Monoclonal Antibody Production Optimization In Cho

Glycoprotein (GP) Ib-IX-transfected Cells Roll on a von Willebrand Factor Matrix under Flow

Cranmer, Susan L.; Ulsemer, Philippe; Cooke, Brian M. et al.

Aggresomes: A Cellular Response to Misfolded Proteins

Johnston, Jennifer; Ward, Cristina L.; Kopito, Ron R.

Recombinant soluble human Fc gamma RII: production, characterization, and inhibition of the Arthus reaction.

Ierino, Frank; Powell, Maree S.; McKenzie, I F et al.

Production of non-fucosylated antibodies by co-expression of heterologous GDP-6-deoxy-D-lyxo-4-hexulose reductase

Horsten, Hans Henning von; Ogorek, Christiane; Blanchard, Véronique et al.

A general method for the selection of high-level scFv and IgG antibody expression by stably transfected mammalian cells

Zuberbühler, Klaus; Palumbo, Alessandro; Bacci, Cristina et al.

Heterologous protein production using euchromatin-containing expression vectors in mammalian cells

Zboray, Katalin; Sommeregger, Wolfgang; Bogner, Edith et al.

Fc glycans of therapeutic antibodies as critical quality attributes

Reusch, Dietmar; Tejada, Max L.

Expression of Antibody Fragments with a Controlled<i>N</i>-Glycosylation Pattern and Induction of Endoplasmic Reticulum-

Loos, Andreas; Droogenbroeck, Bart Van; Hillmer, Stefan et al.

<i>pavarotti</i> encodes a kinesin-like protein required to organize the central spindle and contractile ring for cytoki

Adams, Richard R.; Tavares, Álvaro A.; Salzberg, Adi et al.

Structural and biological properties of human recombinant myeloperoxidase produced by Chinese hamster ovary cell lines

Moguilevsky, Nicole; Garcia-Quintana, Lida; Jacquet, Alain et al.

From gene to harvest: insights into upstream process development for the GMP production of a monoclonal antibody in tran

M. Sack; T. Rademacher; H. Spiegel et al.

Aggregates, Crystals, Gels, and Amyloids: Intracellular and Extracellular Phenotypes at the Crossroads of Immunoglobulin

Hasegawa, Haruki

Biological Insights into Therapeutic Protein Modifications throughout Trafficking and Their Biopharmaceutical Applicatio

Zhong, Xiaotian; Wright, Jill F.

Early Implementation of QbD in Biopharmaceutical Development: A Practical Example

Zurdo, Jesús; Arnell, Andreas; Obrezanova, Olga et al.

Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection

Schiedner, Gudrun; Hertel, Sabine; Bialek, Corinna et al.

Establishment and mitotic stability of an extra-chromosomal mammalian replicon

Stehle, Isa M.; Postberg, Jan; Rupprecht, Sina et al.

Molecular cloning, characterization, genomic organization and promoter analysis of the α1,6-fucosyltransferase gene (fut

Teylaert, Béatrice; Meurice, Edwige; Bobowski, Marie et al.

Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins

Caron, Antoine W.; Nicolas, Claire; Gaillet, Bruno et al.

Generation and characterization of a new mammalian cell line continuously expressing virus-like particles of Japanese en

Hua, Rong-Hong; Li, Yenan; Chen, Zhenshi et al.

Double knockdown of α1,6-fucosyltransferase (FUT8) and GDP-mannose 4,6-dehydratase (GMD) in antibody-producing cells: a

Imai-Nishiya, Harue; Mori, Katsuhiro; Inoue, Miho et al.

Generation of diploid Pichia pastoris strains by mating and their application for recombinant protein production

Chen, Ming‐Tang; Lin, Song; Shandil, Ishaan et al.

Applications of cell sorting in biotechnology

Mattanovich, Diethard; Borth, Nicole

Stable 293 T and CHO cell lines expressing cleaved, stable HIV-1 envelope glycoprotein trimers for structural and vaccin

Chung, Nancy PY; Matthews, Katie; Kim, Helen J. et al.

Recombinant protein expression by targeting pre-selected chromosomal loci

Nehlsen, Kristina; Schucht, Roland; Gama-Norton, Leonor et al.

Production and characterization of specific monoclonal antibodies binding the Plasmodium falciparum diagnostic biomarker

Leow, Chiuan Yee; Jones, Martina L.; Cheng, Qin et al.

Novel cell engineering of the Unfolded Protein Response to achieve efficient therapeutic protein production cell line

Michael Song; J. Hou; L. Nielsen et al.

