Shahin, Hadadian et al.

DOI

Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

2011 Journal of Visualized Experiments

Dickerson, Matthew T.; Leong, Kristen; Sheldon, Kate et al.

DOI

Developing Custom Chinese Hamster Ovary-host Cell Protein Assays using Acoustic Membrane Microparticle Technology

2011 Journal of Visualized Experiments

Dickerson, Matthew T.; Leong, Kristen; Sheldon, Kate et al.

DOI

Establishment and initial validation of an ELISA method for determination of host cell protein in PHKC vaccines

2010 Int J Biologicals

Zhao, Yuxiu; Zhang, Yuelan; Wang, Hui et al.

DOI

A microfluidic approach to high throughput quantification of host cell protein impurities for bioprocess development

2014 Pharmaceutical Bioprocessing

Heo, Jun Hyuk; Mou, Xiaodun; Wang, Fengqiang et al.

DOI

Host cell protein dynamics in recombinant CHO cells: impacts from harvest to purification and beyond.

2013 Bioengineered

Hogwood, Catherine Em; Bracewell, Daniel G; Smales, C Mark

DOI

The Comparison of Chemiluminescent- and Colorimetric-detection Based ELISA for Chinese Hamster Ovary Host Cell Proteins

2013 Journal of Bioprocessing & Biotechniques

Driscoll, Fengqiang Wang Dennis

DOI

Getting CHO host cell protein analysis up to speed

2015 Pharmaceutical Bioprocessing

Leiß, Michael; Meier, Monika; Pester, Oxana et al.

DOI

Comprehensive tracking of host cell proteins during monoclonal antibody purifications using mass spectrometry.

2014 mAbs

Zhang, Qingchun; Goetze, Andrew M; Cui, Huanchun et al.

DOI

Identification and quantification of host cell protein impurities in biotherapeutics using mass spectrometry.

2012 Analytical biochemistry

Schenauer, Matthew R; Flynn, Gregory C; Goetze, Andrew M

DOI

Analysis for residual host cell proteins and DNA in process streams of a recombinant protein product expressed in Escher

2003 Journal of pharmaceutical and biomedical analysis

Rathore, Anurag Singh; Sobacke, S E; Kocot, T J et al.

DOI

Toward the complete characterization of host cell proteins in biotherapeutics via affinity depletions, LC-MS/MS, and mul

2015 mAbs

Madsen, James A; Farutin, Victor; Carbeau, Theresa et al.

DOI

Analysis of host-cell proteins in biotherapeutic proteins by comprehensive online two-dimensional liquid chromatography/

2012 mAbs

Doneanu, Catalin E; Xenopoulos, Alex; Fadgen, Keith et al.

DOI

Reviews

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Effect of Host Cell Protein on Chinese Hamster Ovary RecombinantProtein Production and its Removal Strategies: A Mini Re

2023 Current Pharmaceutical Biotechnology

Zhao, Yaru; He, Li; Fan, Zhenlin et al.

DOI

Metadata Only

235

Investigating the combination of single‐pass tangential flow filtration and anion exchange chromatography for intensifie

2019 Biotechnology progress (Print) DOI

Enhancing Protein A performance in mAb processing: A method to reduce and rapidly evaluate host cell DNA levels during p

2019 Biotechnology Progress DOI

The effect of feed quality due to clarification strategy on the design and performance of protein A periodic counter-cur

2018 Biotechnology progress DOI

Effectiveness of host cell protein removal using depth filtration with a filter containing diatomaceous earth.

2020 Biotechnology progress DOI

Anxa2- and Ctsd-knockout CHO cell lines to diminish the risk of contamination with host cell proteins.

2019 Biotechnology progress DOI

An innovative strategy to recycle permeate in biologics continuous manufacturing process to improve material efficiency

2022 Biotechnology progress DOI

Identification of upstream culture conditions and harvest time parameters that affect host cell protein clearance.

2019 Biotechnology progress DOI

Development of an activated carbon filtration step and high throughput screening method to remove host cell proteins fro

2021 Biotechnology progress DOI

Toward optimal clearance: A universal affinity-based mass spectrometry approach for comprehensive ELISA reagent coverage

2022 Biotechnology progress DOI

Identification and characterization of a residual host cell protein hexosaminidase B associated with N-glycan degradatio

2021 Biotechnology progress DOI

Host cell proteins in monoclonal antibody processing: Control, detection, and removal.

2024 Biotechnology progress DOI

Depth filter material process interaction in the harvest of mammalian cells

2023 Biotechnology progress (Print) DOI

Proteomic analysis of host cell protein fouling during bioreactor harvesting.

2024 Biotechnology progress DOI

Proteomics‐based method to comprehensively model the removal of host cell protein impurities

2024 Biotechnology Progress DOI

Design and optimization of membrane chromatography for monoclonal antibody charge variant separation.

2022 Biotechnology progress DOI

Methods comparison of two-dimensional gel electrophoresis for host cell protein characterization.

2024 Biotechnology progress DOI

Use of LC-MS to characterize host cell protein removal during depth filtration.

