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Inducible HEK293 AAV packaging cell lines expressing Rep proteins.
Production of rVSV-ZEBOV in serum-free suspension culture of HEK 293SF cells
Improving viral-based gene delivery to mammalian cells through simple co-incubation with transportan peptide in vitro.
Comparison of highly pure rAAV9 vector stocks produced in suspension by PEI transfection or HSV infection reveals striki
Establishment of a novel human amniotic epithelial-derived cell line, HAT, for high-yield AAV vector production.
Efficient production of recombinant adeno-associated viral vector, serotype DJ/8, carrying the GFP gene.
Suppression of toxic transgene expression by optimized artificial miRNAs increases AAV vector yields in HEK-293 cells.
Producing high-quantity and high-quality recombinant adeno-associated virus by low-cis triple transfection.
Enhanced AAV production via rational design of a novel pHelper vector integrated with HSV-1 helper genes.
Multi-omics driven genome-scale metabolic modeling improves viral vector yield in HEK293.
Interplay between Triadin and Calsequestrin in the Pathogenesis of CPVT in the Mouse.
Characterization of a spontaneously generated murine retinal pigmented epithelium cell line; a model for in vitro experi
Systematic comparison of rAAV vectors manufactured using large-scale suspension cultures of Sf9 and HEK293 cells.
Ultracentrifugation-free chromatography-mediated large-scale purification of recombinant adeno-associated virus serotype
Production of Recombinant Adeno-associated Virus Vectors Using Suspension HEK293 Cells and Continuous Harvest of Vector
Sodium Chloride Enhances Recombinant Adeno-Associated Virus Production in a Serum-Free Suspension Manufacturing Platform
Continuous Collection of Adeno-Associated Virus from Producer Cell Medium Significantly Increases Total Viral Yield.
Development and Optimization of AAV hFIX Particles by Transient Transfection in an iCELLis(®) Fixed-Bed Bioreactor.
Standardized, Scalable, and Timely Flexible Adeno-Associated Virus Vector Production Using Frozen High-Density HEK-293 C
Scalable Lentiviral Vector Production Using Stable HEK293SF Producer Cell Lines
Development of Versatile and Flexible Sf9 Packaging Cell Line-Dependent OneBac System for Large-Scale Recombinant Adeno-
rAAV Production and Titration at the Microscale for High-Throughput Screening.
Detailed Protocol for the Novel and Scalable Viral Vector Upstream Process for AAV Gene Therapy Manufacturing.
Protocol for Efficient Generation and Characterization of Adeno-Associated Viral Vectors.
Benchmarking of Scale-X Bioreactor System in Lentiviral and Adenoviral Vector Production
Modifiers of Adeno-Associated Virus-Mediated Gene Expression in Implication for Serotype-Universal Neutralizing Antibody
Adeno-Associated Virus Vector Mobilization, Risk Versus Reality
Deciphering Key Adenoviral Elements in the Production of Recombinant Adeno-Associated Virus Vectors.
Gene Transfer of ZMapp Antibodies Mediated by Recombinant Adeno-Associated Virus Protects Against Ebola Infections.
Production of Recombinant Adeno-Associated Virus Through High-Cell-Density Transfection of HEK293 Cells Based on Fed-Per
British Society for Gene and Cell Therapy Annual Conference Wednesday 19th June – Friday 21st June 2019 University of Sh
Suppression of CNS APOE4 Expression by miRNAs Delivered by the S2 AAVrh.10 Capsid-Modified AAV Vector.
Neuro293: A REST-knockout HEK-293 cell line enables the expression of neuron-restricted genes for the high-throughput te
Significant Differences in Capsid Properties and Potency Between Adeno-Associated Virus Vectors Produced in Sf9 and HEK2
Human and Insect Cell-Produced Recombinant Adeno-Associated Viruses Show Differences in Genome Heterogeneity.
Magnetic resonance imaging of infarct-induced canonical wingless/integrated (Wnt)/β-catenin/T-cell factor pathway activa
Adeno-Associated Virus Monoinfection Induces a DNA Damage Response and DNA Repair That Contributes to Viral DNA Replicat
Production of Recombinant Adeno-Associated Viruses (rAAVs) by Transient Transfection.