High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells

Jäger, Volker; Büssow, Konrad; Wagner, Andreas et al.

Structural and molecular basis for hyperspecificity of RNA aptamer to human immunoglobulin G

Miyakawa, Shin; Nomura, Yusuke; Sakamoto, Taiichi et al.

Intraclonal Protein Expression Heterogeneity in Recombinant CHO Cells

Pilbrough, Warren; Munro, Trent P.; Gray, Peter P.

SweetBac: A New Approach for the Production of Mammalianised Glycoproteins in Insect Cells

Palmberger, Dieter; Wilson, Iain B. H.; Berger, Imre et al.

Efficient Recombinant Production in Mammalian Cells Using a Novel IR/MAR Gene Amplification Method

Araki, Yoshio; Hamafuji, Tetsuro; Noguchi, Chiemi et al.

Systematic Single-Cell Analysis of Pichia pastoris Reveals Secretory Capacity Limits Productivity

Love, Kerry R.; Politano, Timothy J.; Panagiotou, Vasiliki et al.

Rapid Transient Production in Plants by Replicating and Non-Replicating Vectors Yields High Quality Functional Anti-HIV

Sainsbury, Frank; Sack, M.; Stadlmann, Johannes et al.

An Internal Ribosome Entry Site (IRES) Mutant Library for Tuning Expression Level of Multiple Genes in Mammalian Cells

Koh, Esther Y. C.; Ho, Steven C. L.; Mariati et al.

A Multi-Omics Analysis of Recombinant Protein Production in Hek293 Cells

Dietmair, Stefanie; Hodson, Mark P.; Quek, Lake‐Ee et al.

Fusion of the Dhfr/Mtx and IR/MAR Gene Amplification Methods Produces a Rapid and Efficient Method for Stable Recombinan

Noguchi, Chiemi; Araki, Yoshio; Miki, Daisuke et al.

Multiparameter RNA and Codon Optimization: A Standardized Tool to Assess and Enhance Autologous Mammalian Gene Expressio

Fath, Stephan; Bauer, Asli; Liss, Michael A. et al.

Biochemical Characterization of Human Anti-Hepatitis B Monoclonal Antibody Produced in the Microalgae Phaeodactylum tric

Vanier, Gaëtan; Hempel, Franziska; Chan, Philippe et al.

Generation of Biologically Active Multi-Sialylated Recombinant Human EPOFc in Plants

Castilho, Alexandra; Neumann, Laura; Gattinger, Pia et al.

Comparison of Internal Ribosome Entry Site (IRES) and Furin-2A (F2A) for Monoclonal Antibody Expression Level and Qualit

Ho, Steven C. L.; Bardor, Muriel; Li, Bin et al.

Transgenic Production of an Anti HIV Antibody in the Barley Endosperm

Hensel, Göetz; Floß, Doreen M.; Arcalís, Elsa et al.

Optimization of Heavy Chain and Light Chain Signal Peptides for High Level Expression of Therapeutic Antibodies in CHO C

Haryadi, Ryan; Ho, Steven C. L.; Kok, Yee Jiun et al.

Analyzing Clonal Variation of Monoclonal Antibody-Producing CHO Cell Lines Using an In Silico Metabolomic Platform

Ghorbaniaghdam, Atefeh; Chen, Jingkui; Henry, Olivier et al.

REAL-Select: Full-Length Antibody Display and Library Screening by Surface Capture on Yeast Cells

Rhiel, Laura; Krah, Simon; Günther, Ralf et al.

Chromatin Function Modifying Elements in an Industrial Antibody Production Platform - Comparison of UCOE, MAR, STAR and

Saunders, Fay Louise; Sweeney, Berni; Antoniou, Michael et al.

The Inhibition Site on Myelin-Associated Glycoprotein Is within Ig-Domain 5 and Is Distinct from the Sialic Acid Binding

Cao, Zixuan; Qiu, Jin; Domeniconi, Marco et al.

Development of transfection and high-producer screening protocols for the CHOK1SV cell system

M. Celina de la Cruz Edmonds; M. Tellers; Christine Chan et al.

Monoclonal antibodies for the prevention of rabies: theory and clinical practice

Nagarajan, Thirumeni; Marissen, Wilfred E.; Rupprecht, Charles E.

High-yield production of recombinant antibody fragments in HEK-293 cells using sodium butyrate.

J. Grünberg; K. Knogler; R. Waibel et al.

Advances in improving mammalian cells metabolism for recombinant protein production

Altamirano, Claudia; Berríos, Julio; Vergara, Mauricio et al.