2025 Biotechnology progress DOI

Establishment and evaluation of detection methods for process-specific residual host cell protein and residual host cell

2024 Cell biochemistry and function DOI

In silico mediated development of orthogonally selective mAb downstream processes for the removal of process-related imp

2025 Biotechnology progress DOI

Analytical characterization of host-cell-protein-rich aggregates in monoclonal antibody solutions.

2023 Biotechnology progress DOI

A novel approach for protein identification from complex cell proteome using modified peptide mass fingerprinting algori

2019 Electrophoresis DOI

Harnessing the power of electrophoresis and chromatography: Offline coupling of reverse phase liquid chromatography-capi

2021 Electrophoresis DOI

Risk control of host cell proteins in one therapeutic antibody produced by concentrated fed-batch (CFB) mode.

2023 Engineering in life sciences DOI

Comprehensive analysis of host cell impurities in monoclonal antibodies with improved sensitivity by capillary zone elec

2017 Electrophoresis DOI

Improving Downstream Process Related Manufacturability Based on Protein Engineering-A Feasibility Study.

2024 Engineering in life sciences DOI

Optimizing selectivity of anion hydrophobic multimodal chromatography for purification of a single-chain variable fragme

2019 Engineering in life sciences DOI

Aqueous Two-Phase-Assisted Precipitation of Proteins: A Platform for Isolation of Process-Related Impurities from Therap

2021 Methods in molecular biology (Clifton, N.J.) DOI

Development of Peptide Ligands for Targeted Capture of Host Cell Proteins from Cell Culture Production Harvests.

2021 Methods in molecular biology (Clifton, N.J.) DOI

Advanced mass spectrometry workflows for accurate quantification of trace-level host cell proteins in drug products: Ben

2023 Proteomics DOI

An innovative standard for LC-MS-based HCP profiling and accurate quantity assessment: Application to batch consistency

2021 Proteomics DOI

Removal of Residual DNA and Host Cell Proteins for the Purification of Recombinant Staphylokinase Expressed in <i>Escher

2024 Separation Science Plus DOI

Sample Preparation of Secreted Mammalian Host Cell Proteins and Their Characterization by Two-Dimensional Electrophoresi

2021 Methods in molecular biology (Clifton, N.J.) DOI

Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses.

2017 Methods in molecular biology (Clifton, N.J.) DOI

Purification of Therapeutic Antibodies by Protein A Affinity Chromatography.

2022 Methods in molecular biology (Clifton, N.J.) DOI

Analytical Tools for HCP Detection, Identification, and Quantitation.

2025 Methods in molecular biology (Clifton, N.J.) DOI

Detection of Residual Host Cell Proteins in Biotherapeutic Drugs by Concatenated 2D LC-MS/MS.

2022 Methods in molecular biology (Clifton, N.J.) DOI

Studying host cell protein based on 2D nano-LC-MS/MS: comparison between the removal and the reservation of the main ant

2025 Analytical and bioanalytical chemistry DOI

Analysis of Host Cell Proteins in Monoclonal Antibody Therapeutics Through Size Exclusion Chromatography.

2022 Pharmaceutical research DOI

Measuring Lipolytic Activity to Support Process Improvements to Manage Lipase-Mediated Polysorbate Degradation.

2020 Pharmaceutical research DOI

Ultrasensitive method for profiling host cell proteins by coupling limited digestion to ProteoMiner technology.

2022 Analytical biochemistry DOI

Development of a multiplexing method for the quantification of &quot;high-risk&quot; host cell lipases in biotherapeutic

2024 Analytica chimica acta DOI

Universal protocol and standard-spiking strategy for profiling of host cell proteins in therapeutic growth hormone.

2023 Analytical biochemistry DOI

Improved host cell protein analysis in monoclonal antibody products through ProteoMiner.

2020 Analytical biochemistry DOI

Comprehensive host cell proteins profiling in biopharmaceuticals by a sensitivity enhanced mass spectrometry strategy us

2025 Analytica chimica acta DOI

Host cell protein testing strategy for hepatitis B antigen in Hexavalent vaccine - Towards a general testing strategy fo

2018 Biologicals : journal of the International Association of Biological Standardization DOI

A highly sensitive and robust LC-MS platform for host cell protein characterization in biotherapeutics.

2023 Biologicals : journal of the International Association of Biological Standardization DOI

Characterization of host cell proteins in the downstream process of plant-Based biologics using LC-MS profiling.

2024 Biotechnology reports (Amsterdam, Netherlands) DOI

Hydrophobic interaction chromatography as polishing step enables obtaining ultra-pure recombinant antibodies.

2020 Journal of biotechnology DOI

Characterization of membrane adsorbers used for impurity removal during the continuous purification of monoclonal antibo

2020 Journal of chromatography. A DOI

A two-step heat treatment of cell disruption supernatant enables efficient removal of host cell proteins before chromato

2018 Journal of chromatography. A DOI

Residual on column host cell protein analysis during lifetime studies of protein A chromatography.

2016 Journal of chromatography. A DOI

Evaluation of chromatofocusing as a capture method for monoclonal antibody products.