Human Bocavirus 1 Is a Novel Helper for Adeno-associated Virus Replication.
Regulation of Blood Pressure by Targeting CaV1.2-Galectin-1 Protein Interaction.
The HPV16 E1 Carboxyl Domain Provides a Helper Function for Adeno-Associated Virus Replication.
Improved Genome Packaging Efficiency of Adeno-associated Virus Vectors Using Rep Hybrids.
[A recombinant adeno-associated virus expressing secretory TGF-β type Ⅱ receptor inhibits triple-negative murine breast
Can novel bioreactors improve the cost of goods of viral vectors?
Factors affecting recombinant Adeno-Associated Virus titers during triple-plasmid transient transfection in HEK-293 cell
Characterizing the Biopotency of Truncated Transgene Variants in rAAV8 viral vectors: Essential Considerations for Gene
Delivery of dCas9 CRISPR System Into the Hard Transfection Cells by Magnetofection Approach
ONLY A SMALL FRACTION OF CELLS PRODUCE ASSEMBLED CAPSIDS DURING TRANSFECTION-BASED MANUFACTURING OF ADENO-ASSOCIATED VIR
Advancements in Insect-Cell Baculovirus Expression Vector Platform for Production of Recombinant Adeno-Associated Virus-
Increased recombinant AAV production by HEK293 cells using small molecule chemical additives
Engineering pseudovirions for large-scale targeted gene transfer and recombinant adeno-associated virus production
High-cell-density production of adeno-associated viral vector serotype 6 by triple transfection in suspension HEK293 cel
Downregulation of α-Synuclein Protein Levels by an Intracellular Single-Chain Antibody.
Process Improvement of Adeno-Associated Virus Production
Establishment of a Recombinant AAV2/HBoV1 Vector Production System in Insect Cells.
Affecting HEK293 Cell Growth and Production Performance by Modifying the Expression of Specific Genes
A Direct Comparison of rAAV5 Variants Derived from the Baculovirus Expression System Using LC-MS Workflows Demonstrates
Synthetic Approaches for Nucleic Acid Delivery: Choosing the Right Carriers
Purification of Adeno-Associated Virus (AAV) Serotype 2 from Spodoptera frugiperda (Sf9) Lysate by Chromatographic Nonwo
Proteomic Landscape of Adeno-Associated Virus (AAV)-Producing HEK293 Cells.
Development of Stable Packaging and Producer Cell Lines for the Production of AAV Vectors.
Nuclear Fraction Proteome Analyses During rAAV Production of AAV2-Plasmid-Transfected HEK-293 Cells.
Biodegradable Polymers for Gene Delivery
Bioengineered Hybrid Rep 2/6 Gene Improves Encapsulation of a Single-Stranded Expression Cassette into AAV6 Vectors
Dielectric Spectroscopy to Improve the Production of rAAV Used in Gene Therapy
Production of an Oncolytic Adeno-Associated Virus Containing the Pro-Apoptotic TRAIL Gene Can Be Improved by shRNA Inter
Engineered AAV2.7m8 Serotype Shows Significantly Higher Transduction Efficiency of ARPE-19 and HEK293 Cell Lines Compare
Large-Scale Production of Lentiviral Vectors: Current Perspectives and Challenges
[Optimized AAV package and experimental application of recombinant AAV8/hFⅧ for gene therapy on hemophilia A mice].
In-Depth Comparison of Adeno-Associated Virus Containing Fractions after CsCl Ultracentrifugation Gradient Separation
AAV Vectors Pseudotyped with Capsids from Porcine and Bovine Species Mediate In Vitro and In Vivo Gene Delivery
Faculty Opinions recommendation of Adeno-associated virus vector as a platform for gene therapy delivery.
Comprehensive Comparison of Baculoviral and Plasmid Gene Delivery in Mammalian Cells
High-Level rAAV Vector Production by rAdV-Mediated Amplification of Small Amounts of Input Vector
Production of High-Yield Adeno Associated Vector Batches Using HEK293 Suspension Cells.