Production systems for recombinant antibodies

Schirrmann, Thomas

Function and 3D Structure of the N-Glycans on Glycoproteins

Nagae, Masamichi; Yamaguchi, Yoshiki

Trends in Upstream and Downstream Process Development for Antibody Manufacturing

Gronemeyer, Petra; Ditz, Reinhard; Strube, Jochen

Advances in Mammalian Cell Line Development Technologies for Recombinant Protein Production

Lai, Tingfeng; Yang, Yuansheng; Ng, Say Kong

Identification of Cystatin SN as a novel tumor marker for colorectal cancer.

K. Yoneda; Hiroshi Iida; Hiroki Endo et al.

<i>Pichia pastoris</i> secretes recombinant proteins less efficiently than Chinese hamster ovary cells but allows higher

Maccani, Andreas; Landes, Nils; Stadlmayr, Gerhard et al.

Defining process design space for monoclonal antibody cell culture.

Abu-Absi, Susan Fugett; Yang, LiYing; Thompson, Patrick et al.

Use of the chicken lysozyme 5′ matrix attachment region to generate high producer CHO cell lines

Girod, Pierre‐Alain; Zahn‐Zabal, Monique; Mermod, Nicolas

A study of monoclonal antibody‐producing CHO cell lines: What makes a stable high producer?

Chusainow, Janet; Yang, Yuansheng; Yeo, Jessna H. M. et al.

Engineering mammalian cell factories for improved recombinant monoclonal antibody production: lessons from nature?

Dinnis, Diane M.; James, David C.

Rapid whole monoclonal antibody analysis by mass spectrometry: An ultra scale-down study of the effect of harvesting by

Reid, C Q; Tait, A; Baldascini, H et al.

Assurance of monoclonality in one round of cloning through cell sorting for single cell deposition coupled with high res

Evans, Krista; Albanetti, Thomas; Venkat, Raghavan et al.

Insertion of core CpG island element into human CMV promoter for enhancing recombinant protein expression stability in C

Mariati; Yeo, Jessna H. M.; Koh, Esther Y. C. et al.

Production and scale‐up of a monoclonal antibody against 17‐hydroxyprogesterone

G. K. Chua; B. Abdul-Rahman; Y. Chisti

Quick generation of R aman spectroscopy based in‐process glucose control to influence biopharmaceutical protein product

Berry, Brandon; Dobrowsky, Terrence M.; Timson, Rebecca C. et al.

N‐linked glycosylation is an important parameter for optimal selection of cell lines producing biopharmaceutical human I

Berkel, Patrick H.C. van; Gerritsen, Jolanda; Perdok, Gerrard J. et al.

CHO cells adapted to hypothermic growth produce high yields of recombinant β‐interferon

Sunley, Kevin; Tharmalingam, Tharmala; Butler, Michael

Monoclonal antibodies to human interferon‐gamma: production, affinity purification and radioimmunoassay.

D. Novick; Zelig Eshhar; D. Fischer et al.

Application of a quality by design approach to the cell culture process of monoclonal antibody production, resulting in

Nagashima, Hiroaki; Watari, Akiko; Shinoda, Yasuharu et al.

Over‐expression of secreted proteins from mammalian cell lines

Dalton, Annamarie C.; Barton, William A.

Screening and subcloning of high producer transfectomas using semisolid media and automated colony picker.

S. Dharshanan; C. S. Hung

Rapid construction of transgene-amplified CHO cell lines by cell cycle checkpoint engineering

K. Lee; Masayoshi Onitsuka; K. Honda et al.

Methods for production of monoclonal antibodies with specificity for human lung cancer cells

J. Minna; F. Cuttitta; S. Rosen et al.

Current state and recent advances in biopharmaceutical production in <i>Escherichia coli</i>, yeasts and mammalian cells

Berlec, Aleš; Štrukelj, Borut

Using cell engineering and omic tools for the improvement of cell culture processes

Kuystermans, Darrin; Krampe, Britta; Swiderek, Halina et al.

Repeated integration of antibody genes into a pre-selected chromosomal locus of CHO cells using an accumulative site-spe

Kawabe, Yoshinori; Makitsubo, Hirokatsu; Kameyama, Yujiro et al.

Characterization of a monoclonal antibody cell culture production process using a quality by design approach.

Horvath, Brian; Mun, Melissa; Laird, Michael W

Single-Batch Production of Recombinant Human Polyclonal Antibodies

Nielsen, Lars S.; Baer, Alexandra; Müller, Christian et al.