2018 Journal of chromatography. A DOI

Continuous precipitation of process related impurities from clarified cell culture supernatant using a novel coiled flow

2016 Biotechnology journal DOI

Polyclonal antibodies for specific detection of tobacco host cell proteins can be efficiently generated following RuBisC

2016 Biotechnology journal DOI

Characterization of Residual Host Cell Protein Impurities in Biotherapeutics

2017 DOI

Bioinformatic analysis of Chinese hamster ovary host cell protein lipases.

2018 AIChE journal. American Institute of Chemical Engineers DOI

A combined pH and temperature precipitation step facilitates the purification of tobacco-derived recombinant proteins th

2021 Biotechnology journal DOI

Quantitative definition and monitoring of the host cell protein proteome using iTRAQ - a study of an industrial mAb prod

2016 Biotechnology journal DOI

An ultra scale-down approach identifies host cell protein differences across a panel of mAb producing CHO cell line vari

2016 Biotechnology journal DOI

Toward improved host cell protein impurity assessment

2016 Biotechnology Journal DOI

Integrated Chromatographic Platform for Simultaneous Separation of Charge Variants and Aggregates from Monoclonal Antibo

2017 Biotechnology journal DOI

Proteomics Reveals Distinctive Host Cell Protein Expression Patterns in Fed-Batch and Perfusion Cell Culture Processes.

2025 Biotechnology journal DOI

Immunoglobulin G elution in protein A chromatography employing the method of chromatofocusing for reducing the co-elutio

2017 Biotechnology and bioengineering DOI

Comparison of purification strategies for antibodies used in a broad spectrum host cell protein immunoassay.

2018 Biotechnology and bioengineering DOI

Cascading effect in bioprocessing-The impact of mild hypothermia on CHO cell behavior and host cell protein composition.

2017 Biotechnology and bioengineering DOI

Knockout of a difficult-to-remove CHO host cell protein, lipoprotein lipase, for improved polysorbate stability in monoc

2017 Biotechnology and bioengineering DOI

A novel approach to characterize host cell proteins associated with therapeutic monoclonal antibodies.

2017 Biotechnology and bioengineering DOI

Targeted capture of Chinese hamster ovary host cell proteins: Peptide ligand binding by proteomic analysis.

2020 Biotechnology and bioengineering DOI

The agony of choice: Impact of the host animal species on the enzyme-linked immunosorbent assay performance for host cel

2023 Biotechnology and bioengineering DOI

High-performance countercurrent membrane purification for host cell protein removal from monoclonal antibody products.

2023 Biotechnology and bioengineering DOI

Targeted CHO cell engineering approaches can reduce HCP-related enzymatic degradation and improve mAb product quality.

2021 Biotechnology and bioengineering DOI

Comparison of platform host cell protein ELISA to process-specific host cell protein ELISA.

2018 Biotechnology and bioengineering DOI

Best practices on critical reagent characterization, qualification, and life cycle management for HCP immunoassays.

2021 Biotechnology and bioengineering DOI

Impact of freeze-thaw processes on monoclonal antibody platform process development.

2021 Biotechnology and bioengineering DOI

Questioning coverage values determined by 2D western blots: A critical study on the characterization of anti-HCP ELISA r

2021 Biotechnology and bioengineering DOI

Improving the analytical toolbox to investigate copurifying host cell proteins presence: N-(4)-(β-acetylglucosaminyl)- l

2020 Biotechnology and bioengineering DOI

Purification processes of live virus vaccine candidates expressed in adherent Vero cell lines via multimodal chromatogra

2024 Biotechnology and bioengineering DOI

Comprehensive assessment of host cell protein expression after extended culture and bioreactor production of CHO cell li

2022 Biotechnology and bioengineering DOI

Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via &quot;flow-throu

2022 Biotechnology and bioengineering DOI

Displacement to separate host-cell proteins and aggregates in cation-exchange chromatography of monoclonal antibodies.

2021 Biotechnology and bioengineering DOI

Characterization and implications of host-cell protein aggregates in biopharmaceutical processing.

2023 Biotechnology and bioengineering DOI

&quot;High-risk&quot; host cell proteins (HCPs): A multi-company collaborative view.

2021 Biotechnology and bioengineering DOI

Comparison of host cell protein removal by depth filters with diatomaceous earth and synthetic silica filter aids using

2023 Biotechnology and bioengineering DOI

High throughput screening for rapid and reliable prediction of monovalent antibody binding behavior in flowthrough mode

2023 Biotechnology and Bioengineering DOI

Factors affecting product association as a mechanism of host-cell protein persistence in bioprocessing.

2024 Biotechnology and bioengineering DOI

Identification and characterization of CHO host-cell proteins in monoclonal antibody bioprocessing.

2024 Biotechnology and bioengineering DOI

Holistic analytical characterization and risk assessment of residual host cell protein impurities in an active pharmaceu

2022 Biotechnology and bioengineering DOI

Host cell protein networks as a novel co-elution mechanism during protein A chromatography.

2024 Biotechnology and bioengineering DOI

Assessing the Adjuvant Potential of Chinese Hamster Ovary Host Cell Proteins Using an In Vitro Dendritic Cell Assay.