Characterization of Extracellular Vesicles Secreted in Lentiviral Producing HEK293SF Cell Cultures
Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors
JoVE Video Dataset
Engineered genetic tools for directed gene regulation
Adeno-Associated Virus Production in Serum-Free Suspension Adapted HEK293 Cells for Gene Therapy.
Development of a multiplexed RNAi-coupled sensor assay to study neuronal function on the large-scale
Development of a Therapeutic CRISPR/Cas9 Platform for Duchenne Muscular Dystrophy
Engineering of the CMV promoter for controlled expression of recombinant genes in HEK293 cells
[Expression and Regulatory Effect of miR-30b on Dynamin in Cochlear Hair Cells].
[Resistance of rAAV2-MfE77.43-Transferred Mice to Schistosoma japonicum Infection].
Tuning capsid formation dynamics in recombinant adeno‐associated virus producing synthetic cell lines to enhance full pa
Unraveling Cytotoxicity in HEK293 Cells During Recombinant AAV Production for Gene Therapy Applications.
Towards a scalable bioprocess for rAAV production using a HeLa stable cell line.
Manufacturing of recombinant adeno-associated viruses using mammalian expression platforms.
Rational plasmid design and bioprocess optimization to enhance recombinant adeno-associated virus (AAV) productivity in
Engineering of High-Yield Recombinant Adeno-Associated Virus Producer Plasmids.
Production of adeno-associated viral vector serotype 6 by triple transfection of suspension HEK293 cells at higher cell
Enhancing the production of adeno-associated virus (AAV)2 and AAV9 with high full capsid ratio in HEK293 cells through d
Advancements in molecular design and bioprocessing of recombinant adeno‐associated virus gene delivery vectors using the
Comprehensive mRNA-sequencing-based characterization of three HEK-293 cell lines during an rAAV production process for g
Cas-CLOVER-mediated knockout of STAT1: A novel approach to engineer packaging HEK-293 cell lines used for rAAV productio
Knockout of Pro-Apoptotic BAX and BAK1 Genes in HEK293T Cells Enhances Adeno-Associated Virus (AAV) Production: AAV2 and
Intensification of rAAV Production Based on HEK293 Cell Transient Transfection.
Critical challenges and advances in recombinant adeno‐associated virus (rAAV) biomanufacturing
Enhanced Adeno‐Associated Virus Production in HEK293 Cells via Dual Inducible Control of Cap and Rep Proteins
Molecular design of controllable recombinant adeno-associated virus (AAV) expression systems for enhanced vector product
Development of an HEK293 Suspension Cell Culture Medium, Transient Transfection Optimization Workflow, and Analytics for
Expression of Viral DNA Polymerase in Synthetic Recombinant Adeno-Associated Virus Producer Cell Line Enhances Full Part
Comparative Analysis of HEK293 Genomic Variability.
Multidose transient transfection of human embryonic kidney 293 cells modulates recombinant adeno-associated virus2/5 Rep
Accelerated Adeno Associated Virus Upstream Process Development From High‐Throughput Systems to Clinical Scale
Design space determination to optimize DNA complexation and full capsid formation in transient rAAV manufacturing
Iterative hybrid model based optimization of rAAV production.
High-yield recombinant adeno-associated viral vector production by multivariate optimization of bioprocess and transfect
Harnessing cell aggregates for enhanced adeno-associated virus manufacturing: Cultivation strategies and scale-up consid
Transcriptomic functional characterization of recombinant adeno-associated virus producing cell line adapted to suspensi
Improving the expression yield of recombinant adeno-associated virus serotype 2 using dimethyl sulfoxide DMSO as an addi
A synthetic platform for developing recombinant adeno-associated virus type 8 producer cell lines.
AAV Gene Transfer to the Heart.
Perfusion-Based Production of rAAV via an Intensified Transient Transfection Process.
Extracellular vesicles enhance pulmonary transduction of stably associated adeno‐associated virus following intratrachea
Production and Purification of Adeno-Associated Viral Vectors (AAVs) Using Orbitally Shaken HEK293 Cells.
Adeno-Associated Virus Production, Purification, and Titering.
Scalable Production and Purification of Adeno-Associated Viral Vectors (AAV).