Physicochemical and Functional Comparability Between the Proposed Biosimilar Rituximab GP2013 and Originator Rituximab

Visser, Jan Marinus; Feuerstein, Isabel; Stangler, Thomas et al.

Stability of single copy transgene expression in CHOK1 cells is affected by histone modifications but not by DNA methyla

Spencer, Shawal; Gugliotta, Agustina; Koenitzer, Jennifer et al.

Regulated overexpression of the survival factor bcl-2 in CHO cells increases viable cell density in batch culture and de

Fussenegger, Martin; Fassnacht, D.; Schwartz, Regine et al.

The gel microdrop secretion assay: Identification of a low productivity subpopulation arising during the production of h

Hammill, Linda; Welles, J L; Carson, Gerald

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line

Xu, Xun; Nagarajan, Harish; Lewis, Nathan E. et al.

A series of Fas receptor agonist antibodies that demonstrate an inverse correlation between affinity and potency

Chodorge, Matthieu; Züger, Sara; Stirnimann, Christian U. et al.

Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG

Fischer, Nicolas; Elson, Greg; Magistrelli, Giovanni et al.

Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells

H. Hansen; Claes Nymand Nilsson; A. Lund et al.

Engineering and characterisation of chimeric monoclonal antibody 806 (ch806) for targeted immunotherapy of tumours expre

Panousis, Con; Rayzman, Veronika; Johns, Terrance G. et al.

CMV promoter mutants with a reduced propensity to productivity loss in CHO cells

Moritz, Benjamin; Becker, Peter B.; Göpfert, Ulrich

Simple <i>piggyBac</i> transposon-based mammalian cell expression system for inducible protein production

Li, Zhijie; Michael, Iacovos P.; Zhou, Dongxia et al.

Recombinant Dimeric IgA Antibodies against the Epidermal Growth Factor Receptor Mediate Effective Tumor Cell Killing

Lohse, Stefan; Derer, Stefanie; Beyer, Thomas et al.

Cell culture processes for monoclonal antibody production.

Li, Feng; Vijayasankaran, Natarajan; Shen, Amy Yijuan et al.

Control of Key Parameters in Developing Mammalian Production Clones

Kunert, Renate; Wolbank, Susanne; Chang, Martina et al.

[Production of anti-recombinant human arrest defective 1 protein (hARD1) monoclonal antibodies for assaying human breast

Min Yu; Zehua Wang; Junli Gong et al.

On enzymatic remodeling of IgG glycosylation; unique tools with broad applications

Sjögren, Jonathan; Lood, Rolf; Nägeli, Andreas

Protein N-glycosylation in eukaryotic microalgae and its impact on the production of nuclear expressed biopharmaceutical

Mathieu‐Rivet, Elodie; Kiefer‐Meyer, Marie‐Christine; Vanier, Gaëtan et al.

Sophisticated Cloning, Fermentation, and Purification Technologies for an Enhanced Therapeutic Protein Production: A Rev

Gupta, Sanjeev Kumar; Shukla, Pratyoosh

Putting the Spotlight Back on Plant Suspension Cultures

Santos, Rita B.; Abranches, Rita; Fischer, Rainer et al.

Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring

Jorgolli, Marsela; Nevill, Tanner; Winters, Aaron et al.

Transposon vector-mediated stable gene transfer for the accelerated establishment of recombinant mammalian cell pools al

Natalie Tschorn; K. Berg; J. Stitz

Albumin and mammalian cell culture: implications for biotechnology applications

Francis, Geoffrey L.

Developing the Totality of Evidence for Biosimilars: Regulatory Considerations and Building Confidence for the Healthcar

Márkus, Richárd; Liu, Jennifer; Ramchandani, Monica et al.

Mammalian cell culture for production of recombinant proteins: A review of the critical steps in their biomanufacturing

O’Flaherty, Róisín; Bergin, Adam; Flampouri, Evangelia et al.

Genetic glycoengineering in mammalian cells

Narimatsu, Yoshiki; Büll, Christian; Chen, Yen‐Hsi et al.

Bioprocessing of mAb (Monoclonal Antibodies) using Chinese Hamster Ovary (CHO) Cells: A Review

Richelle Bertly Josefano; Aivyanca A; Charlivo Mikaichi Dungus et al.

Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Tripathi, Nagesh K.; Shrivastava, Ambuj

Expression of Recombinant Antibodies

Frenzel, André; Hust, Michael; Schirrmann, Thomas

Improving Immunotherapy Through Glycodesign

Buettner, Matthew J.; Shah, Sagar; Saeui, Christopher T. et al.