2025 Biotechnology and bioengineering DOI

Quantitative Analysis Reveals Hitchhiking Drives Polysorbate Hydrolase Persistence Via Host Cell Protein-Antibody Intera

2026 Biotechnology and bioengineering DOI

Integration of Single Pass Tangential Flow Filtration and High Performance Countercurrent Membrane Purification for Inte

2025 Biotechnology and bioengineering DOI

Elucidation of Proteoforms of Chinese Hamster Ovary (CHO) Phospholipase B-Like 2 (PLBL2) Captured From a Monoclonal Anti

2026 Biotechnology and bioengineering DOI

Tailoring polishing steps for effective removal of polysorbate-degrading host cell proteins in antibody purification.

2024 Biotechnology and bioengineering DOI

Integrating Affinity Chromatography in the Platform Process for Adenovirus Purification.

2025 Biotechnology and bioengineering DOI

Buffer system improves the removal of host cell protein impurities in monoclonal antibody purification.

2024 Biotechnology and bioengineering DOI

Multi-lipase gene knockdown in Chinese hamster ovary cells using artificial microRNAs to reduce host cell protein mediat

2024 Biotechnology and bioengineering DOI

Multiple Use of Regenerated Depth Filters in Antibody Purification Processes.

2025 Biotechnology and bioengineering DOI

Characterization of the co-elution of host cell proteins with monoclonal antibodies during protein A purification.

2016 Biotechnology progress DOI

Effects of lysosomal biotherapeutic recombinant protein expression on cell stress and protease and general host cell pro

2017 Biotechnology Progress DOI

Accounting for host cell protein behavior in anion-exchange chromatography.

2016 Biotechnology progress DOI

Experience with host cell protein impurities in biopharmaceuticals.

2018 Biotechnology progress DOI

Process characterization strategy for a precipitation step for host cell protein reduction.

2020 Biotechnology progress DOI

Understanding the mechanism of copurification of &quot;difficult to remove&quot; host cell proteins in rituximab biosimi

2020 Biotechnology progress DOI

Effects of pH, conductivity, host cell protein, and DNA size distribution on DNA clearance in anion exchange chromatogra

2017 Biotechnology Progress DOI

Mass spectrometric evaluation of upstream and downstream process influences on host cell protein patterns in biopharmace

2019 Biotechnology progress DOI

Identification and tracking of problematic host cell proteins removed by a synthetic, highly functionalized nonwoven med

2019 Journal of chromatography. A DOI

Precision analysis for the determination of steric mass action parameters using eight tobacco host cell proteins.

2021 Journal of chromatography. A DOI

Formation of transient highly-charged mAb clusters strengthens interactions with host cell proteins and results in poor

2022 Journal of chromatography. A DOI

Immunoreactivity profiling of Anti-Chinese hamster ovarian host cell protein antibodies by isobaric labeled affinity pur

2022 Journal of chromatography. A DOI

Exploring features in chromatographic profiles as a tool for monitoring column performance.

2023 Journal of chromatography. A DOI

Proteomics analysis of host cell proteins after immobilized metal affinity chromatography: Influence of ligand and metal

2020 Journal of chromatography. A DOI

Behavior of host-cell-protein-rich aggregates in antibody capture and polishing chromatography.

2023 Journal of chromatography. A DOI

Biomimetic affinity chromatography for antibody purification: Host cell protein binding and impurity removal.

2023 Journal of chromatography. A DOI

Leveraging prior knowledge for process parameter classification in mAb Protein A chromatography.

2025 Journal of chromatography. A DOI

Engineering protein A ligands to mitigate antibody loss during high-pH washes in protein A chromatography.

2023 Journal of chromatography. A DOI

Purification of a non-enveloped virus using sequential aqueous two-phase extraction.

2025 Journal of Chromatography A DOI

Hydrophobicity analysis of Chinese Hamster Ovary host cell proteins using analytical hydrophobic interaction chromatogra

2025 Journal of chromatography. A DOI

Impact of ligand structure and base bead pore size on host cell protein removal during monoclonal antibody purification

2024 Journal of chromatography. A DOI

Improved clearance of host cell protein impurities at the polishing purification step using multimodal chromatography.

2024 Journal of chromatography. A DOI

Applications of proteomic methods for CHO host cell protein characterization in biopharmaceutical manufacturing.

2018 Current opinion in biotechnology DOI

Multi-component behavior of host-cell protein- and antibody-containing heteroaggregates in protein A chromatography.

2025 Journal of chromatography. A DOI

In-house CHO HCPs platform: A promising approach for HCPs ELISA monitoring.

2024 European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences DOI

Mitigating polysorbate degradation-induced visible particle formation in an antibody formulation: From root cause identi

2025 International journal of pharmaceutics DOI

Application of the Protein Maker as a platform purification system for therapeutic antibody research and development.

2016 Computational and structural biotechnology journal DOI

Assessing the polysorbate degradation fingerprints and kinetics of lipases - how the activity of polysorbate degrading h

2021 European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences DOI

Sorbitol/methanol mixed induction reduces process impurities and improves centrifugal dewatering in Pichia pastoris cult

2019 Enzyme and microbial technology DOI

SP3-based host cell protein monitoring in AAV-based gene therapy products using LC-MS/MS.