A Rapid, Cost-Effective Method to Prepare Recombinant Adeno-Associated Virus for Efficient Gene Transfer to the Developi
Real-Time VCC Monitoring and Forecasting in HEK-Cell-Based rAAV Vector Production Using Capacitance Spectroscopy.
AAV Production Everywhere: A Simple, Fast, and Reliable Protocol for In-house AAV Vector Production Based on Chloroform
AAV Vectors for Efficient Gene Delivery to Rodent Hearts.
In Vivo Delivery of Cassettes Encoding Anti-HBV Primary MicroRNAs Using an Ancestral Adeno-Associated Viral Vector.
Silencing Genes in the Heart.
Production and Characterization of Vectors Based on the Cardiotropic AAV Serotype 9.
A feasibility study of different commercially available serum-free mediums to enhance lentivirus and adeno-associated vi
Exploring the design space of AAV transient-transfection in suspension cell lines.
Improved productivity of recombinant adeno-associated virus (rAAV) via triple transfection of HEK293 cells using perfusi
The use of melittin to enhance transgene expression mediated by recombinant adeno-associated virus serotype 2 vectors bo
The Unveiled Novel regulator of Adeno-associated virus production in HEK293 cells.
Immunogenicity of an adeno-vector vaccine expressing the F protein of a respiratory syncytial virus manufactured from se
Orthogonal characterization of rAAV9 reveals unexpected transgene heterogeneity.
Potency by design: Novel insights in transfection and purification for manufacturing of rAAV gene therapy vectors.
Therapeutic potential of AAV8-mediated IL-17RA extracellular domain gene delivery in an imiquimod-induced mouse model of
Efficient production of an avian adeno-associated virus vector using insect cell/baculovirus expression system.
Noninvasive focused ultrasound-mediated delivery of rAAV9-EGFP vectors for neuronal targeting in rats.
DNAJB6 suppresses alpha-synuclein induced pathology in an animal model of Parkinson's disease.
Mechanistic model for production of recombinant adeno-associated virus via triple transfection of HEK293 cells.
AAV2-mediated ABD-FGF21 gene delivery produces a sustained anti-hyperglycemic effect in type 2 diabetic mouse.
Preclinical Development of an AAV8-hUGT1A1 Vector for the Treatment of Crigler-Najjar Syndrome.
Scalable Production of AAV Vectors in Orbitally Shaken HEK293 Cells.
Recombinant AAV genome size effect on viral vector production, purification, and thermostability.
Production of rAAV by plasmid transfection induces antiviral and inflammatory responses in suspension HEK293 cells.
Methods Matter: Standard Production Platforms for Recombinant AAV Produce Chemically and Functionally Distinct Vectors.
Optimization of lentiviral vector production for scale-up in fixed-bed bioreactor
Analytical characterization of full, intermediate, and empty AAV capsids.
Gene therapy conversion of striatal astrocytes into GABAergic neurons in mouse models of Huntington's disease.
AAVvector-mediated in vivo reprogramming into pluripotency.
Self-attenuating adenovirus enables production of recombinant adeno-associated virus for high manufacturing yield withou
Adeno-associated virus capsid assembly is divergent and stochastic
The membrane associated accessory protein is an adeno-associated viral egress factor
Engineering a synthetic gene circuit for high-performance inducible expression in mammalian systems
Heterogeneity in an adeno-associated virus transfection-based production process limits the production efficiency.
LentiPro26: novel stable cell lines for constitutive lentiviral vector production
Polyethylenimine based magnetic nanoparticles mediated non-viral CRISPR/Cas9 system for genome editing
CRISPR/Cas9 as an antiviral against Orthopoxviruses using an AAV vector.
Engineered CHO cells as a novel AAV production platform for gene therapy delivery
Production of adeno-associated virus vectors for in vitro and in vivo applications
Receptor-targeted liposome-peptide-siRNA nanoparticles represent an efficient delivery system for MRTF silencing in conj
Efficacy and Safety of rAAV2-ND4 Treatment for Leber's Hereditary Optic Neuropathy.
AAV Gene Therapy for MPS1-associated Corneal Blindness.
High-purity AAV vector production utilizing recombination-dependent minicircle formation and genetic coupling.