2023 European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V DOI

Integrated strategy for deep profiling of host cell proteins in downstream processing of therapeutic monoclonal antibodi

2024 European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V DOI

Cathepsin D: Removal strategy on protein A chromatography, near real time monitoring and characterisation during monoclo

2019 Journal of biotechnology DOI

SWATH-MS as a strategy for CHO host cell protein identification and quantification supporting the characterization of mA

2024 Journal of biotechnology DOI

Host cell protein identification in monoclonal antibody high molecular weight species.

2022 Journal of chromatography. B, Analytical technologies in the biomedical and life sciences DOI

Factors affecting robustness of anion exchange chromatography: Selective retention of minute virus of mice using membran

2022 Journal of chromatography. B, Analytical technologies in the biomedical and life sciences DOI

Monoclonal antibody purification using activated carbon as a replacement for Protein A affinity chromatography.

2018 Journal of chromatography. B, Analytical technologies in the biomedical and life sciences DOI

A combination of multiple LC-MS approaches for the comprehensive characterization of recombinant herpes zoster vaccine.

2025 Journal of chromatography. B, Analytical technologies in the biomedical and life sciences DOI

Application of protein A-modified capillary-channeled polymer polypropylene fibers to the quantitation of IgG in complex

2017 Journal of pharmaceutical and biomedical analysis DOI

Identification and characterization of co-purifying CHO host cell proteins in monoclonal antibody purification process.

2019 Journal of pharmaceutical and biomedical analysis DOI

Toward unbiased identification and comparative quantification of host cell protein impurities by automated iterative LC-

2021 Journal of pharmaceutical and biomedical analysis DOI

Identification of a host cell protein impurity in therapeutic protein, P1.

2017 Journal of pharmaceutical and biomedical analysis DOI

Host cell protein quantitation by LC-MS. Experimental demonstration, qualification, and comparison of methods in USP 113

2025 Journal of pharmaceutical and biomedical analysis DOI

Host cell protein quantification of an optimized purification method by mass spectrometry.

2019 Journal of pharmaceutical and biomedical analysis DOI

l-Asparaginase and HCP quantification by SWATH LC-MS/MS for new and improved purification step in Erwinia chrysanthemil-

2022 Journal of pharmaceutical and biomedical analysis DOI

Quantitation of host cell proteins in biopharmaceuticals from chinese hamster ovarian and vero cell lines using capillar

2023 Journal of pharmaceutical and biomedical analysis DOI

Comparison of analytical-flow, micro-flow and nano-flow LC-MS/MS for sub-proteome analysis.

2024 Journal of Pharmaceutical and Biomedical Analysis DOI

Host cell protein quantification workflow using optimized standards combined with data-independent acquisition mass spec

2023 Journal of pharmaceutical analysis DOI

A custom-made time-resolved fluoroimmunoassay for the quantitation of the host cell protein of Vero in rabies vaccine.

2023 Journal of virological methods DOI

Improved identification of host cell proteins in a protein biopharmaceutical by LC-MS/MS using the ProteoMiner™ Enrichme

2020 Journal of pharmaceutical and biomedical analysis DOI

Detection and quantitation of host cell proteins in monoclonal antibody drug products using automated sample preparation

2021 Journal of pharmaceutical analysis DOI

Label-free shotgun proteomics: Exploiting a reliable and sensitive method to monitor residual host-cell proteins in mono

2023 Journal of Pharmaceutical and Biomedical Analysis Open DOI

Automated optimization of resin selection, wash parameters, and elution parameters for protein A-based purifications.

2026 New biotechnology DOI

Characterization of difficult-to-remove host cell proteins in adeno-associated virus downstream processing.

2025 Molecular therapy. Methods & clinical development DOI

Effective strategies for host cell protein clearance in downstream processing of monoclonal antibodies and Fc-fusion pro

2017 Protein expression and purification DOI

Quantitative proteomic analysis of residual host cell protein retention across adeno-associated virus affinity chromatog

2024 Molecular therapy. Methods & clinical development DOI

Beyond viability: Advancing CHO cell culture process strategies to modulate host cell protein levels.

2024 New biotechnology DOI

Development of a novel engineered E. coli host cell line platform with improved column capacity performance for ion-exch

2018 Protein expression and purification DOI

Evaluation of spectral libraries and sample preparation for DIA-LC-MS analysis of host cell proteins: A case study of a

2018 Protein expression and purification DOI

Sodium caprylate induced precipitation post Protein A chromatography as an effective means for host cell protein clearan

2019 Protein expression and purification DOI

pH conditioning is a crucial step in primary recovery - A case study for a recombinant Fab from E. coli.

2020 Protein expression and purification DOI

A two-step process for capture and purification of human basic fibroblast growth factor from E. coli homogenate: Yield v

2019 Protein expression and purification DOI

Promiscuity of host cell proteins in the purification of histidine tagged recombinant xylanase A by IMAC procedures: A c

2019 Protein expression and purification DOI

Evaluating the efficacy of immobilized metal affinity chromatography (IMAC) for host cell protein (HCP) removal from ant

2022 Protein expression and purification DOI

Removal of polysorbates degradation-related impurities by depth filter screening: A case study.

2026 Protein expression and purification DOI

The advantage of high pH eluting Protein A resins over their regular counterparts in aggregate and host cell protein cle

2026 Protein expression and purification DOI

The adequate amount of sodium chloride in Protein A wash buffer for effective host cell protein clearance.

2019 Protein expression and purification DOI

Two major mechanisms contributing to copurification of CHO host cell proteins and strategies to minimize their negative

2022 Protein expression and purification DOI

Deciphering the inconsistent performance of Protein A membrane on host cell protein clearance.

2026 Protein expression and purification DOI

Integrated native digestion and molecular weight cutoff enrichment with DIA-MS: a sensitive and selective approach for q

2026 Talanta DOI

Degradation of Polysorbate 20 by Sialate O-Acetylesterase in Monoclonal Antibody Formulations.

2021 Journal of pharmaceutical sciences DOI

Identification and Quantification of a Problematic Host Cell Protein to Support Therapeutic Protein Development.

2023 Journal of pharmaceutical sciences DOI

Polysorbate Degradation and Particle Formation in a High Concentration mAb: Formulation Strategies to Minimize Effect of

2021 Journal of pharmaceutical sciences DOI

Identification and Characterization of Polysorbate-Degrading Enzymes in a Monoclonal Antibody Formulation.

2021 Journal of pharmaceutical sciences DOI

Improved identification of host cell proteins in monoclonal antibodies by combining filter-aided sample preparation and

2025 Journal of pharmaceutical sciences DOI

A workflow for accurate and consistent quantitation of host cell proteins by SWATH LC-MS/MS analysis to support process

2025 Journal of pharmaceutical sciences DOI

Characterization of Recombinantly-Expressed Hydrolytic Enzymes from Chinese Hamster Ovary Cells: Identification of Host

2023 Journal of pharmaceutical sciences DOI

A Highly Sensitive LC-MS/MS Method for Targeted Quantitation of Lipase Host Cell Proteins in Biotherapeutics.

2021 Journal of pharmaceutical sciences DOI

Host cell protein detection via surfactant-assisted acid precipitation.

2025 Journal of pharmaceutical sciences DOI

Investigating pH Effects on Enzymes Catalyzing Polysorbate Degradation by Activity-Based Protein Profiling.

2024 Journal of pharmaceutical sciences DOI

Identification of Acyl-Protein Thioesterase-1 as a Polysorbate-Degrading Host Cell Protein in a Monoclonal Antibody Form

2024 Journal of pharmaceutical sciences DOI

Elucidating uptake and metabolic fate of dipeptides in CHO cell cultures using 13C labeling experiments and kinetic mode

2024 Metabolic Engineering DOI

Fast, Robust, and Sensitive Identification of Residual Host Cell Proteins in Recombinant Monoclonal Antibodies Using Sod

2020 Analytical chemistry DOI

Ultrasensitive Quantification Method for Understanding Biologically Relevant Concentrations of Host Cell Proteins in The

2023 Analytical chemistry DOI

A Novel Sample Preparation for Shotgun Proteomics Characterization of HCPs in Antibodies.

2017 Analytical chemistry DOI

Enhancing Host-Cell Protein Detection in Protein Therapeutics Using HILIC Enrichment and Proteomic Analysis.

2020 Analytical chemistry DOI

Host Cell Protein Profiling by Targeted and Untargeted Analysis of Data Independent Acquisition Mass Spectrometry Data w

2017 Analytical chemistry DOI

Simple and Sensitive Method for Deep Profiling of Host Cell Proteins in Therapeutic Antibodies by Combining Ultra-Low Tr

2021 Analytical chemistry DOI

Combination of FAIMS, Protein A Depletion, and Native Digest Conditions Enables Deep Proteomic Profiling of Host Cell Pr

2020 Analytical chemistry DOI

Standard-Free Absolute Quantitation of Antibody Deamidation Degradation and Host Cell Proteins by Coulometric Mass Spect

2022 Analytical Chemistry DOI

Versatile LC-MS-Based Workflow with Robust 0.1 ppm Sensitivity for Identifying Residual HCPs in Biotherapeutic Products.

2022 Analytical chemistry DOI

Native Digestion and Shotgun Proteomics for Host Cell Protein Profiling of Adeno-Associated Viruses.

2024 Analytical chemistry DOI

A 2D LC-MS/MS Strategy for Reliable Detection of 10-ppm Level Residual Host Cell Proteins in Therapeutic Antibodies.

2018 Analytical chemistry DOI

diaPASEF Enables High-Throughput Proteomic Analysis of Host Cell Proteins for Biopharmaceutical Process Development.

2024 Analytical chemistry DOI

Host Cell Protein Profiling in Biopharmaceutical Harvests

2018 Analytical Chemistry DOI

Targeted Host Cell Protein Quantification by LC-MRM Enables Biologics Processing and Product Characterization.

2020 Analytical chemistry DOI

Strategy for Generating Sequence-Defined Aptamer Reagent Sets for Detecting Protein Contaminants in Biotherapeutics.