Intranasal gene therapy to prevent infection by SARS-CoV-2 variants
Adeno-associated virus capsid assembly is divergent and stochastic
Fast, efficient and virus-free generation of TRAC -replaced CAR T cells
The membrane associated accessory protein is an adeno-associated viral egress factor
Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors
Evolving Membrane-Associated Accessory Protein Variants for Improved Adeno-associated Virus Production
The CASwitch: a synthetic biology solution for high-performance inducible gene expression systems in biotechnology
Engineered retrovirus-like nanocarriers for messenger RNA delivery into neurons
Rational design of a compact CRISPR-Cas9 activator for AAV-mediated delivery
Two-Step Small Scale Purification of Recombinant Adeno-Associated Viruses
Profiling the impact of the promoters on CRISPR-Cas12a system in human cells
Analysis of the impact of pluronic acid on the thermal stability and infectivity of AAV6.2FF.
Clusterin secreted from astrocyte promotes excitatory synaptic transmission and ameliorates Alzheimer's disease neu
Advanced biomanufacturing and evaluation of adeno-associated virus
Development of a perfusion process for serum-free adenovirus vector herpes zoster vaccine production
Utilizing minimally purified secreted rAAV for rapid and cost-effective manipulation of gene expression in the CNS.
Molecular barcoding of viral vectors enables mapping and optimization of mRNA trans -splicing
Potent immunogenicity and neutralization of recombinant adeno-associated virus expressing the glycoprotein of severe fev
Production of Adeno-Associated Virus Vector Serotype rh.10 and Optimization of Its Purification via Chloroform Extractio
Eukaryotic-driven directed evolution of Cas9 nucleases
Recombinant AAV Vectors for Enhanced Expression of Authentic IgG.
A single dose recombinant AAV based CHIKV vaccine elicits robust and durable protective antibody responses in mice.
Enhanced Transgene Expression from Recombinant Single-Stranded D-Sequence-Substituted Adeno-Associated Virus Vectors in
Characterization of Extracellular Vesicles Secreted in Lentiviral Producing HEK293SF Cell Cultures
AAV process intensification by perfusion bioreaction and integrated clarification
Targeted UDP-glucose ceramide glucosyltransferase stable overexpression induces a metabolic switch improving cell perfor
Quantitative analysis of preferential utilization of AAV ITR as the packaging terminal signal.
AAV8 vector induced gliosis following neuronal transgene expression.
AAV9-mediated gene delivery to liver grafts during static cold storage in a rat liver transplant model.
Directed evolution of AAV capsids for improved efficacy and specificity of delivery to preclinical models of human liver
Bioprocess Development and Bioreactor Scale-Up for the Production of Recombinant Lentiviral Viral Vectors in HEK293 Susp
Production of recombinant vesicular stomatitis virus-based vectors by tangential flow depth filtration
Proton-transfer-reaction mass spectrometry (PTR-MS) for online monitoring of glucose depletion and cell concentrations i
Enhanced ER protein processing gene expression increases rAAV yield and full capsid ratio in HEK293 cells.
Factors affecting rAAV titers during triple-plasmid transient transfection in HEK-293 cells.
Targeted knockdown of ATM, ATR, and PDEδ increases Gag HIV-1 VLP production in HEK293 cells
Production of retroviral vectors in continuous high cell density culture
Pharmaceutical Development of AAV-Based Gene Therapy Products for the Eye
Comparative transcriptomic and proteomic kinetic analysis of adeno-associated virus production systems.
Adenoviral vectors for cardiovascular gene therapy applications: a clinical and industry perspective
Enhancer viruses and a transgenic platform for combinatorial cell subclass-specific labeling
Targeted overexpression of relaxin receptor 1 (RXFP1) with chronic administration of relaxin as a novel inotropic approa
Abstracts from the 25th European Society for Animal Cell Technology Meeting: Cell Technologies for Innovative Therapies
With small viruses come giant responsibilities - Next-generation parvoviral vectors for human gene therapy with extended
Development of AAV-mediated gene therapy for autosomal recessive bestrophinopathy
Culture media selection and feeding strategy for high titer production of a lentiviral vector by stable producer clones
Improving HEK293-based AAV-production using GSMMs, and a multi-omics approach