2018 Analytical chemistry DOI

Dual Data-Independent Acquisition Approach Combining Global HCP Profiling and Absolute Quantification of Key Impurities

2018 Analytical chemistry DOI

Sensitive, Rapid, Robust, and Reproducible Workflow for Host Cell Protein Profiling in Biopharmaceutical Process Develop

2020 Journal of proteome research DOI

Evaluation of Peptide Fractionation and Native Digestion as Two Novel Sample Preparation Workflows to Improve HCP Charac

2019 Analytical chemistry DOI

Optimized Sample Preparation and Data Processing of Data-Independent Acquisition Methods for the Robust Quantification o

2021 Journal of proteome research DOI

Proteomics Strategies for Mass Spectrometry Analysis of Host Cell Proteins in Monoclonal Antibody Bioprocessing.

2026 Journal of proteome research DOI

Development of an Optimized LC-MS Workflow for Host Cell Protein Characterization to Support Upstream Process Developmen

2025 Journal of proteome research DOI

Carrier-Guided Proteome Analysis in a High Protein Background: An Improved Approach to Host Cell Protein Identification.

2024 Journal of proteome research DOI

A Novel Strategy to Rapidly Profile and Quantify High-Risk Host Cell Proteins Using Integrated DDA-PRM-dMRM Mode.

2025 Journal of proteome research DOI

High-Risk Host Cell Protein Profiling with Sub-ppm Sensitivity by iRT-Assisted Targeted Mass Spectrometry.

2026 Journal of proteome research DOI

The Dual-Integrated Multi-Attribute Method: Simultaneous Evaluation of Post-Translational Modifications and Host Cell Pr

2026 ACS omega DOI

Systematic Optimization of Activity-Based Protein Profiling for Identification of Polysorbate-Degradative Enzymes in Bio

2024 Journal of the American Society for Mass Spectrometry DOI

Tracking the Behavior of Monoclonal Antibody Product Quality Attributes Using a Multi-Attribute Method Workflow.

2021 Journal of the American Society for Mass Spectrometry DOI

A modular and adaptive mass spectrometry-based platform for support of bioprocess development toward optimal host cell p

2017 mAbs DOI

Host cell protein profiling of commercial therapeutic protein drugs as a benchmark for monoclonal antibody-based therape

2021 mAbs DOI

Technical advancement and practical considerations of LC-MS/MS-based methods for host cell protein identification and qu

2023 mAbs DOI

ELISA reagent coverage evaluation by affinity purification tandem mass spectrometry.

2017 mAbs DOI

Insights into ultra-low affinity lipase-antibody noncovalent complex binding mechanisms.

2022 mAbs DOI

Host Cell Proteins Can Be Eliminated More Strategically

2022 Genetic Engineering &amp; Biotechnology News DOI

Illuminating a biologics development challenge: systematic characterization of CHO cell-derived hydrolases identified in

2024 mAbs DOI

Hamster neogenin, a host-cell protein contained in a respiratory syncytial virus candidate vaccine, induces antibody res

2020 Human Vaccines & Immunotherapeutics DOI

Mild hypothermic culture conditions affect residual host cell protein composition post-Protein A chromatography.

2018 mAbs DOI

Host cell protein detection gap risk mitigation: quantitative IAC-MS for ELISA antibody reagent coverage determination.

2021 mAbs DOI

Ceramic hydroxyapatite chromatography plays a critical role in bispecific antibody purification process for impurity rem

2023 Antibody therapeutics DOI

Evaluating Immunogenicity Risk Due to Host Cell Protein Impurities in Antibody-Based Biotherapeutics.

2016 The AAPS journal DOI

Improving Chinese hamster ovary host cell protein ELISA using Ella®: an automated microfluidic platform.

2020 BioTechniques DOI

Impact of next-generation high productivity perfusion cell culture process on host cell protein profile and a comparison

2020 DOI

Development of a One-step Magnetic Separation-based Fluorescent Sandwich Assay for Residual Protein A in Antibody Drugs

2025 Current Analytical Chemistry DOI

Cascading effects in bioprocessing: the impact of cell culture environment on CHO cell behaviour and host cell protein s

2016 Spiral (Imperial College London) DOI

Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts.

2016 Journal of visualized experiments : JoVE DOI

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion

2018 Journal of visualized experiments : JoVE DOI

Host Cell Protein Analysis using Enrichment Beads Coupled with Limited Digestion.

2024 Journal of visualized experiments : JoVE DOI

Comparison of Tobacco Host Cell Protein Removal Methods by Blanching Intact Plants or by Heat Treatment of Extracts.

2016 Journal of visualized experiments : JoVE DOI

Process- and product-related impurities in the ChAdOx1 nCov-19 vaccine.

2022 eLife DOI

Experimental characterization and prediction of Escherichia coli host cell proteome retention during preparative chromat

2024 Biotechnology and bioengineering DOI

A novel approach to evaluate ELISA antibody coverage of host cell proteins-combining ELISA-based immunocapture and mass

2020 Biotechnology progress DOI

Identification and classification of host cell proteins during biopharmaceutical process development.

2022 Biotechnology progress DOI

Development of continuous processing platform utilizing aqueous two-phase extraction for purification of monoclonal anti

2024 Journal of chromatography. A DOI

Efficient host cell protein clearance: A study of membrane adsorbers and resins in biopharmaceutical processes.

2025 Journal of chromatography. B, Analytical technologies in the biomedical and life sciences DOI

A new deep field scan liquid chromatography-mass spectrometry method to identify host cell proteins in therapeutic prote

2026 Journal of pharmaceutical and biomedical analysis DOI

Improved Host Cell Protein Analysis in Monoclonal Antibody Products through Molecular Weight Cutoff Enrichment.

2020 Analytical chemistry DOI

Advanced assessment of the physicochemical characteristics of Remicade® and Inflectra® by sensitive LC/MS techniques.

2016 mAbs DOI

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion

2018 Journal of visualized experiments : JoVE DOI

Needs Review

14
No corresponding author in OpenAlex
2024 Frontiers in bioengineering and biotechnology DOI
patrick.garidel@boehringer-ingelheim.com
No corresponding author in OpenAlex
2023 Analytical and Bioanalytical Chemistry DOI
gavin.oconnor@ptb.de
No corresponding author in OpenAlex
2020 Analytical and bioanalytical chemistry DOI
c.huber@sbg.ac.at
No corresponding author in OpenAlex
2022 Analytical and bioanalytical chemistry DOI
mortz@alphalyse.com
No corresponding author in OpenAlex
2018 Virology journal DOI
dora.sviben@gmail.com
No corresponding author in OpenAlex
2023 The AAPS journal DOI
AnnieD@EpiVax.com
No corresponding author in OpenAlex
2018 PloS one DOI
dyang@whu.edu.cn
No corresponding author in OpenAlex
2023 PloS one DOI
zhang_wei@bti.a-star.edu.sg
No corresponding author in OpenAlex
2023 PloS one DOI
zenanmail@icloud.com
No corresponding author in OpenAlex
2025 Frontiers in bioengineering and biotechnology DOI
KHL@udel.edu
No corresponding author in OpenAlex
2021 Haematologica DOI
andreas.greinacher@med.uni-greifswald.de, voelker@uni-greifswald.de
No corresponding author in OpenAlex
2021 Biotech (Basel (Switzerland)) DOI
arikm@iibr.gov.il, AvitalJayson@iibr.gov.il, zivo@iibr.gov.il
No corresponding author in OpenAlex
2019 International journal of molecular sciences DOI
rabradl3@ncsu.edu, abdifazi@ncsu.edu, ruben@ncsu.edu
No corresponding author in OpenAlex
2024 Mass spectrometry (Tokyo, Japan) DOI
yishiham@pharm.kyoto-u.ac.jp

Author Found

22
Gyun Min; Lewis, Nathan E

nlewisres@ucsd.edu

2020 Nature communications
DOI
Gyun Min

gyunminlee@kaist.ac.kr

2017 Scientific reports
DOI
Li, Xuanwen; An

xuanwen.li@merck.com

2021 Biotechnology progress
DOI
Khalil, Somar; Plisnier

Somar.x.khalil@gsk.com

2025
DOI
Yuansheng; Zhang, Wei

zhang_wei@bti.a-star.edu.sg

2022 Bioresources and bioprocessing
DOI
Alhuthali, Sakhr; Kontoravdi

s.alhuthali15@imperial.ac.uk

2022 PloS one
DOI
Marzluf, Jannis Peter; Klein

jannis.marzluf@sartorius.com

2026 Frontiers in bioengineering and biotechnology
DOI
Kornecki, Martin; Mestmäcker

kornecki@itv.tu-clausthal.de

2017 Antibodies (Basel, Switzerland)
DOI
Volkan; Buyel, Johannes F

johannes.buyel@rwth-aachen.de

2018 Frontiers in bioengineering and biotechnology
DOI
Xuemei; Pan, Hongjuan; Chen

panhongjuan@sinopharm.com

2025 Frontiers in microbiology
DOI
Rainer; Buyel, Johannes F

johannes.buyel@ime.fraunhofer.de

2016 Frontiers in plant science
DOI
Amith D; Hashimoto, Yasuhiro; Menegatti

y-hashimoto@fuji-silysia.co.jp

2019 International journal of molecular sciences
DOI
Peng George; Xiao, Weidong

wxiao@temple.edu

2018 Viruses
DOI
Lemma, Solomon Mengistu; Boi

smlemma@ncsu.edu

2021 Membranes
DOI
Alice; Blackburn, R Kevin; Goshe

Kevin_Blackburn@waters.com

2019 International journal of molecular sciences
DOI
A I De; Du, Min

min.du@thermofisher.com

2024 International journal of molecular sciences
DOI
Liu, Sixu; Li

liusixu2020@163.com

2024 Viruses
DOI
Flecheux, Julie; Bardet

julie.flecheux@univ-lyon1.fr

2026 Proteomes
DOI
Natalia; Tubbs, Christopher M; Loughney

christopher.tubbs@merck.com

2024 Vaccines
DOI
Elianet; Bernier, Alice; Cervera

bernier.alice@videotron.ca

2021 Vaccines
DOI
Mo; Li, Min

mirandali418@sina.com

2024 Vaccines
DOI
Danhua; Li, Yuhua

liyuhua@nifdc.org.cn

2025 Vaccines
DOI